Contamination of foetal bovine serum with bovine viral diarrhoea virus (BVDV)

• Autorzy: Polak M.P. , Rola J. , Zmudzinski J. F.
• Języki publikacji: EN

Abstrakty

EN

Twelve batches of commercially available foetal bovine sera were tested for the presence of bovine viral diarrhoea virus (BVDV) and BDVD antibodies. The antibodies were found in three batches coming from two suppliers. Fragments of BVDV genome were present in five of these batches when tested by RT-PCR. All of them were type 1 BVDV according to a direct sequencing of PCR products. However, the further analysis of the samples by virus isolation showed that all RT-PCR positive batches contained non-infectious BVDV. Hence, inactivation methods used during the production process proved to be efficient making the tested sera safe for the use in cell culture and for vaccine production. Results of RT-PCR detection of viral genome must be treated with caution since not in every case positive results indicate that the virus is live and infectious. Genetic typing based on sequence analysis of PCR products confirmed wide geographic spread of donor cattle.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.501-505,fig.,ref.

Twórcy

autor

Polak M.P.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rola J.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzinski J. F.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Audet S.A., Crim R.L., Beeler J.: Evaluation of vaccines, interferons and cell substrates for pestivirus contamination. Biologicals 2000, 28, 41-46.
• 2. Bolin S.R., Matthews P.J., Ridpath J.F.: Methods for detection and frequency of contamination of fetal calf serum with bovine viral diarrhea virus and antibodies against bovine viral diarrhea virus. J Vet Diagn Invest 1991, 3, 199-203.
• 3. Bolin S.R., Ridpath J.F.: Prevalence of bovine viral diarrhea virus genotypes and antibody against those viral genotypes in fetal bovine serum. J Vet Diagn Invest 1998, 10, 135-139.
• 4. Falcone E., Tollis M., Conti G.: Bovine viral diarrhea disease associated with a contaminated vaccine. Vaccine 2000, 18, 387-388.
• 5. Giangaspero M., Vacirca G., Harasawa R., Büttner M., Panuccio A., De Giuli Morghen C., Zanetti A., Belloli A., Verhuist A.: Genotypes of pestivirus RNA detected in live virus vaccines for human use. J Vet Med Sci 2001, 63, 723-733.
• 6. Gilbert S.A., Burton K.M., Prins S.E., Deregt D.: Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR. J Clin Microbiol 1999, 37, 2020-2023.
• 7. Harpin S., Mehdy Elahi S., Cornaglia E., Yolken R.H., Elazhary Y.: The 5'-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus. Arch Virol 1995, 140, 1285-1290.
• 8. Hurtado A., Garcia-Perez A.L., Aduriz G., Juste R.A.: Genetic diversity of ruminant pestiviruses from Spain. Virus Res 2003, 92, 67-73.
• 9. Makoschey B., van Gelder P.T., Keijsers V., Goovaerts D.: Bovine viral diarrhoea virus antigen in foetal calf serum batches and consequences of such contamination for vaccine production. Biologicals 2003, 31, 203-208.
• 10. Sakoda Y., Ozawa S., Damrongwatanapokin S., Sato M., Ishikawa K., Fukusho A.: Genetic heterogeneity of porcine and ruminant pestiviruses mainly isolated in Japan. Vet Microbiol 1999, 65, 75-86.
• 11. Studer E., Bertoni G., Candrian U.: Detection and characterization of pestivirus contaminations in human live viral vaccines. Biologicals 2002, 30, 289-296.
• 12. Vilcek S., Paton D.J., Durkovic B., Strojny L., Ibata G., Moussa A., Loitsch A., Rossmanith W., Vega S., Scicluna M.T., Palfi V.: Bovine viral diarrhea virus genotype 1 can be separated into at least eleven genetic groups. Arch Virol 2001, 146, 99-115.
• 13. Yamamoto T., Kozasa T., Aoki H., Sekiguchi H., Morino S., Nakamura S.: Genomic analyses of bovine viral diarrhea viruses isolated from cattle imported into Japan between 1991 and 2005. Vet Microbiol 2008, 127, 386-391.