Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D

Warianty tytułu

PL

Zmiany związane ze zdolnością do proliferacji i morfologią kalusa Nicotiana tabacum L. w odpowiedzi na różne stężenia 2,4-D

• Języki publikacji: EN

Abstrakty

EN

The common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the effectiveness of a synthetic auxin, 2,4-dichlorofenoxyacetic acid (2,4-D), instead of the generally applied natural auxin, indole-3-acetic acid (IAA), was tested for the proliferation of Nicotiana tabacum callus. The following concentrations of 2,4-D were tested: 0.1, 0.5, 1.0, 1.5, and 2.0 mg dm−3. Callus was derived from stem pith and its proliferation allowed on MS medium through five subcultures at 25°C and in darkness. After each passage, the fresh weight and morphological features of the callus were determined. The 0.5 mg dm−3 2,4-D treatment was the most favorable for producing the greatest increase in fresh weight in each of five subsequent subcultures as well as maintaining normal morphological features for proliferation. However, the 1.0 mg dm−3 2,4-D treatment in comparison with the lowest, 0.1 mg dm−3, was more beneficial when considering regular increases of fresh weight and a better cell cohesion for callus growth.

PL

Do indukcji i namnażania kalusa niezbędne jest zastosowanie odpowiedniej auksyny, podanej samodzielnie lub z cytokininą. Spośród naturalnych auksyn IAA jest często wykorzystywana do stymulowania proliferacji kalusa Nicotiana tabacum. Zastosowanie IAA wymaga wyższych stężeń ze względu na nietrwałość, wynikającą z wrażliwości na światło i wysoką temperaturę. Takich ograniczeń nie posiada syntetyczna auksyna 2,4-D. Celem tej pracy była ocena przydatności 2,4-D do proliferacji kalusa Nicotiana tabacum z następującymi wariantami stężeń 0.1, 0.5, 1.0, 1.5 i 2 mg dm−3. Proliferację kalusa z rdzenia łodygi przeprowadzono na pożywce MS przez 5 pasaży bez dostępu światła i w temperaturze 25°C. Po każdym pasażu określono świeżą masę i cechy morfologiczne kalusa takie jak barwa, struktura powierzchni i stopień spójności komórek. Spośród badanych stężeń 2,4-D, najefektywniej stymulującym było 0.5 mg dm−3 ze względu na najwyższy przyrost świeżej masy w każdym z pięciu kolejnych pasaży oraz sprzyjające proliferacji cechy morfologiczne kalusa. Wariant stężenia 1.0 mg dm−3 2,4-D w porównaniu z 0.1 mg dm−3 okazał się korzystniejszy, ponieważ charakteryzował się regularnymi przyrostami i lepszą dla namnażania spójnością komórek kalusa.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2017
• Tom: 70
• Numer: 4
• Opis fizyczny: Article 1725 [11p.], fig.,ref.

Twórcy

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Identyfikatory

DOI

10.5586/aa.1725