Effect of vasoactive intestinal peptide (VIP) on cytokine levels and haemostatic and biochemical parameters in a rat endotoxaemic model

• Autorzy: Col R. , Durgun Z.
• Języki publikacji: EN

Abstrakty

EN

The presented study was planned to determine whether vasoactive intestinal peptide (VIP) could prevent cytokine haemostatic, haematological, and biochemical disturbances in LPS-treated rats. Adult male Wistar rats (weight range: 200-250 g) were used. The study included four groups: group 1 served as a control group (C); animals in group 2 were given intravenously 1.6 mg/100 g of LPS (E. coli, serotype 0.111 :B4); in group 3, rats were injected intraperitoneally with 25 ng/kg of VIP; in group 4, the same doses of VIP and LPS were injected simultaneously. Blood samples were collected 6 h after treatments. In endotoxaemic rats, platelet count, fibrinogen, and antithrombin levels were decreased, the activated partial thromboplastin time and prothrombin time were prolonged, and leucopoenia, as well as significant changes in differential leukocyte percentage were demonstrated. In addition, LPS caused statistically significant increases in plasma TNF-α, IL-6, and IL-10 levels, and AST, ALT, creatinine, cholesterol, triglyceride concentrations. However, it caused a statistically significant decrease in total protein and albumin levels when compared to control group. The results showed that during endotoxaemia, VIP had moderately therapeutic effect as an antiinflammatory agent, suppressing TNF-α and IL-6, and stimulating IL-10; however, it was not effective against the adverse effect of LPS on investigated haematological and biochemical parameters.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.63-67,ref.

Twórcy

autor

Col R.

• Department of Physiology,Selcuk University, 42 075 Konya, Turkey

autor

Durgun Z.

