The effect of low-dose experimental zearalenone intoxication on the immunoexpression of estrogen receptors in the ovaries of pre-pubertal bitches

• Autorzy: Gajecka M.
• Języki publikacji: EN

Abstrakty

EN

Zearalenone is an estrogenic mycotoxin that often contaminates plant material used in the production of feeds for companion animals. Small daily doses of ingested zearalenone - a competitive substrate modulating the activity of enzymes participating in estrogen biosynthesis at the pre-receptor level - can induce subclinical symptoms of hyperestrogenism in bitches. The objective of this study was to determine the effects of low zearalenone doses on the presence of estrogen receptors in the ovaries of pre-pubertal Beagle bitches. The bitches were divided into three groups of 10 animals each: experimental group I - 50 μg zearalenone/kg body weight administered once daily per os; experimental group II - 75 μg zearalenone/kg body weight administered once daily per os; control group - placebo containing no ZEN administered per os. The animals were ovariorectomized at the end of the experiment, at 112 days of age. Estrogen receptors were detected in ovarian specimens by immunohistochemical methods. The results revealed an absence of estrogen receptors alpha in all groups. In both experimental groups a decrease in the positive response of estrogen receptors beta in specified structures of ovaries was observed. Very low α-zearalenol levels probably attested to the slowing down (hypostimulation) of the biotransformation process. Overall, zearalenone intoxication led to hyperestrogenism during a specific developmental stage of pre-pubertal bitches. As regards hormesis, the threshold dose of zearalenone (adaptive capability) was exceeded in the ovaries of experimental group II animals. The results obtained in both experimental groups suggest that long-term exposure to low-dose zearalenone intoxication decreased the degree of estrogen receptors beta staining in particular structures of ovaries in the experimental bitches, which initiated epigenetic modification mechanisms that inhibited ovarian development.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 4
• Opis fizyczny: p.685-691,fig.,ref.

