Immunocytochemical analysis of calretinin in the frontal cortex of chinchilla

• Autorzy: Krawczyk A. , Szalak R. , Jaworska-Adamu J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to define morphology and distribution of calretinin (CR) positive neurons in the frontal cortex of adult chinchilla males and intracellular localisation of the protein in this area. The brains of 5 adult chinchilla males were used in the study. CR immunoreactive neurons were shown with peroxidase-antiperoxidase immunohistochemical reaction using a specific monoclonal antibody. Intensive CR immunoreactivity was demonstrated mainly in few polymorphic neurons of II, III, and V layers. Cy toplasmic and nuclear reaction product in most CR positive neurons was diffuse and in some neurons of layer V in the form of granules localised peripherally. In a few cells more intensive staining was observed in the nuclei than in the cytoplasm. The results indicate the presence of heteromorphic CR positive neurons in specific lay ers of the frontal cortex. Nuclear localisation of CR in neurons suggests passive transport of this protein, which may affect the nuclear genes. This protein is a neuroprotector maintaining appropriate level of calcium, modulating neuronal activity, and synaptic conduction in the frontal cortex of chinchilla.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.103-107,fig.,ref.

Twórcy

autor

Krawczyk A.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland

autor

Szalak R.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland

autor

Jaworska-Adamu J.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland

Bibliografia

• 1. Aronica E., Redeker S., Boer K., Spliet W.G., Van Rijen P.C., Gorter J.A., Troost D.: Inhibitory networks in epilepsy-associated gangliogliomas and in the perilesional epileptic cortex. Epilepsy Res 2007, 74, 33-44.
• 2. Ballesteros Yanez I., Munoz A., Contreras J., Gonzalez J., Rodriguez-Veiga E., DeFelipe J.: Double bouquet cell in the human cerebral cortex and a comparison with other mammals. J Comp Neurol 2005, 486, 344-360.
• 3. Barinka F., Druga R.: Calretinin expression in the mammalian neocortex: a review. Physiol Res 2010, 59, 665-677.
• 4. Barinka F., Druga R., Marusic P., Krsek P., Zamecnik J.: Calretinin immunoreactivity in focal cortical dysplasias and in non-malformed epileptic cortex. Epilepsy Res 2010, 88, 76-86.
• 5. Beasley C.L., Zhang Z.J., Patten I., Reynolds G.P.: Selective deficits in prefrontal cortical GABAergic neurons in schizophrenia defined by the presence of calcium-binding proteins. Biol Psychiatry 2002, 52, 708- 715.
• 6. Billing-Marczak K., Kuznicki J.: Calretinin - sensor or buffer - function still unclear. Pol J Pharmacol 1999, 51, 173-178.
• 7. Cauli B., Tong X.K., Rancillac A., Serluca N., Lambolez B., Rossier J., Hamel E.: Cortical GABA interneurons in neurovascular coupling: relays for subcortical vasoactive pathways. J Neurosci 2004, 24, 8940-8949.
• 8. Clements R.J., McDonough J., Freeman E.J.: Distribution of parvalbumin and calretinin immunoreactive interneurons in motor cortex from multiple sclerosis postmortem tissue. Exp Brain Res 2008, 187, 459-465.
• 9. DeFelipe J.: Types of neurons, synaptic connections and chemical characteristics of cells immunoreactive for calbindin-D28K, parvalbumin and calretinin in the neocortex. J Chem Neuroanat 1997, 14, 1-19.
• 10. Desgent S., Boire D., Ptito M.: Distribution of calcium binding proteins in visual and auditory cortices of hamsters. Exp Brain Res 2005, 163, 159-172.
• 11. Druga R.: Neocortical inhibitory system. Folia Biol (Praha) 2009, 55, 201-217.
• 12. Elston G.N., Gonzalez-Albo M.C.: Parvalbumin-, calbindin-, and calretinin-immunoreactive neurons in the prefrontal cortex of the owl monkey (Aotus trivirgatus): a standardized quantitative comparison with sensory and motor areas. Brain Behav Evol 2003, 62, 19-30.
