Effects of cryopreservation at –80°C on the formulation and pathogenicity of the obligate aphid pathogen Pandora nouryi

• Autorzy: Zhou X. , Feng M.-G. , Huang Z.-H.
• Języki publikacji: EN

Abstrakty

EN

Cryopreservation at –80°C is an alternative to liquid nitrogen storage for Entomophthorales. However, detailed studies about its effects on fungal pathogenicity and formulation are very limited. In the present study, the obligate aphid pathogen Pandora nouryi was formulated as mycelia grown on millet-gel granules after preservation as primary spores at –80°C for 3–18 months, although its ability to produce infectious conidia gradually diminished. The sporulation capacity of this granular formulation was reduced to 18.5 × 104 conidia/mg after 18 months of storage, which was still higher than that of mycotized aphids. The half-decline time of sporulation capacity was computed as 13.6 months. The infectivity to the green peach aphid Myzus persicae had no significant decline in 12 months. The ability to yield resting spores within host carcasses remained unchanged, and the probability of resting spore formation increased with the conidial concentrations that infect aphids. Therefore, cryopreservation at –80°C exerted a marginal impact on formulation and pathogenicity of P. nouryi and can substitute for costly liquid nitrogen storage in routine laboratory studies. The potential of the formulation in aphid biocontrol can be maintained although there is a risk of losing fungal sporulation ability in long-term preservation.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2014
• Tom: 63
• Numer: 2
• Opis fizyczny: p.211-215,fig.,ref.

Twórcy

autor

Zhou X.

• National Joint Local Engineering Laboratory of Biopesticide high-efficient Preparation, School of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Lin’an 311300, People’s Republic of China

autor

Feng M.-G.

• Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China

autor

Huang Z.-H.

• College of Chemical Engineering, Huaqiao University, Xiamen 361021, People’s Republic of China

