PL
Dokonano selekcji 9 niskoproteazowych mutantów Trichoderma reesei M-7 do produkcji enzymów zewnątrzkomórkowych na podłożu z nierozcieńczoną serwatką wzbogaconą w sole mineralne. Hodowle fermentorowe prowadzono metodą ciągłą w bioreaktorze Bioflo III firmy New Brunswick Scientific Co. Inc. o pojemności roboczej 5 dm3. Stwierdzono odwrotnie proporcjonalne zależności pomiędzy produkcją celulaz i ksylanaz a proteaz w hodowlach niskoproteazowych mutantów Trichoderma reesei M-7 (M-7.6, M-7.8, M-7.10).
EN
An evaluation was made of the ability of 9 low-protease mutants of Trichoderma reesei M-7 to produce extracellular enzymes on a medium with whey enriched with mineral salts. Cultures were carried out using batch and continuous culture methods. Inversely proportional relationships between the activity of cellulases and xylanases as well as the activity of proteases in filtrates obtained from cultures of low-protease mutants of Trichoderma reesei M-7 were not found unambiguously in batch cultures. In continuous cultures, in turn, the highest activities were shown by filtrates of mutants of Trichoderma reesei M-7 which exhibited the lowest ability to produce extracellular proteases in batch cultures.