Twórcy

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

• Benassayag C, Perrot-Applanat M, Ferre F (2002) Phytoestrogens as modulators of steroid action in target cells. J Chromatogr B 777: 233-248.
• Boermans HJ, Leung MC (2007) Mycotoxins and the pet food industry: toxicological evidence and risk assessment. Int J Food Microbiol 119: 95-102.
• Caloni F, Cortinovis C (2010) Effects of fusariotoxins in the equine species. Vet J 186: 157-161.
• da Silva Faria T, de Bittencourt Brasil F, Sampaio FJ, da Fonte Ramos C (2008) Maternal malnutrition during lactation alters the folliculogenesis and gonadotropins and estrogen isoforms ovarian receptors in the offspring at puberty. J Endocrinol 198: 625-634.
• De Bosschere H, Ducatelle R, Tshamala M, Coryn M (2002) Changes in sex hormone receptors during administration of progesterone to prevent estrus in the bitch. Theriogenology 58: 1209-1217.
• Gajęcka M, Jakimiuk E, Polak M, Otrocka-Domagała I, Janowski T, Zwierzchowski W, Obremski K, Zielonka Ł, Apoznański J, Gajęcki M (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
• Gajęcka M, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2009) The biotransformation of chosen mycotoxins. Pol J Vet Sci 12: 293-303.
• Gajęcka M, Janowski T, Jakimiuk E, Polak M, Podhalicz-Dzięgielewska M, Rotkiewicz T, Otrocka-Domagała I, Obremski K, Zielonka Ł, Gajęcki M (2007) Histopathological and immunohistochemical examinations and changes in proliferation activity of the uterus in bitches following zearalenon mycotoxicosis. Pol J Vet Sci 10: 143-151.
• Gajęcka M, Rybarczyk L, Zwierzchowski W, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2011a) The effect of experimental, long-term exposure to low-dose zearalenone mycotoxicosis on the histological condition of ovaries in sexually immature gilts. Theriogenology 75: 1085-1094.
• Gajęcka M, Woźny M, Brzuzan P, Zielonka Ł, Gajęcki M (2011b) Expression of CYPscc and 3β-HSD mRNA in bitch ovary after long-term exposure to zearalenone. Bull Vet Inst Pulawy 55: 777-780.
• Gajęcki M, Gajęcka M, Jakimiuk E, Zielonka Ł, Obremski K (2010) Zearalenone – undesirable substance. In: Rai M, Varma A (eds) Mycotoxins in food, feed and bioweapons. Springer-Verlag, Berlin Heidelberg, pp 131-144.
• Grundy SA, Feldman E, Davidson A (2002) Evaluation of Infertility in the Bitch. Clin Tech Small Anim Practice. 17: 108-115.
• Knapczyk K, Duda M, Durlej M, Galas J, Koziorowski M, Slomczynska M (2008) Expression of estrogen receptor α (ERα) and estrogen receptor β (ERβ) in the ovarian follicles and corpora lutea of pregnant swine. Domest Anim Endocrinol 35: 170-179.
• Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, van der Saag PT, van der Burg B, Gustafsson JA (1998) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology 139: 4252-4263.
• Leader JE, Wang C, Popov VM, Fu M, Pestell RG (2006) Epigenetics and the estrogen receptor. Ann NY Acad Sci 1089: 73-87.
• Liu Y, Gao H, Marstrand TT, Strom A, Valen E, Sandelin A, Gustafsson JA, Dahlman-Wright K (2008) The genome landscape of ERα- and ERβ-binding DNA regions. Proc Natl Acad Sci USA 105: 2604-2609.
• Mayr U, Butsch A, Schneider S (1992) Validation of two in vitro test systems for estrogenic activities with zearalenone, phytoestrogens and cereal extracts. Toxicology 74:135-149.
• McDonnell DP, Connor CE, Wijayaratne A, Chang CY, Norris JD (2002) Definition of the molecular and cellular mechanisms underlying the tissue-selective agonist/antagonist activities of selective estrogen receptor modulators. Recent Prog Horm Res 57: 295-316.
• Morgavi DP, Riley RT (2007) An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with Fusarium toxins. Anim Feed Sci Tech 137: 201-212
• Mueller SO, Simon S, Chae K, Metzler M, Korach KS (2004) Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor a (ERa) and ERβ in human cells. Toxicol Sci 80: 14-25.
• Plewka A (2011) The metabolism of xenobiotics in alimentary system with special regard to the role of large intestine. Proceedings, VII International Scientific Conference: Veterinary Feed Hygiene – The Effects of Mycotoxins on Gastrointestinal Function. 23-24 September 2011, Olsztyn, Poland, pp 60-69.
• Shang Y, Brown M (2002) Molecular determinants for the tissue specificity of SERMs. Science 295: 2465-2468.
• Singh MN, Stringfellow HF, Paraskevaidis E, Martin-Hirsch PL, Martin FL (2007) Tamoxifen: important considerations of a multi-functional compound with organ-specific properties. Cancer Treat Rev 33: 91-100.
• Słomczyńska M (2002) The dynamic of the steroid hormone receptors distribution in the ovary. Post Biol Kom 29: 27-46.
• Taylor SE, Martin-Hirsch P.L, Martin FL (2010) Oestrogen receptor splice variants in the pathogenesis of disease. Cancer Lett 288: 133-148.
• Tiemann U, Tomek W, Schneider F, Vanselow J (2003) Effects of the micotoxins α- and β-zearalenol on regulation of progesterone synthesis in cultured granulosa cells from porcine ovaries. Reprod Toxicol 17: 673-681.
• Ververidis HN, Boscos CM, Stefanakis A, Saratsis P, Stamou AI, Krambovitis E (2004) Serum estradiol-17β, progesterone and respective uterine cytosol receptor concentrations in bitches with spontaneous pyometra. Theriogenology 62: 614-623.
• Visconti A, Pascale M (1998) Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. J Chromatogr A 815: 133-140.
• Watson CS, Alyea RA, Jeng Y-J, Kochukov MY (2007) Nongenomic actions of low concentration estrogens and xenoestrogens on multiple tissues. Mol Cell Endocrinol 274:1-7.
• Wąsowicz K, Chmielewska M, Łosiewicz K (2011) Estrogen receptors – morphology, physiology, pathology. Proceeding, VII International Scientific Conference: Veterinary Feed Hygiene – The Effects of Mycotoxins on Gastrointestinal Function. 23-24 September 2011, Olsztyn, Poland, pp 56-59.
• Wuttke W, Jarry H, Westphalen S, Christoffel V, Seidlova-Wuttke D (2002) Phytoestrogens for hormone replacement therapy? J Steroid Biochem Mol Biol 83: 133-147.
• Zanghf A, Catone G, Marino G, Quartuccio M, Nicntina PA (2007) Endometrial polypoid adenomyomatosis in a bitch with ovarian granulosa cell tumour and pyometra. J Comp Pathol 136: 83-86.
• Zwierzchowski W, Gajęcki M, Obremski K, Zielonka Ł, Baranowski M (2004) The occurrence of zearalenone and its derivatives in standard and therapeutic feeds for companion animals. Pol J Vet Sci 7: 289-293

Uwagi

rekord w opracowaniu