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2013 | 35 | 09 |

Tytuł artykułu

The effect of sodium bicarbonate on plant performance and iron acquisition system of FA-5 (Forner-Alcaide 5) citrus seedlings

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This work studies the effect of bicarbonate on plant performance and the iron acquisition system of Forner-Alcaide 5 (FA-5) seedlings, a citrus genotype known for its tolerance to calcareous soils. Plants were irrigated for 6 weeks with or without 10 mM NaHCO3. Treatment significantly decreased shoot growth, photosynthetic levels and iron concentration in shoots and roots. o,o-57FeEDDHA experiments indicated that 57Fe uptake by roots was inhibited in treated plants. Moreover, those seedlings accumulated more 57Fe in roots, and enhanced mRNA accumulation of ferric reductase genes FRO1 and FRO2 and FC-R activity in roots. H+-ATPase activity and HA1 gene expression were also increased, while HA2 was not affected. In addition, expression of the iron transporter gene IRT1 was increased, while IRT2 was not significantly affected. Finally, according to PEPC enzymatic activity, PEPC1 gene expression was higher in treated roots. In conclusion, it appears that bicarbonate prevents medium acidification by roots, thus reducing Fe2+ uptake. Accordingly, Fe deficiency enhanced the expression of some genes related with the Fe acquisition system (IRT1, FRO1, FRO2, HA1 and PEPC1) and the activity of the corresponding enzymes, which appear to constitute an adaptive mechanism of FA-5 in these soils.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

09

Opis fizyczny

p.2833-2845,fig.,ref.

Twórcy

  • Department of Citriculture and Plant Production, Instituto Valenciano Investigaciones Agrarias, Crta Moncada-Na´quera, km 4.5, 46113 Valencia, Spain
  • Department of Citriculture and Plant Production, Instituto Valenciano Investigaciones Agrarias, Crta Moncada-Na´quera, km 4.5, 46113 Valencia, Spain
  • Department of Citriculture and Plant Production, Instituto Valenciano Investigaciones Agrarias, Crta Moncada-Na´quera, km 4.5, 46113 Valencia, Spain
  • Department of Citriculture and Plant Production, Instituto Valenciano Investigaciones Agrarias, Crta Moncada-Na´quera, km 4.5, 46113 Valencia, Spain
autor
  • Department of Citriculture and Plant Production, Instituto Valenciano Investigaciones Agrarias, Crta Moncada-Na´quera, km 4.5, 46113 Valencia, Spain

