Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers

• Autorzy: Okon S. , Paczos-Grzeda E. , Loboda M. , Sugier D.

Warianty tytułu

PL

Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD

• Języki publikacji: EN

Abstrakty

EN

Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.

PL

Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.

Słowa kluczowe

EN

DNA polymorphism; identification; genetic diversity; Arnica montana; genotype; RAPD marker; medicinal plant; molecular analysis

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 4
• Opis fizyczny: p.63-71,fig.,ref.

Twórcy

autor

Okon S.

• Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Paczos-Grzeda E.

• Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Loboda M.

• Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Sugier D.

• Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science in Lublin, Akademicka 15, 20-950 Lublin, Poland

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