Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar)

• Autorzy: Agrawal A. , Sanayaima R. , Tandon R. , Tyagi R. K.
• Języki publikacji: EN

Abstrakty

EN

To decrease the cost of in vitro conservation of banana cv. Karpura Chakkarakeli (AAB; Mysore sub-group) without any adverse effects on cultures, expensive components of medium such as sucrose and gelling agents, i.e. phytagel or agar (90% of the total cost of the medium), were replaced with inexpensive alternates such as market sugar and isabgol, respectively (Experiment 1). In general, no significant effects of isabgol and market sugar were observed on shoot (1.0–1.3 shoots/shoot explant) and root (1.5–2.0 roots/shoot explant) regeneration. Up to 12 months, 100% of cultures survived on isabgol-media, which was significantly higher than that on agar-media (79–83%) and on phytagel-media (51–57%). Isabgol-media with or without other constituents of medium were also tested for survival of banana cultures (Experiment 2); significant differences were observed for survival of cultures (20–100%). Slow growth of the cultures on isabgol-media was attributed to low availability of free water and consequently slower rate of transport of nutrients from isabgol matrix to the plantlets than that of other media tested, as evidenced by significantly lower relative matric potentials (0.801 and 0.804) of isabgol-media. In vitro conservation-derived plants grown in the field exhibited no significant morphological variations. The total cost of medium used for in vitro conservation of banana was decreased by 59% by using isabgol as an alternate gelling agent to agar and phytagel.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 4
• Opis fizyczny: p.703-711,fig.,ref.

Twórcy

autor

Agrawal A.

• Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India

autor

Sanayaima R.

• Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
• Department of Botany, University of Delhi, Delhi 110007, India

autor

Tandon R.

• Department of Botany, University of Delhi, Delhi 110007, India

autor

Tyagi R. K.

• Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India

Bibliografia

• Agrawal A, Swennen R, Panis B (2004) A comparison of four methods for cryopreservation of meristems in banana (Musa spp.). CryoLetters 25:101–110
• Agrawal A, Tyagi RK, Goswami R (2008) Cryopreservation of subgroup Monthan (ABB) of Indian cooking bananas (Musa spp.). Curr Sci 94:1125–1128
• Ashmore SE (1997) Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. International Plant Genetic Resources Institute, Rome, 67 pp
• Babbar SB, Jain R (1998) ‘Isubgol’ as an alternative gelling agent in plant tissue culture media. Plant Cell Rep 17:318–322
• Banerjee N, de Langhe EA (1985) Tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (banana and plantain). Plant Cell Rep 4:351–354
• Beruto D, Beruto M, Ciccarelli C, Debergh P (1995) Matric potential evaluations and measurements of gelled substrates. Physiol Plant 94:151–157
• Bhat SR, Chandel KPS (1993) In vitro conservation of Musa germplasm: effects of mannitol and temperature on growth and storage. J Hort Sci 68:841–846
• Bhattacharya P, Dey S, Bhattacharyya BC (1994) Use of low-cost gelling agents and support matrices for industrial scale plant tissue culture. Plant Cell Tissue Org Cult 37:15–23
• Cameron SI (2006) Tissue culture gel firmness: measurement and effects on growth. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, The Netherlands, pp 329–337
• Côte FX, Folliot M, Domergue R, Dubois C (2000) Field performance of embryogenic cell suspension-derived banana plants (Musa AAA, cv. Grande Naine). Euphytica 112:245–251
• Craeyveld VV, Delcour JA, Coutrin CM (2009) Extractability and chemical and enzymatic degradation of psyllium (Plantago ovata Forsk) seed husk arabinoxylans. Food Chem 112:812–819
• Debergh PC (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol Plant 9:270–276
• Eberhard S, Doubrava N, Marfa V, Mohnen D, Southwick A, Darvill A, Albersheim P (1989) Pectic cell wall fragments regulate tobacco thin-cell-layer explant morphogenesis. Plant Cell 1:747–755
• FAO (2005) http://faostat.fao.org/site/336/DesktopDefault.aspx?PageID=336
• Fischer MH, Yu N, Gary GR, Ralph J, Anderson L, Marlett JA (2004) The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk). Carbohydr Res 339:2009–2017
• Ganapathi TR, Mohan ISS, Suprasanna P, Bapat VA, Rao PS (1995) A low-cost strategy for in vitro propagation of banana. Curr Sci 68:646–650
• Goel MK, Kukreja AK, Khanuja SPS (2007) Cost-effective approaches for in vitro mass propagation of Rauwolfia serpentina Benth. Ex Kurz. Asian J Plant Sci 6:957–961
• INIBAP (2006) Global conservation strategy for Musa (Banana and Plantain). International Network for Improvement of Banana and Plantain, Montpellier, 27 pp
• IPGRI-INIBAP/CIRAD (1996) Descriptors for banana (Musa spp.). International Plant Genetic Resources Institute, Rome, Italy/International Network for the Improvement of Banana and Plantain, Montpellier, France/Centre de coopération international en recherche agronomique pour le développment, Montpellier, 55 pp
• Israeli Y, Reuveni O, Lahav E (1991) Qualitative aspects of somaclonal variations in banana propagated by in vitro techniques. Sci Hortic 48:71–88
• Klimaszewska K, Smith DR (1997) Maturation of somatic embryos of Pinus strobus is promoted by a high concentration of gellan gum. Physiol Plant 100:949–957
• Kodym A, Zapata-Arias FJ (2001) Low-cost alternatives for the micropropagation of banana. Plant Cell Tissue Org Cult 66:67–71
• Letarte J, Simion E, Miner M, Kasha KJ (2006) Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24:691–698
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
• Ortiz R (1997) Morphological variation in Musa germplasm. Genet Resour Crop Evol 44:393–404
• Owens LD, Wozniak CA (1991) Measurement and effects of gel matric potential and expressibility on production of morphogenic callus by cultured sugarbeet leaf discs. Plant Cell Tissue Org Cult 26:127–133
• Panis B (2009) Cryopreservation of Musa germplasm, 2nd edn. In: Engelmann F, Benson E (eds) Technical guidelines No. 9. Biodiversity International, Montpellier, 48 pp
• Panis B, Thinh NT (2001) Cryopreservation of Musa germplasm. In: Escalant JV, Sharrock S (eds) INIBAP technical guidelines. International Network for the Improvement of Banana and Plantain, Montpellier, 44 pp
• Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristem: a cryopreservation protocol applicable to all Musaceae. Plant Sci 168:45–55
• Rizvi MZ, Kukreja AK, Khanuja SPS (2007) In vitro culture of Chlorophytum borivilianum Sant. Et Fernand in liquid culture medium as a cost-effective measure. Curr Sci 92:87–90
• Tyagi RK, Agarwal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma Longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell Dev Biol - Plant 43:51–58
• Uma S, Siva SA, Saraswathi MS, Manickavasagam M, Durai P, Selvarajan R, Sathiamoorthy S (2006) Variation and interspecific relationships in Indian wild Musa balbisiana (BB) population as evidenced by random amplified polymorphic DNA. Genet Resour Crop Evol 53:349–355
• Van den Houwe I, De Smet K, Tenezas du Montcel H, Swennen R (1995) Variability in storage potential of banana shoot cultures under medium term storage conditions. Plant Cell Tissue Org Cult 42:269–274
• Withers LA (1991) In vitro conservation. Biol J Linnean Soc 43:31–42