UVA radiation enhancement of beta-carotene yields in Rhodotorula mucilaginosa

• Autorzy: Bogacz-Radomska L. , Harasym J.

Warianty tytułu

PL

Zwiększenie produkcji beta-karotenu w drożdżach Rhodotorula mucilaginosa pod wpływem promieniowania UVA

• Języki publikacji: EN

Abstrakty

EN

The influence of UVA and VIS radiation on the β-carotene biosynthesis by yeast Rhodotorula mucilaginosa was studied. The impact of radiation characteristic on biomass growth, substrate consumption and β-carotene production in submerged batch culture in bioreactor was studied. Three different types of light sources were used in the experiment: VIS – 15 W, wavelength range from 400 to 800 nm, the largest emission at 420 nm; UVA – 18 W, blue light, wavelength range of 350 to 430 nm, the largest emission at 370 nm; UVA – 20 W, wavelength range from 350 to 575 nm, the largest emission at 350 nm. In cultures conducted with UVA – 18 W radiation the highest β-carotene and carotenoids cellular concentration were achieved, respectively 63.20 μg/gd.w. and 0.97 mg/gd.w.. In cultures subject to VIS radiation a lower β-carotene concentration at 43.60 μg/gd.w. and the total carotenoids concentration at 0.83 mg/gd.w. was obtained. The ultraviolet radiation with the emission peak of radiation at 370 nm promoted the β-carotene and carotenoids production in yeast Rhodotorula mucilaginosa.

PL

Badano wpływ promieniowania UVA i VIS na biosyntezę β-karotenu przez drożdże Rhodotorula mucilaginosa. Określano wpływ promieniowania charakterystycznego na wzrost biomasy, zużycie substratu i produkcję β-karotenu we wgłębnej hodowli okresowej prowadzonej w bioreaktorze. Zastosowano trzy różne rodzaje źródeł światła: VIS – 15 W, zakres długości fali od 400 do 800 nm, największa emisja przy 420 nm; UVA1 – 18 W, zakres długości fali od 350 do 430 nm, największa emisja przy 370 nm; UVA2 – 20 W, zakres długości fali od 350 do 575 nm, największa emisja przy 350 nm. W kulturach prowadzonych za pomocą promieniowania UVA otrzymano najwyższe stężenie β-karotenu i karotenoidów, odpowiednio 63,20 μg/gs.m. i 0,97 mg/gs.m. W hodowlach napromieniowanych lampą VIS uzyskano niższe stężenie β-karotenu (43,60 μg/gs.m) i karotenoidów (0,83 mg/gs.m). Promieniowanie ultrafioletowe ze szczytem emisji promieniowania przy 370 nm promowało produkcję β-karotenu i karotenoidów w komórkach drożdży Rhodotorula mucilaginosa.

Słowa kluczowe

EN

UV radiation zob.ultraviolet radiation; ultraviolet radiation; beta-carotene; carotenoid; yield; Rhodotorula mucilaginosa; yeast; visible spectrum; VIS radiation zob.visible spectrum

• Wydawca: -
• Czasopismo: Nauki Inżynierskie i Technologie
• Rocznik: 2016
• Numer: 3(22)
• Opis fizyczny: p.9-20,fig.,ref.

Twórcy

autor

Bogacz-Radomska L.

• Wroclaw University of Economics, Wroclaw, Poland

autor

Harasym J.

• Wroclaw University of Economics, Wroclaw, Poland
• University of Valladolid, C/Plaza de Santa Cruz, 8, 47002 Valladolid, Spain

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Identyfikatory

DOI

10.15611/nit.2016.3.01