Bibliografia

  • Abadia J, Morales F, Abadia A (1999) Photosystem II efficiency in low chlorophyll, iron-deficient leaves. Plant Soil 215:183–192
  • Abadia J, López-Millán AF, Rombolá A, Abadia A (2002) Organic acids and Fe deficiency: a review. Plant Soil 241:75–86
  • Alcántara E, Romera FJ, Cañete M, de la Guardia MD (2000) Effects of bicarbonate and iron supply on Fe(III) reducing capacity of roots and leaf chlorosis of the susceptible peach rootstock Nemaguard. J Plant Nutr 23:1607–1617
  • Andaluz S, López-Milla´n AF, Peleato ML, Abadia J, Abadia A (2002) Increases in phosphoenolpyruvate carboxylase activity in irondeficient sugar beet roots: analysis of spatial localization and post-translational modification. Plant Soil 241:43–48
  • Andaluz S, Rodriguez-Celma J, Abadia A, Abadia J, López-Millán AF (2009) Time course induction of several key enzymes in Medicago truncatula roots in response to Fe deficiency. Plant Physiol Biochem 47:1082–1088
  • Bienfait HF, van den Briel W, Mesland-Mul NT (1985) Free space iron pools in roots. Plant Physiol 78:596–600
  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principles of protein-dye binding. Anal Biochem 72:248–254
  • Bustin SA (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29:23–39
  • Calatayud A, Ramirez JW, Iglesias DJ, Barreno E (2002) Effects of ozone on photosynthetic CO2 exchange, chlorophyll a fluorescence and antioxidant systems in lettuce leaves. Physiol Plant 116:308–316
  • Castle WS, Nunnallee J, Manthey JA (2009) Screening citrus rootstocks and related selections in soil and solution culture for tolerance to low-iron stress. HortScience 44:638–645
  • Chaney RL, Brown JC, Tiffin LO (1972) Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol 50:208–213
  • Chen Y, Barak P (1982) Iron nutrition of plants in calcareous soils. Adv Agron 35:217–240
  • Chouliaras V, Dimassi K, Therios I, Molassiotis A, Diamantidis G (2004a) Root reducing capacity, rhizosphere acidification, peroxidase and catalase activities and nutrient levels of Citrus taiwanica and Citrus volkameriana seedlings, under Fe deprivation conditions. Agronomie 24:1–6
  • Chouliaras V, Therios I, Molassiotis A, Diamantidis G (2004b) Iron chlorosis in grafted sweet orange (Citrus sinensis L.) plants: physiological and biochemical responses. Biol Plant 48:141–144
  • Cinnelli F (1995) Physiological responses of clonal quince rootstocks to iron-deficiency induced by addition of bicarbonate to nutrient solution. J Plant Nutr 18:77–89
  • Cohen CK, Norvell WA, Kochian LV (1997) Induction of the root cell plasma membrane ferric reductase. Plant Physiol 114:1061–1069
  • Connolly EL, Fett JP, Guerinot ML (2002) Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation. Plant Cell 14:1347–1357
  • Coulombe BA, Chaney RL, Wiebold WJ (1984) Bicarbonate directly induces iron chlorosis in susceptible soybean cultivars. Soil Sci Soc Am J 48:1297–1301
  • de la Guardia MD, Alcántara E (2002) A comparison of ferric chelate reductase and chlorophyll and growth ratios as indices of selection of quince, pear and olive genotypes under iron deficiency stress. Plant Soil 241:49–56
  • De Nisi P, Zocchi G (2000) Phosphoenolpyruvate carboxylase in cucumber (Cucumis sativus L.) roots under iron deficiency: activity and kinetic characterization. J Exp Bot 5:1903–1909
  • Deal GM, Alcántara E (2002) Bicarbonate and low iron level increase root to total plant weight ratio in Olive and Peach rootstock. J Plant Nutr 25:1021–1032
  • Dell'Orto M, Santi S, de Nisi P, Cesco S, Varanini Z, Zocchi G, Pinton R (2000) Development of Fe-deficiency responses in cucumber (Cucumis sativus L.) roots: involvement of plasma membrane H+-ATPase activity. J Exp Bot 51:695–701
  • Donnini S, Castagna A, Ranieri A, Zocchi G (2009) Differential responses in pear and quince genotypes induced by Fe deficiency and bicarbonate. J Plant Physiol 166:1181–1193
  • Eide DJ, Broderius M, Fett J, Guerinot ML (1996) A novel ironregulated metal transporter from plants identified by functional expression in yeast. Proc Natl Acad Sci USA 93:5624–5628
  • Fleming AL, Chaney RL, Coulombe BA (1984) Bicarbonate inhibits Fe-stress response and Fe uptake-translocation of chlorosissusceptible soybean cultivars. J Plant Nutr 7:699–714
