Sucrose regulated enhanced induction of anthraquinone, phenolic, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.)

• Autorzy: Baque M.A. , Elgirban A. , Lee E.-J. , Paek K.-Y.
• Języki publikacji: EN

Abstrakty

EN

The effect of initial sucrose concentration was investigated in root suspension cultures of Morinda citrifolia to improve root growth and secondary metabolites production, i.e. Anthraquinone, phenolics and flavonoids. Besides, oxidative stress level, antioxidant enzymes activity and membranes damage under different sucrose concentration were estimated. A 5% sucrose supply was shown to be optimal for the production of root dry mass, but higher sucrose concentrations of 7–9% inhibited the accumulation of root dry weight (DW). However, the maximum production of anthraquinone (251.89 mg L⁻¹ DW), phenolics (165.14 mg L⁻¹ DW) and flavonoids (163.56 mg L⁻¹ DW) were achieved at 1% sucrose-treated culture, which may be a source carbon skeletons for secondary metabolism. At the same time was observed low oxidative damage, which could be associated with high levels of secondary metabolites and the increased activity of catalase. Although, catalase (CAT) activity were stimulated at 7–9% sucrose-treated cultures, high accumulation of hydrogen peroxide (H₂O₂) and peroxidation of lipid (MDA) was induced. The observed high activity of CAT and guaiacol peroxidase (G-POD) were not sufficient enough to mitigate the toxic effect of H₂O₂.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.405-415,fig.,ref.

Twórcy

autor

Baque M.A.

• Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheongju, Republic of Korea
• Department of Agronomy, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

autor

Elgirban A.

• Center of Excellence in Biotechnology Research, King Saud University, 11451 Riyadh, Kingdom of Saudi Arabia

autor

Lee E.-J.

• Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheongju, Republic of Korea
• CBN PLANTECH Co. Ltd., Industry Academic Cooperation Foudation Agribusiness Incubator Center 206, Chungbuk National University, 361-763 Cheongju, Republic of Korea

autor

Paek K.-Y.

• Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheongju, Republic of Korea

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