Cryopreservation of shoot tips from the endangered endemic species Tuberaria major

• Autorzy: Coelho N. , Gonzalez-Benito M.E. , Romano A.
• Języki publikacji: EN

Abstrakty

EN

Tuberaria major is an endangered endemic species from the Algarve, in the south of Portugal. We investigated two techniques for the cryopreservation of T. major shoot tips, namely vitrification and encapsulation-dehydration. Before the cryopreservation trials, shoot tips were precultured for 1 day on liquid Murashige and Skoog (MS) medium containing 0.3 M sucrose. For the vitrification method, shoots tips were exposed for 0, 30, 60, 90 and 120 min to plant vitrification solution 2 (PVS2). As for the encapsulation-dehydration method, shoot tips were dried inside a laminar air flow cabinet for 0, 1, 2, 3, 4, 5 and 6 h at room temperature. The highest regrowth percentages were approximately 60 and 67 % for vitrification and encapsulation-dehydration, respectively. The best times were 60 min exposure to PVS2 for vitrification and 3 h desiccation for encapsulation-dehydration. Though these are preliminary results, the use of the cryopreservation techniques tested here proved to be an important asset in the conservation of this endangered species and will complement the conservation strategies previously developed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 12
• Opis fizyczny: p.3333-3336,fig.,ref.

Twórcy

autor

Coelho N.

• MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139, Faro, Portugal

autor

Gonzalez-Benito M.E.

• Departamento de Biología Vegetal, Universidad Politecnica de Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

autor

Romano A.

• MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139, Faro, Portugal

Bibliografia

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Identyfikatory

DOI

10.1007/s11738-014-1678-6