Quantitative changes in selected lymphocyte subpopulations after administration of a soluble parasitic antigen of Babesia Canis to dogs

• Autorzy: Adaszek L. , Lisiecka U. , Martinez A. C. , Skrzypczak M. , Winiarczyk S.
• Języki publikacji: EN

Abstrakty

EN

Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S RNA-B Babesia canis cell culture were investigated. The studies included 20 dogs divided into three groups: group I (n=8) - comprised of dogs receiving SPA twice, at 3 week intervals; group II (n=5) - nonvaccinated control dogs, and group III (n=7) - dogs vaccinated twice with a commercial B. canis vaccine. Cytometric analysis revealed that vaccination with SPA derived from B. canis culture had similar effects to the vaccination with a commercial vaccine. The vaccination lowered the percentage of T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic/supressor T cells (CD8+), B lymphocytes (CD21+), and MHC II lymphocytes in the blood in comparison to non-vaccinated dogs. Statistical analysis of the results demonstrated that mean values of the tested parameters at each stage of the study were similar in groups I and III and significantly higher in group II. The lowered level of the lymphocyte subpopulations in groups I and III persisted during the whole period of the study. The results presented that SPA has immunosuppressive effect in the first period after being administrated.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 1
• Opis fizyczny: p.35-39,fig.,ref.

Twórcy

autor

Adaszek L.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

autor

Lisiecka U.

• Animal Health Department, Edificio de Sanidad Animal Campus, Universitario de Rabanales, 14014 Cordoba, Spain

autor

Martinez A. C.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

autor

Skrzypczak M.

• Second Department of Gynecology, Prof. F. Skubiszewski University School of Medicine, 20-090 Lublin, Poland