  • Forner JB, Forner-Giner MA, Alcaide A (2003) Forner-Alcaide 5 and Forner-Alcaide 13: two new citrus rootstocks released in Spain. HortScience 38:629–630
  • Fox TC, Shaff JE, Grusak MA, Norvell WA, Chen Y, Chaney RL, Kochian LV (1996) Direct measurement of 59labeled Fe2+ influx in roots of Pisum sativum using a chelator buffer system to control Fe2+ in solution. Plant Physiol 111:93–100
  • Gharsalli M, Hajji M (2002) Comparison of physiological responses of peach and almond seedlings to iron deficiency. J Plant Nutr 25:1139–1154
  • González-Mas MC, Llosa MJ, Quijano A, Forner-Giner MA (2009) Rootstock effects on leaf photosynthesis in ‘Navelina’ trees grown in calcareous soil. HortScience 44:280–283
  • Goodstein DM, Shu S, Howson R, Neupane R, Hayes RD, Fazo J, Mitros T, Dirks W, Hellsten U, Putnam N, Rokhsar DS (2012) Phytozome: a comparative platform for green plant genomics. Nucleic Acids Res 40:1178–1186
  • Hashimoto JG, Beadles-Bohling AS, Wiren KM (2004) Comparison of RiboGreen and 18S rRNA quantitation for normalizing realtime RT-PCR expression analysis. Biotechniques 36:54–60
  • Jelali N, M0sehli W, Dell'orto M, Abdelly C, Gharsally M, Zocchi G (2010) Changes of metabolic responses to direct and induced Fe deficiency of two Pisum sativum cultivars. Environ Exp Bot 68:238–246
  • Jelali N, Salah IB, M0sehli W, Donnini S, Zocchi G, Gharsalli M (2011) Comparison of three pea cultivars (Pisum sativum) regarding their responses to direct and bicarbonate-induced iron deficiency. Sci Hortic 129:548–553
  • Jeong J, Connolly EL (2009) Iron uptake mechanisms in plants: functions of the FRO family of ferric reductases. Plant Sci 176:709–714
  • Kim SA, Guerinot ML (2007) Mining iron: iron uptake and transport in plants. FEBS Lett 581:2273–2280
  • Korcak RF (1987) Iron deficiency chlorosis. Hort Rev 9:133–186
  • Kosegarten H, Koyro HW (2001) Apoplastic accumulation of iron in the epidermis of maize (Zea mays) roots grown in calcareous soil. Physiol Plant 113:515–522
  • Ksouri R, Debez A, Mahmoudi H, Ouerghi Z, Gharsalli M, Lachaâl M (2007) Genotypic variability within Tunisian grapevine varieties (Vitis vinifera L.) facing bicarbonate-induced iron deficiency. Plant Physiol Biochem 45:315–322
  • Li LH, Cheng XD, Ling HQ (2004) Isolation and characterization of Fe(III) chelate reductase gene LeFRO1 in tomato. Plant Mol Biol 54:125–136
  • López-Millán AF, Morales F, Andaluz S, Gogorcena Y, Abadia A, de Las Rivas J, Abadia J (2000) Responses of sugar beet roots to iron deficiency. Changes in carbon assimilation and oxygen use. Plant Physiol 124:885–897
  • López-Millán AF, Morales F, Gogorcena Y, Abadia A, Abadia J (2009) Metabolic responses in iron deficient tomato plants. J Plant Physiol 166:375–384
  • Lucena C, Romera FJ, Rojas CL, Garcia MJ, Alcántara E, Pérez-Vicente R (2007) Bicarbonate blocks the expression of several genes involved in the physiological responses to Fe deficiency of Strategy I plants. Funct Plant Biol 34:1002–1009
  • Manthey JA, McCoy DL, Crowley DE (1994) Stimulation of rhizosphere iron reduction and uptake in response to iron deficiency in citrus rootstocks. Plant Physiol Biochem 32:211–215
  • Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London
  • Marschner H, Römheld V (1994) Strategies of plants for acquisition of iron. Plant Soil 165:261–274
  • Mengel K (1994) Iron availability in plant tissues—iron chlorosis on calcareous soils. Plant Soil 165:275–283
  • Mengel K, Breininger MT, Bubl W (1984) Bicarbonate, the most important factor inducing iron chlorosis in vine grapes on calcareous soil. Plant Soil 81:333–344
  • Mengel K, Planker R, Hoffmann B (1994) Relationship between leaf apoplast pH and iron chlorosis of sunflower (Helianthus annuus L.). J Plant Nutr 17:1053–1065
  • Molassiotis A, Tanou G, Diamantidis G, Patakas A, Therios I (2006) Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defence in two peach rootstocks differing in Fe deficiency tolerance. J Plant Physiol 163:176–185
  • Morales F, Abadia A, Abadia J (1991) Chlorophyll fluorescence and photon yield of oxygen evolution in iron-deficient sugar beet (Beta vulgaris L.) leaves. Plant Physiol 97:886–893
