Clustering of Staphylococcus aureus bovine mastitis strains from regions of Central-Eastern Poland based on their biochemical and genetic characteristics

• Autorzy: Puacz E. , Ilczyszyn W.M. , Kosecka M. , Buda A. , Dudziak W. , Polakowska K. , Panz T. , Bialecka A. , Kasprowicz A. , Lisowski A. , Krukowski H. , Cuteri V. , Miedzobrodzki J.
• Języki publikacji: EN

Abstrakty

EN

Staphylococcus aureus strains were isolated from mastitic milk of cows with infected mammary glands. The animals were living in 12 different farms near Lublin, in Central-Eastern Poland. A biochemical identification method based on enzymatic assay was performed, followed by haemolytic and proteolytic tests. PCR-RFLP targeted on the gap gene allowed the genetic identification of strains at the species level and verified phenotypic identification results. A molecular typing method using triplex PCR was performed to recognize the genetic similarity of the analyzed strains. DNA microar-ray hybridization (StaphyType, Alere Technologies) was used for detection of antibiotic resistance and virulence associated markers. The results obtained indicate high genetic similarity in strains isolated from the same sites. High genetic similarities were also detected between strains isolated from cows from different farms of the same region. A slightly lower similarity was noted however, in strains from various regions indicating that the strains are herd specific and that the cow's infections caused by S. aureus were of a clonal character. In 21 representative isolates selected for DNA-microarray testing, only fosfomycin (fosB) and penicillin resistance markers (blaZ, blaI, blaR) were detected. The presence of genes coding for haemolysins (lukF, lukS, hlgA, hla, hld, hlb), proteases (aur, sspA, sspB, sspP), enterotoxins (entA, entD, entG, entI, entJ, entM, entN, entO, entR entU, egc-cluster), adhesins (icaA, icaC, icaD, bbp, clfA, clfB, fib, fnbA, map, vwb) or immune evasion proteins (scn, chp, sak) was common and, with exceptions, matched triplex PCR-defined clusters.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 2
• Opis fizyczny: p.333-342,fig.,ref.

Twórcy

autor

Puacz E.

• National Chamber of Medical Laboratory Specialists, Warsaw, Poland

autor

Ilczyszyn W.M.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Kosecka M.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Buda A.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Dudziak W.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Polakowska K.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Panz T.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

autor

Bialecka A.

• Center of Microbiology Research and Autovaccines, Krakow, Poland

autor

Kasprowicz A.

• Center of Microbiology Research and Autovaccines, Krakow, Poland

autor

Lisowski A.

• Faculty of Biology and Animal Breeding, University of Life Sciences, Lublin, Poland

autor

Krukowski H.

• Faculty of Biology and Animal Breeding, University of Life Sciences, Lublin, Poland

autor

Cuteri V.

• School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy

autor

Miedzobrodzki J.

• Faculty of Biochemistry, Biophysics and Biotechnology, Center, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland

Bibliografia

• Baum C, Haslinger-Loffler B, Westh H, Boye K, Peters G, Neumann C, Kahl BC (2009) Non-spatypeable clinical Staphylococcus aureus strains are naturally occurring protein A mutants. J Clin Microbiol 47: 3624-3629.
• Bukowski M, Władyka B, Dubin G (2010) Exfoliative toxins of Staphylococcus aureus. Toxins 2: 1148-1165.
• Camussone CM, Calvinho LF (2013) Virulence factors of Staphylococcus aureus associated with intramammary infections in cows: relevance and role as immunogens. Rev Argent Microbiol 45: 119-130.
• Castelani L, Santos AF, Dos Santos Miranda M, Zafalon LF, Pozzi CR, Arcaro JR (2013) Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows. Int J Mol Sci 14: 4326-4333.
• Chrobak D, Kizerwetter-Świda M, Rzewuska M, Moodley A, Guardabassi L, Binek M (2011) Molecular characterization of Staphylococcus pseudintermedius strains isolated from clinical samples of animal origin. Folia Microbiol (Praha) 56: 415-422.
• Cuteri V, Mezzasoma P, Valente C (2003) Application of biomolecular methods to Staphylococcus aureus strains from dairy cows. Vet Res Commun 27 (Suppl 1): 335-338.
• Feng Y, Chen CJ, Su LH, Hu S, Yu J, Chiu CH (2008) Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics. FEMS Microbiol Rev 32: 23-37.
• Fluit AC (2012) Livestock-associated Staphylococcus aureus. Clin Microbiol Infect 18: 735-744.
• Foster TJ, Geoghegan JA, Ganesh VK, Hoök M (2014) Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Micro-biol 12: 49-62.
• Karyński M, Sabat AJ, Empel J, Hryniewicz W (2008) Molecular surveillance of methicillin-resistant Staphylococcus aureus by multiple-locus variable number tandem repeat fingerprinting (formerly multiple-locus variable number tandem repeat analysis) and spa typing in a hierarchic approach. Diagn Microbiol Infect Dis 62: 255-262.
• Kot B, Piechota M, Wolska KM, Frankowska A, Zdunek E, Binek T, Kłopotowska E, Antosiewicz M (2012) Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk. Pol J Vet Sci 15: 677-683.
• Le Marechal C, Seyffert N, Jardin J, Hernandez D, Jan G, Rault L, Azevedo V, Francois P, Schrenzel J, van de Guchte M, Even S, Berkova N, Thiery R, Fitzgerald JR, Vautor E, Le Loir Y (2011) Molecular basis of virulence in Staphylococcus aureus mastitis. PloS One 6: e27354.
• Malinowski E, Gajewski Z (2010) Mastitis and fertility disorders in cows. Pol J Vet Sci 13: 555-560.
• Międzobrodzki J, Małachowa N, Markiewski T, Białecka A, Kasprowicz A (2008) Differentiation of Staphylococcus aureus isolates based on phenotypical characters. Postepy Hig. Med. Dosw. 62: 322-327.
• Monecke S, Slickers P, Ehricht R (2008) Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition. FEMS Immunol Med Microbiol 53: 237-251.
• Naidu AS, Andersson M, Międzobrodzki J, Forsgren A, Watts JL (1991) Bovine lactoferrin receptors in Staphylococcus aureus isolated from bovine mastitis. J Dairy Sci 74: 1218-1226.
• Nickerson SC (1993) Eliminating chronic Staphylococcus aureus mastitis. Vet Med 88: 375.
• Oliveira DC, de Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46: 2155-2161.
• Oliveira M, Bexiga R, Nunes SF, Vilela CL (2011) Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates. J Vet Sci 12: 95-97.
• Pejsak Z, Tarasiuk K (1989) The occurrence of endotoxin in sows with coliform mastitis. Theriogenology 32: 335-341.
• Peton V, Le Loir Y (2013) Staphylococcus aureus in veterinary medicine. Infect Genet Evol. 21: 602-615.
• Sabat A, Małachowa N, Międzobrodzki J, Hryniewicz W (2006) Comparison of PCR-based methods for typing Staphylococcus aureus isolates. J Clin Microbiol 44: 3804-3807.
• Saei HD, Ahmadi M, Mardani K, Batavani RA (2009) Molecular typing of Staphylococcus aureus isolated from bovine mastitis based on polymorphism of the coagulase gene in the north west of Iran. Vet Microbiol 137: 202-206.
• Szweda P, Schielmann M, Milewski S, Frankowska A, Jakubczak A (2012) Biofilm production and presence of ica and bap genes in Staphylococcus aureus strains isolated from cows with mastitis in the Eastern Poland. Pol J Microbiol 61: 65-69.
• Truszczyński MJ (1998) The role and importance of veterinary laboratories in the prevention and control of infectious diseases of animals. Rev Sci Tech 17: 405-410.
• Yugueros J, Temprano A, Sanchez M, Luengo JM, Naharro G (2001) Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene. J Clin Microbiol 39: 3693-3695.
• Zadoks R, Fitzpatrick J (2009) Changing trends in mastitis. Ir Vet J 62 (Suppl 4): S59-70.
• Zadoks RN, Middleton JR, McDougall S, Katholm J, Schukken YH (2011) Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. J Mammary Gland Biol Neoplasia 16: 357-372.
• Zdzalik M, Karim AY, Wolski K, Buda P, Wójcik K, Brueg-gemann S, Wojciechowski P, Eick S, Calander AM, Jonsson IM, Kubica M, Polakowska K, Międzobrodzki J, Władyka B, Potempa J, Dubin G (2012) Prevalence of genes encoding extracellular proteases in Staphylococcus aureus - important targets triggering immune response in vivo. FEMS Immunol Med Microbiol 66: 220-229.
• Zecconi A, Scali F (2013) Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases. Immunol Lett 150: 12-22.

Identyfikatory

DOI

10.1515/pjvs-2015-0043