Genetic integrity of ex situ genebank collections

• Autorzy: Chebotar S. , Roder M. S. , Korzun V. , Borner A.
• Języki publikacji: EN

Abstrakty

EN

The genetic integrity of four accessions of the cross-pollinating species rye (Secale cereale L.) was investigated. Seeds available from the first and most recent regeneration cycles, multiplied 8, 12 (twice) or 14 times were fingerprinted using microsatellite markers. In all four accessions the allele numbers and frequencies changed after regeneration. Alleles present in the original seed sample were not detectable in the regenerated populations, whereas on the other hand, alleles were found in the recent seed sample, which were not observed in the investigated plants of the original one.

Słowa kluczowe

EN

genetic integrity; gene bank collection; rye; Secale cereale; cross-pollination; maintenance; fingerprinting; molecular marker

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2A
• Opis fizyczny: p.437-444,fig.,ref.

Twórcy

autor

Chebotar S.

• Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr.3, D-06466 Gatersleben, Germany

autor

Roder M. S.

• Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr.3, D-06466 Gatersleben, Germany

autor

Korzun V.

• Lochow-Petkus GmbH, PF 1197, D-29296 Bergen, Germany

autor

Borner A.

• Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr.3, D-06466 Gatersleben, Germany

Bibliografia

• 1. Borner, A., Chebotar, S. and Korzun, V. Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long term maintenance. Theor. Appl. Genet. 100 (2000) 494-497.
• 2. Perez De La Vega, M. and Allard, R. W. V. Mating system and genetic polymorphism in populations of Secale cereale and S. vavilovii. Can. J. Genet. Cytol. 26 (1984) 308-317.
• 3. Ramirez, L., Pisabarro, G. and Perez De La Vega, M. Izozyme genetic similarity among rye (Secale cereale L.) cultivars. J. Agric. Sci. 74 (1985) 495-500.
• 4. Adam, D., Simonsen, V. and Loeschcke, V. Allozyme variation in rye, Secale cereale L. 2. Commercial varieties. Theor. Appl. Genet. 74 (1987) 560-565.
• 5. Carnide, V., Pinto-Carnide, O., Matos, M., Guedes-Pinto, H. and Benito, C. Morphological and yield components and isozyme characterization of Portuguese rye populations. J. App. Genet. 38B ( 1997) 299-304.
• 6. Matos, M., Pinto-Carnide, O., Benito, C. Phylogenetic relationships among Portuguese rye based on isozyme, RAPD and ISSR markers. Hereditas L4 (2001) 229-236.
• 7. Persson, K., Diaz, O. and Von Bothmer, R. Extent and patterns of RAPD variation and landraces and cultivars of rye (Secale cereale L.) from Northern Europe. Hereditas 134 (2001) 237-243.
• 8. Garza, J . C. and Williamson, E. G. Detection of reduction in population size using data from microsatellite loci. Mol. Ecology 10 (2001) 305-318.
• 9. Mahmut, H., Ganzorig, S., Onuma, M., Masuda, R., Masatsugu, S. and Ohtaishi, N. A preliminary study of the genetic diversity of Xinjiang Tarim red deer (Cervus elaphus yarkandensis) using the microsatellite DNA method. Jpn. J. Vet. Res. 49 (2001) 231-237.
• 10. Röder, M. S., Plaschke, J., König, S. U., Börner, A., Sorrells, M. E., Tanksley, S. D. and Ganal, M.W. Abundance, variability and chromosomal location of microsatellites in wheat. Mol. Gen. Genet. 246 (1995) 327-333.
• 11. Röder, M. S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M. -H., Leroy, P. and Ganal, M. W. A microsatellite map of wheat. Genetics 149 (1998) 2007-2023.
• 12. Dallas, J. F. Estimation of microsatellite mutation rates in recombination inbred strains of mouse. Mamm. Genome 5 (1992) 32-38.
• 13. Payseur, B. A. and Nachman, M. W. Microsatellite variation and recombination rate in the human genome. Genetics 156 (2000) 1285-1298.