The expression profile of LmTRYP, LmTRYR, and LmHSP 83 genes in treatment failure clinical isolates of Leishmania major

• Autorzy: Nourbakhsh A. , Eslami G. , Sohrevardi S.M. , Vakili M.
• Języki publikacji: EN

Abstrakty

EN

Cutaneous leishmaniosis (CL) is one of the important neglected tropical diseases caused by Leishmania spp. such as L. major, L. tropica in the Old World. In recent years, some reports of treatment failure in patients with CL have been reported worldwide. Therefore, in this study, we assessed LmHSP 83, LmTRYR, and LmTRYP gene expressions in treatment failure clinical isolates of L. major. After sampling from the cutaneous lesions, DNA was extracted and then genera verification and species identification was done using ITS1-PCR-RFLP method. A part of each sample was used in order to RNA extraction and cDNA synthesized. LmHSP 83, LmTRYR, and LmTRYP gene expressions were assessed using SYBR Green real-time PCR. The treatment failure clinical isolates had the mean expression of 5.55±1.67, 247.024±23.54, and 1.204±2.14 for LmHSP 83, LmTRYR, and LmTRYP , respectively less than the same genes in treatment response isolates (P=0.001). This study recommended the other mechanisms may involve in response to treatment in treatment failure clinical isolates of L. major.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2021
• Tom: 67
• Numer: 4
• Opis fizyczny: p.749-755,fig.,ref.

Twórcy

autor

Nourbakhsh A.

• Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
• Department of Clinical Pharmacy, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

autor

Eslami G.

• Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

autor

Sohrevardi S.M.

• Department of Clinical Pharmacy, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

autor

Vakili M.

• Department of Community and Preventive Medicine, Health Monitoring Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Identyfikatory

DOI

10.17420/ap6704.392