New data on the little-studied darkling beetle subfamily Phrenapatinae is given for the Afrotropical Region. The following new taxa are described and included into a key to the Phrenapatinae species of southern Africa: Archaeoglenes ruthmuelleraesp. nov. from the Republic of South Africa and the Republic of Mozambique, Endroeditagalusgen. nov.ongoyussp. nov. from the Republic of South Africa and Endroeditagalusgen. nov.ntsubanussp. nov. from the Republic of South Africa. New distributional data is incorporated into a checklist of the Afrotropical Phrenapatinae fauna.