Bibliografia

• 1. Al-Dughaym A.M.: Some endotoxin-induced clinical and biochemical changes in plasma of camels (Camelus dromedaricus). Vet Res Commun 2004, 28, 711 -718.
• 2. Allam G.: Vasoactive intestinal peptide inhibits liver pathology in acute murine Schistosomiasis mansoni and modulates IL-10, IL-12 and TNF-1 production. Immunobiology 2007, 212, 603-612.
• 3. Asakura H., Asamura R., Ontachi Y., Hayashi T., Yamazaki M., Morishita E., Miyamoto K.I., Nakao S.: Selective inducible nitric oxide synthase inhibition attenuates organ dysfunction and elevated endothelin levels in LPS-induced DIC model rats. J Thromb Haemost 2005, 3, 1050-1055.
• 4. Col R., Keskin E., Atalay B.: Effect of pentoxifylline on endotoxin-induced haemostatic disturbances in rabbits. Acta Vet Hung 2005, 53, 325-335.
• 5. Delgado M., Martinez C., Pozo D., Calvo J.R., Leceta J., Ganea D., Gomariz R.P.: Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activation polypeptide (PACAP) protect mice from lethal endotoxemia through the inhibition of TNF-α and IL-6. J Immunol 1999, 162, 1200-1205.
• 6. Delgado M., Pozo D., Martinez C., Leceta J., Calvo J.R., Ganea D., Gomariz R.P.: Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibit endotoxin-induced TNF-a production by macrophages, in vitro and in vivo studies. J Immunol 1999, 162, 2358-2367.
• 7. Dhuley J.N., Naik S.R.: Effect of rhinax on bacterial lipopolysaccharide induced endotoxemia in rats. Indian J 21. Exp Biol 1998, 36, 315-317.
• 8. Fernández-Martínez E., Morales-Ríos M.S., Pérez- Alvarez V., Muriel P.: Immunomodulatory effects of thalidomide analogs on LPS-induced plasma and hepatic cytokines in the rat. Biochem Pharmacol 2004, 68, 1321-1329.
• 9. Fourrier F., Chopin C., Huart J.J., Runge I., Caron C., Goudemand J.: Double-blind placebo-controlled trial of antithrombin III concentrate in septic shock with disseminated intravascular coagulation. Chest 1993, 104, 24. 882-892.
• 10. Fraunberg P., Schaefer S., Werdan K., Walli A.K., Seidel D.: Reduction of circulating cholesterol and apoliloprotein levels during sepsis. Clin Chem Lab Med 1999, 37, 357-362.
• 11. Gavina J.M., Dale D.C., Root R.K.: Neutrophils: function and role in sepsis syndrome. Sepsis 1998, 2, 107-117.
• 12. Gonzalez-Rey E., Delgado M.: Vasoactive intestinal peptide and regulatory T-cell induction: a new mechanism and therapeutic potential for immune homeostasis. Trends Mol Med 2007, 13, 241-251.
• 13. Grimm M.C., Newman R., Hassini Z., Cuan N., Connor S.J., Le Y., Wang J.M., Oppenheim J.J., Lloyd A.R.: Cutting edge: vasoactive intestinal peptide acts as a potent suppressor of inflammation in vivo by transdeactivating chemokine receptors. J Immunol 2003, 171, 4990-4994.
• 14. Hayashi T., Kishiwada M., Fujii K., Yuasa H., Nishioka J., Ido M., Gabazza E.C., Suzuki K.: Lipopolysaccharide-induced decreased protein S expression in liver cells is mediated by MEK/ERK signaling and NFjB activation: involvement of membrane-bound CD14 and toll-like receptor-4. J Thromb Haemost 2006, 4, 1763-1773.
• 15. Hopper K., Bateman S.: An updated view of hemostasis: mechanisms of hemostatic dysfuntion associated with sepsis. J Vet Emerg Crit Care 2005, 15, 83-91.
• 16. Iba T., Nakarai E., Takayama T., Nakajima K., Sasaoka T., Ohno Y.: Combination effect of antithrombin and recombinant human soluble thrombomodulin in a lipopolysaccharide induced rat sepsis model. Crit Care 2009, 13, R203.
• 17. Izutani W., Fujita M., Nishizawa K., Koga J.: Urinary protein C inhibitor as a therapeutic agent to disseminated intravascular coagulation (DIC): A comparison with low molecular weight heparin in rats with lipopolysaccharide-induced DIC. Biol Pharm Bull 2000, 23, 1046-1050.
• 18. Lauw F.N., Pajkrt D., Hack C.E., Kurimoto M., van Deventer S.J.H., van der Poll T.: Proinflammatory effects of IL-10 during human endotoxemia. J Immunol 165, 2783-2789.
• 19. Levi M., de Jonge E., van der Poll T.: Rationale for restoration of physiological anticoagulant pathways in patients with sepsis and disseminated intravascular coagulation. Crit Care Med 2001, 29, 90-94.
• 20. Mathiak G., Grass G., Herzmann T., Luebke T., Zetina C.C., Boehm S.A., Bohlen H., Neville L.F., Hoelscher A.H.: Caspase-1-inhibitor ac-YVAD-cmk reduces LPS- lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol 2000, 131, 383-386.
• 21. Moller S., Laigaard F., Olgaard K., Hemmingsen C.: Effect of 1,25-dihydroxy-vitamin D3 in experimental sepsis. Int J Med Sci 2007, 4, 190-195.
• 22. Pozo D., Guerrero J.M., Calvo J.R.: Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibit LPS-stimulated MIP-1α production and mRNA expression. Cytokine 2002, 18, 35-42.
• 23. Said S.I.: Vasoactive intestinal peptide and nitric oxide: divergent roles in relation to tissue injury. Ann NY Acad Sci 1996, 805, 379-387.
• 24. Saito K., Yamatani K., Manaka H., Takahashi K., Tominaga M., Sasaki H.: Role of Ca²⁺ on vasoactive intestinal peptide-induced glucose and adenosine 3',5'- monophosphate production in the isolated perfused rat liver. Endocrinology 1992, 130, 2267-2273.
• 25. Tuncel N., Tore F., Sahinturk V., Ak D., Tuncel M.: Vasoactive intestinal peptide inhibits degranulation and changes granular content of mast cells: a potential therapeutic strategy in controlling septic shock. Peptides 2000, 21, 81-89.
• 26. van Devender S.J.H., Buller H.R., ten Cate J.W., Aareden L.A., Hack C.E., Sturk A.: Experimental endotoxemia in humans; analysis of cytokine release and coagulation, fibrinolytic, and complement pathways. Blood 1990, 76, 2520-2526.
• 27. van Veen S.Q., Cheung C.W., Meijers J.C.M., van Gulik T.M., Boermeester M.A.: Anticoagulant and anti-inflammatory effects after peritoneal lavage with antithrombin in experimental polymicrobial peritonitis. J Thromb Haemost 2006, 4, 2343-2351.
• 28. van Westerloo D.J., Giebelen I.A.J., Meijers J.C.M., Daalhuisen J., de Vos A.F., Levi M., van der Poll T.: Vagus nerve stimulation inhibits activation of coagulation and fibrinolysis during endotoxemia in rats. J Thromb Haemost 2006, 4, 1997-2002.
• 29. Yamazaki M., Aoshima K., Mizutani T., Ontachi Y., Saito M., Morishita E., Asakura H., Matsuda T., Triplett D.A.: Prednisolone inhibits endotoxin-induced disseminated intravascular coagulation and improves mortality in rats: importance of inflammatory cytokine suppression Blood Coag Fibrinol 1999, 10, 321-330.
• 30. Yang H., Li Q.W., Han Z.S., Hu J.H., Li W.Y., Liu Z.B.: Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model. J Thromb Thrombolysis 2009, 28, 449-457.
• 31. Yazar E., Col R., Konyalıoglu S., Birdane Y.O., Elmas M., Bas A.L.: Effects of vitamin E and prednisolone on biochemical and haematological parameters in endotoxaemic New Zealand white rabbits. Bull Vet Inst Pulawy , 2004, 48, 105-108.
• 32. Yun-Choi H.S., Pyo M.K., Chang K.C., Lee D.H.: The effects of higenamine on LPS-induced experimental disseminated intravascular coagulation (DIC) in rats. Planta Med 2002, 68, 326-329.