Enzymatic activity of Prototheca zopfii strains isolated from cows with mastitis

• Autorzy: Krukowski H. , Lisowski A. , Nowakowicz-Debek B. , Wlazlo L.
• Języki publikacji: EN

Abstrakty

EN

Bovine mastitis caused by Prototheca spp. can be a disease of high significance because of economic losses and the potential risk to public health. e aim of our study was to evaluate enzymatic activity of Prototheca zopfii. For this study, we used 15 P. zopfii strains previously isolated from cows with clinical and subclinical mastitis in Poland. We determined enzymatic profile of Prototheca species using the API ZYM system. Of the enzymatic activities detected during the study, acid phosphatase, leucine arylamidase, naphthol-as-bi-phosphohydrolase, esterase, lipase esterase, valine arylamidase, alkaline phosphatase, and lipase C14 were found in high percentage of strains.

Słowa kluczowe

EN

enzyme activity; Prototheca zopfii; cow; mastitis; economic loss; health risk; public health; acid phosphatase; leucine arylamidase; esterase; lipase esterase; alkaline phosphatase

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 3
• Opis fizyczny: p.217-218,ref.

Twórcy

autor

Krukowski H.

• Department of Animal and Environmental Hygiene, University of Life Sciences, Lublin, Poland

autor

Lisowski A.

autor

Nowakowicz-Debek B.

autor

Wlazlo L.

Bibliografia

• Brasch J. and M. Zaldua. 1994: Enzyme patterns of dermatophytes. Mycoses. 37: 11–16.
• Bueno V.F.F., A.J. de Mesquita, R.B.S. Neves, M.A. de Souza, A.R. Ribeiro, E.S. Nicolau and A.N. de Oliveira. 2006. Epidemiological and clinical aspects of the first outbreak of bovine mastitis caused by Prototheca zopfii in Goia’s State, Brazil. Mycopathologia, 161: 141–145.
• Casal M.J. and J. Gutierrez. 1983. Simple new test for rapid differentiation of Prototheca wickerhamii from Prototheca zopfi. J. Clin. Microbiol. 18: 992–993.
• Casal M., M.J. Linares and M.M. Morales. 1985. Enzymatic profile of Prototheca species. Mycopathologia, 92: 81–82.
• Costa E.O., A.R. Ribeiro, P.A. Melville, M.S. Prada, A.C. Carciofi, and E.T. Watanabe. 1996. Bovine mastitis due to algae of the genus Prototheca. Mycopathologia, 137: 85–88.
• Costa E.O., P.A. Melville, A.R. Ribeiro, E.T. Watanabe and M.C.F.F. Parolari. 1997. Epidemiologic study of environmental sources in a Prototheca zopfii outbreak of bovine mastitis. Mycopathologia, 137: 33–36.
• Cunha L.T., S.M.P. Pugine, M.R.M. Silva, E.J.X. Costa and M.P. Melo. 2010. Microbicidal action of indole-3-acetic acid combined with horseradish peroxidase on Prototheca zopfii from bovine mastitis. Mycopathologia, 169: 99–105.
• Cunha L.T., S.P. Pugine, C.R. Valle, A.R. Ribeiro, E.J.X. Costa and M.P. De Melo. 2006. Effect of Prototheca zopfii on neutrophil function from bovine milk. Mycopathologia, 162: 421–426.
• Galan F., P.G. Martos, M.J. Palomo, M.Beltran, J.L. Gil and J. Mira. 1997. Onychoprotothecosis due to Prototheca wickerhami. Mycopathologia, 137: 75–77.
• Jagielski T., H. Lassa, J. Ahrholdt, E. Malinowski E. and U. Rosler. 2011. Genotyping of bovine Prototheca mastitis isolates from Poland. Vet. Microbiol. 149: 283–287.
• Jagielski T., H. Lassa, J. Ahrholdt, U. Rosler and E. Malinowski. 2010. Molecular characterization of Polish Prototheca zopfii mastitis isolates and first isolation of Prototheca blaschkeae in Poland. Pol. J. Vet. Sci. 13: 725–729.
• Kirk J. and R. Mellenberger. Mastitis control program for Prototheca mastitis in dairy cows” http://www.uwex.edu/milkquality/PDF/prototheca.pdf
• Krajewska-Kułak E., C. Łukaszuk, W. Niczyporuk and J. Trybuła. 1998. Evaluation of activity of selected hydrolytic enzymes of the yeast-like fungi of Candida isolated from urether. Mikol. Lek. 5: 165–170 (in Polish)
• Lass-Flörl C. and A. Mayr. 2007. Human protothecosis. Clin. Microbiol. Rev. 20: 230–242,.
• Lassa H., T. Jagielski and E. Malinowski. 2011. Effect of different heat treatments and disinfectants on the survival of Prototheca zopfii. Mycopathologia, 171: 177–182.
• Malinowski E., H. Lassa and A. Kłossowska. 2002. Isolation of Prototheca zopfii from inflamed secretion of udders. Bull. Vet. Inst. Pulawy, 46: 295–299.
• Marques S., E. Silva, J. Carvalheira and G. Thompson. 2010. In vitro susceptibility of Prototheca to pH and salt concentration. Mycopathologia, 169: 297–302,
• Marques S., E. Silva, J. Carvalheira and G. Thompson. 2006. In vitro antimicrobial susceptibility of Prototheca wickerhamii and Prototheca zopfii isolated from bovine mastitis. J. Dairy Sci. 89: 4202–4204.
• Padhye A.A., J.G. Baker and D.F. D’amato. 1979. Rapid identification of Prototheca species by the API 20C System. J. Clin. Microbiol. 10: 579–582.
• Roesler U., A. Moller, A. Hensel, D. Baumann and U. Truyen. 2006. Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov. Int. J. Syst. Evol. Microbiol. 56: 419–1425.