• 13. Fonseca M., Soriano E.: Calretinin-immunoreactive neurons in the normal human temporal cortex and in Alzheimer's disease. Brain Res 1995, 691, 83-91.
• 14. Gabbott P.L., Jays P.R., Bacon S.J.: Calretinin neurons in human medial prefrontal cortex (areas 24a,b,c, 32', and 25). J Comp Neurol 1997, 381, 389-410.
• 15. Glezer I.I., Hof P.R., Morgane P.J.: Calretinin immunoreactive neurons in the primary visual cortex of dolphin and human. Brain Res 1992, 595, 181-188.
• 16. Gonchar Y., Burkhalter A.: Three distinct families of GABAergic neurons in rat visual cortex. Cereb Cortex 1997, 7, 347-358.
• 17. Gonchar Y., Burkhalter A.: Distinct GABAergic targets of feedforward and feedback connections between lower and higher areas of rat visual cortex. J Neurosci 2003, 23, 10904-10912.
• 18. Hashimoto T., Volk D.W., Eggan S.M., Mirnics K., Pierri J.N., Sun Z., Sampson A.R., Lewis D.A.: Gene expression deficits in a subclass of GABA neurons in the prefrontal cortex of subjects with schizophrenia. J Neurosci 2003, 23, 6315-6326.
• 19. Hof P.R., Bogaert Y.E., Rosenthal R.E., Fiskum G.: Distribution of neuronal populations containing neurofilament protein and calcium- binding proteins in the canine neocortex: regional analysis and cell typology. J Chem Neuroanat 1996, 11, 81-98.
• 20. Hof PR., Nimchinsky E.A., Perl D.P., Erwin J.M.: An unusual population of pyramidal neurons in the anterior cingulate cortex of hominids contains the calcium-binding protein calretinin. Neurosci Lett 2001, 307, 139-142.
• 21. Hof P.R., Sherwood C.C.: Morphomolecular neuronal phenotypes in the neocortex reflect phylogenetic relationships among certain mammalian orders. Anat Rec Part A 2005, 287A, 1153-1163.
• 22. Jeon C.J., Park H.J.: Immunocytochemical localization of calcium-binding protein calretinin containing neurons in cat visual cortex. Mol Cells 1997, 7, 721-725.
• 23. Kawaguchi Y., Kubota Y.: GABAergic cell subtypes and their synaptic connections in rat frontal cortex. Cereb Cortex 1997, 7, 476-486.
• 24. Kawaguchi Y., Kondo S.: Parvalbumin, somatostatin and cholecystokinin as chemical markers for specific GABAergic interneuron types in the rat frontal cortex. J Neurocytol 2002, 31, 277-287.
• 25. Kowiański P.: Neuromodulators and calcium-binding proteins in maturation of the claustral neurons in the rat - immunocytochemical, morphometric and based on the retrograde axonal transport studies. Thesis, Akademia Medyczna, Gdansk, Poland, 2005.
• 26. Kubota Y., Hattori R., Yui Y.: Three distinct subpopulations of GABAergic neurons in rat frontal agranular cortex. Brain Res 1994, 649, 159-173.
• 27. Markram H., Toledo-Rodriguez M., Wang Y., Gupta A., Silberberg G., Wu C.: Interneurons of the neocortical inhibitory system. Neurosci 2004, 5, 793-807.
• 28. Park H.J., Lee S.N., Lim H.R., Kong J.H., Jeon C.J.: Calcium-binding proteins calbindin D28K, calretinin, and parvalbumin immunoreactivity in the rabbit visual cortex. Mol Cells 2000, 10, 206-212.
• 29. Schwaller B., Durussel I., Jennann D., Herrmann B., Cox J. A.: Comparison of the Ca²⁺ -binding properties of human recombinant calretinin-22k and calretinin. J Biol Chem 1997, 272, 29663-29671.
• 30. Sternberger L.A.: Immunocytochemistry, Ed. John Wiley and Sons, New York, USA, 1986.
• 31. Sucińska E.: Inhibitory neurotranssmitter in cerebral cortex plasticity. Kosmos 2005, 2-3, 195-212.
• 32. Tamamaki N., Yanagawa Y., Tomioka R., Miyazaki J., Obata K., Kaneko T.: Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse. J Comp Neurol 2003, 467, 60-79.
• 33. Zaitsev A.V., Gonzalez-Burgos G., Povysheva N.V., Kroner S., Lewis D.A., Krimer L.S.: Localization of calcium-binding proteins in physiologically and morphologically characterized interneurons of monkey dorsolateral prefrontal cortex. Cereb Cortex 2005, 15, 1178-1186.
• 34. Zaitsev A.V., Povysheva N.V., Gonzalez-Burgos G., Rotaru D., Fish K.N., Krimer L.S., Lewis D.A.: Interneuron diversity in layers 2-3 of monkey prefrontal cortex. Cereb Cortex 2009, 19, 1597-1615.