HPLC analysis of Amoxicillin using AccQ-fluor reagent for pre-column derivatization

• Autorzy: Kowalczuk D. , Galewska A.
• Języki publikacji: EN

Abstrakty

EN

A simple procedure to perform for the determination of amoxicillin is presented, based on reaction with 1-[(quinolin-6-ylcarbamoyl)oxy]pyrrolidine-2,5-dione (AccQ-Fluor) in borate buffer medium at pH of 7.4. The resulting amoxicillin+AccQ-Fluor derivative was determined by reversed-phase chromatography with combined fluorescence (λex=245 nm/λem=368 nm) and UV detection (λ=245 nm) for control. The calibration graph was linear (r=0.9992 ) over the range 5.0-30 μg/mL. The method was applied to the determination of amoxicillin in pharmaceutical formulations with acceptable precision and accuracy.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2012
• Tom: 21
• Numer: 1
• Opis fizyczny: p.139-143,fig.,ref.

Twórcy

autor

Kowalczuk D.

• Chair and Department of Medicinal Chemistry, Medical University of Lublin, Chodzki 6, 20-093 Lublin, Poland

autor

Galewska A.

Bibliografia

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