autor

Winiarczyk S.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

Bibliografia

• 1. Adaszek Ł., Winiarczyk S.: Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet Parasitol 2008, 152, 235-241.
• 2. Adaszek Ł., Winiarczyk S., Skrzypczak M.: The clinical course of babesiosis in 76 dogs infected with protozoa parasites Babesia canis canis. Pol J Vet Sci 2009, 12, 81- 87.
• 3. Adaszek Ł., Winiarczyk S.: In vitro cultivation of Babesia canis canis parasites isolated from dogs in Poland. Parasitol Res 2011, 108, 1303-1307.
• 4. Adaszek Ł., Carbonero-Martinez A., Winiarczyk S.: The factors affecting the distribution of babesiosis in dogs in Poland. Vet Parasitol 2011, 81, 160-165.
• 5. Adaszek Ł., Wernicka-Furmaga R., Winiarczyk S.: Preliminary study on the safety of a new vaccine against canine babesiosis containing soluble parasitic antigen (SPA). Bull Vet Inst Pulawy 2012, 56, 145-148.
• 6. Ahmed J.S.: The role of cytokines in immunity and immunopathogenesis of piroplasmoses. Parasitol Res 2002, 88, 48-50.
• 7. Brown W.C., Logan K.S:. Babesia bovis: bovine helper T cell lines reactive with soluble and membrane antigens of merozoites. Exp Parasitol 1992, 74, 188-199.
• 8. Brown W.C., Ruef B.J., Norimine J., Kegerreis K.A., Suarez C.E., Conley P.G., Stich R.W., Carson K.H., Rice-Ficht A.C.: A novel 20-kilodalton protein conserved in Babesia bovis and B. bigemina stimulates memory CD4(+) T lymphocyte responses in B. bovis-immune cattle. Mol Biochem Parasitol 2001, 118, 97-109.
• 9. Carcy B., Précigout E., Schetters T., Gorenflot A.: Genetic basis for GPI-anchor merozoite surface antigen polymorphism of Babesia and resulting antigenic diversity. Vet Parasitol 2006, 138, 33-49.
• 10. De Lima V.M., Ikeda F.A., Rossi C.N., Feitosa M.M., Vasconcelos R.O., Nunes C.M., Goto H.:. Diminished CD4+/CD25+ T cell and increased IFN-y levels occur in dogs vaccinated with Leishmune in an endemic area for visceral leishmaniasis. Vet Immunol Immunopathol 2010, 135, 296-302.
• 11. Eckert J., Deplazes P.: Vaccines against parasitic diseases of domestic animals. Tierarztl Prax 1996, 24, 322-329.
• 12. García T.D., Figueroa M.J., Ramos A.J., Rojas M.C., Cantó A.G., Falcón N.A., Alvarez M.J.: Immune response to Babesia bigemina infection in pregnant cows. Ann N Y Acad Sci 2004, 1026, 298-301.
• 13. Laurent N., l'Etoile M.: Process for the culture of Babesia canis. Application to the preparation of antigens and vaccine against pyroplasmosis. Patent No. US 4,777,036. 1988.
• 14. Miyamoto T., Taura Y., Une S., Yoshitake M., Nakama S., Watanabe S.: Immunological responses after vaccination pre- and post-surgery in dogs. J Vet Med Sci 1995, 57, 29-32.
• 15. Schetters T.P., Kleuskens J., Scholtes N., Bos H.J.: Vaccination of dogs against Babesia canis infection using parasite antigens from in vitro culture. Parasite Immunol 1992, 14,295-305.
• 16. Schetters T.P., Montenegro-James S.: Vaccines against babesiosis using soluble parasite antigens. Parasitol Today 1995,11, 456-462.
• 17. Schetters T.P., Scholtes N.C., Kleuskens J.A., Bos H.J.: Not peripheral parasitaemia but the level of soluble parasite antigen in plasma correlates with vaccine efficacy against Babesia canis. Parasite Immunol 1996, 18, 1-6.
• 18. Schetters T.P., Kleuskens J.A., Scholtes N.C., Pasman J. W., Goovaerts D.: Vaccination of dogs against Babesia canis infection. Vet Parasitol 1997, 73, 35-41.
• 19. Schetters T.P., Kleuskens J.A., Scholtes N.C., Gorenflot A., Moubri K., Vermeulen A.N.: Vaccination of dogs against heterologous Babesia canis infection using antigens from culture supernatants. Vet Parasitol 2001, 100, 75-86.
• 20. Schetters T.P., Strydom T., Crafford D., Kleuskens J.A., van de Crommert J., Vermeulen A.N., Strasser A., May B., Teltscher A., Wistrela E., Niedermüller H.: Immune modulation following immunization with polyvalent vaccines in dogs. Vet Immunol Immunopathol 2003, 94, 113-121.
• 21. Schetters T.: Vaccination against canine babesiosis. Trends Parasitol 2005, 21, 179-184.
• 22. Schetters T.P., Kleuskens J., Carcy B., Gorenflot A., Vermeulen A.: Vaccination against large Babesia species from dogs. Parassitologia 2007, 49 Suppl 1, 13-17.
• 23. Schetters T.P., Strydom T., Crafford D., Kleuskens J.A., van de Crommert J., Vermeulen A.N.: Immunity against Babesia rossi infection in dogs vaccinated with antigens from culture supernatants. Vet Parasitol 2007, 144, 10- 19.
• 24. Schetters T.P., Moubri K., Cooke B.M.: Comparison of Babesia rossi and Babesia canis isolates with emphasis on effects of vaccination with soluble parasite antigens: a review. J S Afr Vet Assoc 2009, 80, 75-78.
• 25. Sibinovic K.H., Sibinovic S., Ristic M., Cox H.W.: Immunogenic properties of babesial serum antigens. J. Parasitai 1967, 53, 1121-1129.
• 26. Strasser A., May B., Teltscher A., Wistrela E., Niedermüller H.: Immune modulation following immunization with polyvalent vaccines in dogs. Vet Immunol Immunopathol 2003, 94, 113-121.
• 27. Zygner W., Wedrychowicz H.: Influence of anaemia on azotaemia in dogs infected with Babesia canis in Poland. Bull Vet Inst Pulawy 2009, 53, 663-668.
• 28. Zygner W., Gójska-Zygner O., Wedrychowicz H.: Abnormalities in serum proteins in the course of babesiosis in dogs. Bull Vet Inst Pulawy 2011, 55, 59-65.

Identyfikatory

DOI

10.2478/bvip-2013-0007