Bibliografia

• Castrillo L.A., L. Thomsen, P. Juneja and A.E. Hajek. 2007. Detection and quantification of Entomophaga maimaiga resting spores in forest soil using real-time PCR. Mycol. Res. 111: 324–331.
• Delalibera Jr. I. and R.A. Humber and A.E. Hajek. 2004. Preservation of in vitro cultures of the mite pathogenic fungus Neozygites tanajoae. Can. J. Microbiol. 50: 579–586.
• Feng M.G., C.L. Liu, J.H. Xu and Q. Xu. 1998. Modeling and biological implication of time-dose-mortality data for the Entomophthoralean fungus, Zoophthora anhuiensis, on the green peach aphid Myzus persicae. J. Invertebr. Pathol. 72: 246–251.
• Feng M.G. and Q. Xu. 2001. A simple method for routine maintenance and preservation of entomophthoraceous cultures. J. Invertebr. Pathol. 77: 141–143.
• Grundschober A., F.M. Freimoser, U. Tuor and M. Aebi. 2001.In vitro spore formation and completion of the asexual life cycle of Neozygites parvispora, an obligate biotrophic pathogen of thrips. Microbiol. Res. 156: 247–257.
• Grundschober A., U, Tuor and M. Aebi. 1998. In vitro cultivation and sporulation of Neozygites parvispora (Zygomycetes: Entomophthorales). Syst. Appl. Microbiol. 21: 461–469.
• Hajek A.E. and Jr. I. Delalibera. 2010. Fungal pathogens as classical biological control agents against arthropods. BioControl 55: 147–158.
• Hajek A.E., R.A. Humber and M.H. Griggs. 1990. Decline in virulence of Entomophaga maimaiga (Zygomycetes: Entomophthorales) with repeated in vitro subculture. J. Invertebr. Pathol. 56: 91–97.
• Hajek A.E., M. Shimazu and R.A. Humber. 1995. Instability in pathogenicity of Entomophaga maimaiga after long-term cryopreservation. Mycologia 87: 483–489.
• Hemmati F., J.K. Pell, H.A. McCartney, S.J. Clark and M.L. Deadman. 2001. Conidial discharge in the aphid pathogen Erynia neoaphidis. Mycol. Res. 105: 715–722.
• Huang Z.H. and M.G. Feng. 2008. Resting spore formation in vivo of obligate aphid pathogen Pandora nouryi depends on the inoculum concentration of host infection. Environ. Microbiol. 10: 1912–1916.
• Huang Z.H., M.G. Feng, X.X. Chen and S.S. Liu. 2008. Pathogenic fungi and parasitoids of aphids present in air captures of migratory alates in the low-latitude plateau of Yunnan, China. Environ. Entomol. 37: 1264–1271.
• Hubálek Z. 2003. Protectants used in the cryopreservation of microorganisms. Cryobiology 46: 205–229.
• Humber R.A. 1997. Fungi: preservation of cultures. In: L. Lacey (ed.), Manual of techniques in insect pathology. Academic Press, San Diego, pp. 269–279.
• Jensen A.B., L. Thomsen and J. Eilenberg. 2006. Value of host range, morphological, and genetic characteristics within the Entomophthora muscae species complex. Mycol. Res. 110: 941–950.
• Keller S. 2007. Fungal structures and biology. In: Keller S. (ed.), Arthropod-Pathogenic Entomophthorales: Biology, Ecology, Identification. Luxembourg: Office for Official Publications of the European Communities, pp. 27–34.
• Lόpez-Lastra C.C., A.E. Hajek and R.A. Humber. 2001. Effects of two cryopreservation techniques on viability and pathogenicity of entomophthoralean fungi. Can. J. Bot. 79: 861–864.
• Lόpez-Lastra C.C., A.E. Hajek and R.A. Humber. 2002. Comparing methods of preservation for cultures of entomopathogenic fungi. Can. J. Bot. 80:1126–1130.
• Papierok B. 2007. Part 2: isolating, growing and storing arthropod-pathogenic Entomophthorales. In: Keller S. (ed.), Arthropod-Pathogenic Entomophthorales: Biology, Ecology, Identification. Luxem-bourg: Office for Official Publications of the European Communities, pp. 66–81.
• Pell J.K., J. Eilenberg, A.E. Hajek and D.C. Steinkraus. 2001. Biology, ecology and pest management potential of Entomophthorales. In: Butt T.M., Jackson C., Magan N. (eds), Fungi as Biocontrol Agents. CABI Publishing, Wallingford, pp. 71–153.
• Pell J.K., J.J. Hannam and D.C. Steinkraus. 2010. Conservation biological control using fungal entomopathogens. BioControl 55: 187–198.
• Rohel E., Y. Couteaudier, B. Papierok, N. Cavelier and C.A. Dedry-ver. 1997. Ribosomal internal transcribed spacer size variation correlated with RAPD-PCR pattern polymorphisms in entomopathogenic fungus Erynia neoaphidis and some closely related species. Mycol. Res. 101: 573–579.
• Sierotzki H., F. Camastral, P.A. Shah, M. Aebi and U. Tuor. 2000. Biological characteristics of selected Erynia neoaphidis isolates. Mycol. Res. 104: 213–219.
• Sosa-Gómez D.R., C.C. López-Lastra and R.A. Humber. 2010. An overview of arthropod-associated fungi from Argentina and Brazil. Mycopathologia 170: 61–76.
• Tang Q.Y. and M.G. Feng. 2007. DPSData Processing System: Experimental Design, Statistical Analysis and Data Mining. Science Press, Beijing, China.
• Vingaard M.G., D.C. Steinkraus, G.O. Boys and J. Eilenberg. 2003. Effects of long-term storage at –14°C on the survival of Neozygites fresenii (Entomophthorales: Neozygitaceae) in cotton aphids (Homoptera: Aphididae). J. Invertebr. Pathol. 82: 97–102.
• Zhou X. and M.G. Feng. 2009. Sporulation, storage and infectivity of obligate aphid pathogen Pandora nouryi grown on novel granules of broomcorn millet and polymer gel. J. Appl. Microbiol. 107: 1847–1856.
• Zhou X. and M.G. Feng. 2010a. Biotic and abiotic regulation of resting spore formation in vivo of obligate aphid pathogen Pandora nouryi: modeling analysis and biological implication. J. Invertebr. Pathol. 103: 83–88.
• Zhou X. and M.G. Feng. 2010b. Improved sporulation of alginate pellets entrapping Pandora nouryi and millet powder and their potential to induce an aphid epizootic in field cages after release. Biol. Control. 54: 153–158.
• Zhou X., M.G. Feng and L.Q. Zhang. 2012. The role of temperature on in vivo resting spore formation of the aphid-specific pathogen Pandora nouryi (Zygomycota: Entomophthorales) under winter field conditions. Biocontrol. Sci. Techn. 22: 93–100.