Characterization of p-hydroxybenzaldehyde dehydrogenase, the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota

• Autorzy: Sircar D. , Mukherjee C. , Beuerle T. , Mitra A.
• Języki publikacji: EN

Abstrakty

EN

Hairy root cultures of Daucus carota respond to methyl-jasmonate treatment with enhanced accumulation of p-hydroxybenzoic acid. The final C₁-side chain of this compound is shaped by p-hydroxybenzaldehyde dehydrogenase (HBD) that catalyzes the formation of p-hydroxybenzoic acid from p-hydroxybenzaldehyde in the presence of NAD⁺. HBD was biochemically characterized from cell-free hairy root extracts of D. carota. The preferred substrate for HBD was p-hydroxybenzaldehyde. The apparent Km values were 54.8 and 74.4 µM for p-hydroxybenzaldehyde and NAD⁺, respectively. Divalent metal cations did not significantly affect enzyme activity.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.2019-2024,fig.,ref.

Twórcy

autor

Sircar D.

• Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

autor

Mukherjee C.

• Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

autor

Beuerle T.

• Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstrabe 1, 38106, Brunswick, Germany

autor

Mitra A.

• Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

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