Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

• Autorzy: Yang K.L. , Lee J. T. , Pang C. J. , Lee T. Y. , Chen S. P. , Liew H. K. , Chen S. Y. , Chen T. Y. , Lin P. Y.
• Języki publikacji: EN

Abstrakty

EN

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Słowa kluczowe

EN

man; adipose-derived stem cell; stem cell; adult stem cell; treatment; intracerebral hemorrhage; rat; intravenous injection; neural stem cell; regenerative medicine; stem cell therapy; stroke; stromal cell

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2012
• Tom: 17
• Numer: 3
• Opis fizyczny: p.376-392, fig.,ref.

Twórcy

autor

Yang K.L.

• Buddhist Tzu Chi Stem Cells Center, Buddist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

autor

Lee J. T.

autor

Pang C. J.

autor

Lee T. Y.

autor

Chen S. P.

autor

Liew H. K.

autor

Chen S. Y.

autor

Chen T. Y.

autor

Lin P. Y.

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