  • Moran R, Porath D (1980) Chlorophyll determination in intact tissues using N,N-dimethylformamide. Plant Physiol 65:478–479
  • Msilini N, Attia H, Bouraoui N, M'rah S, Ksouri R, Lachaâl M, Ouerghi Z (2009) Responses of Arabidopsis thaliana to bicarbonate-induced iron deficiency. Acta Physiol Plant 31:849–853
  • Nedunchezhian N, Morales F, Abadia A, Abadia J (1997) Decline in photosynthetic electron transport activity and changes in thylakoid protein pattern in field grown iron deficient peach (Prunus persica L.). Plant Sci 129:29–38
  • Pestana M, David M, de Varennes A, Abadia J, Araujo-Faria E (2001) Responses of ‘‘Newhall’’ orange trees to iron deficiency in hydroponics: effects on leaf chlorophyll, photosynthetic efficiency, and root ferric chelate reductase activity. J Plant Nutr 24:1609–1620
  • Pestana M, de Varennes A, Abadia J, Araujo-Faria E (2005) Differential tolerance to iron deficiency of citrus rootstocks grown in nutrient solution. Sci Hortic 104:25–36
  • Rabotti G, Zocchi G (1994) Plasma membrane-bound H+-ATPase and reductase activities in Fe-deficient cucumber roots. Physiol Plant 90:779–785
  • Rabotti G, de Nisi P, Zocchi G (1995) Metabolic implications in the biochemical responses to iron deficiency in cucumber (Cucumis sativus L.) roots. Plant Physiol 107:1195–1199
  • Robinson NJ, Procter CM, Connolly EL, Guerinot ML (1999) A ferric-chelate reductase for iron uptake from soils. Nature 397:694–697
  • Rombolá AD, Brüggemann W, López-Millán AF, Tagliavini M, Abadia J, Marangoni B, Moog PR (2002) Biochemical responses to iron deficiency in kiwifruit (Actinidia deliciosa). Tree Physiol 22:869–875
  • Romera FJ, Alcántara E, de la Guardia MD (1997) Influence of bicarbonate and metal ions on the development of Fe(III) reducing capacity by Fe-deficient cucumber (Cucumis sativus) plants. Physiol Plant 101:143–148
  • Santi S, Schmidt W (2009) Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots. New Phytol 183:1072–1084
  • Santi S, Cesco S, Varanini Z, Pinton R (2005) Two plasma membrane H+-ATPase genes are differentially expressed in iron-deficient cucumber plants. Plant Physiol Biochem 43:287–292
  • Susin S, Abadia A, González-Reyes JA, Lucena JJ, Abadia J (1996) The pH requirements for in vivo activity of the iron-deficiency ‘‘turbo’’ ferric chelate reductase. Plant Physiol 110:111–123
  • Treeby M, Uren N (1993) Iron deficiency stress responses amongst citrus rootstocks. Z Pflanz Bodenk 156:75–81
  • Vert GA, Grotz N, Dedaldechamp F, Gaymard F, Guerinot ML, Briat JF, Curie C (2002) IRT1 an Arabidopsis transporter essential for iron uptake from the soil and plant growth. Plant Cell 14:1223–1233
  • Waters BM, Blevins DG, Eide DJ (2002) Characterization of FRO1, a pea ferric-chelate reductase involved in root iron acquisition. Plant Physiol 129:85–94
  • Wegner LH, Zimmermann U (2004) Bicarbonate-induced alkalinization of the xylem sap in intact maize seedlings as measured in situ with a novel xylem pH probe. Plant Physiol 136:3469–3477
  • Weyer S, Schwieters JB (2003) High precision Fe isotope measurements with high mass resolution MC-ICPMS. Int J Mass Spectrom 226:355–368
  • White PF, Robson AD (1990) Response of lupins (Lupinus angustifolius L.) and peas (Pisum sativum L.) to Fe deficiency induced by low concentration of Fe in solution or by addition of HCO3 -. Plant Soil 125:39–47
  • Yan J, Yuan F, Long G, Qin L, Deng Z (2012) Selection of reference genes for quantitative real-time RT-PCR analysis in citrus. Mol Biol Rep 39:1831–1838
  • Yi Y, Guerinot ML (1996) Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency. Plant J 10:835–844
  • Zocchi G, Cocucci S (1990) Fe uptake mechanism in Fe-efficient cucumber roots. Plant Physiol 92:908–911
  • Zribi K, Gharsalli M (2002) Effect of bicarbonate on growth and iron nutrition of pea. J Plant Nutr 25:2143–2149
  • Zuo Y, Ren L, Zhang F, Jiang RF (2007) Bicarbonate concentration as affected by soil water content controls iron nutrition of peanut plants in calcareous soil. Plant Physiol Biochem 45:357–364

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