Role of IL-1beta in stimulation of peripheral mononuclear cells to secrete ET-1 and in development of vasospasm following subarachnoid hemorrhage in rats

• Autorzy: Jedrzejowska-Szypulka H. , Larysz-Borysz M. , Woszczycka-Korczynska I. , Lewin-Kowalik J.
• Języki publikacji: EN

Abstrakty

EN

Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. As a result, much pathology develops, including arterial vasospasm leading to neuronal ischemia resulting in neurological deficits. Cerebral arteries may be constricted both directly by cytokine influence on arterial smooth muscles and indirectly, through increase of endothelin-1 (ET-1) synthesis or adhesion molecules production. Interleukin-1β (IL-1β) is secreted following SAH and through broken bloodbrain barrier it may lead to the stimulation of peripheral leukocytes. These peripheral mononuclear cells (PMC) may be an additional source of ET-1 and migrating to the brain they can enhance the vasospasm. We examined effect of neutralization of IL-1β on secretion of ET-1 by PMCs as well as on basilar artery vasospasm in rats following SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies. Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, blood samples were collected from the extraorbital plexus and centrifuged to separate leukocyte subpopulations. Isolated PMCs (monocytes and lymphocytes) were cultured for 24 hrs and ET-1 concentration in the supernatants was assessed with ELISA. In brain stem specimens, vasospasm was determined. SAH led to the strong vasospasm and increase of production ET-1 by PMCs. Neutralization of IL-1β activity significantly reduced the ET-1 level in both time-points, but led to decrease of vasospasm only after 24 hrs. The results suggest that ET-1 does not influence the vasospasm in the acute phase but is involved in this process 24 hrs after subarachnoid hemorrhage.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.68

Twórcy

autor

Jedrzejowska-Szypulka H.

• Department Physiology, Medical University of Silesia, Katowice, Poland

autor

Larysz-Borysz M.

• Department Physiology, Medical University of Silesia, Katowice, Poland

autor

Woszczycka-Korczynska I.

• Department Physiology, Medical University of Silesia, Katowice, Poland

autor

Lewin-Kowalik J.

• Department Physiology, Medical University of Silesia, Katowice, Poland

Uwagi

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