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2013 | 35 | 01 |

Tytuł artykułu

Identification and expression analysis of key enzymes of the terpenoids biosynthesis pathway of a liverwort Plagiochasma appendiculatum by EST analysis

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Liverworts are known to be a rich source of terpenoids and phenolic compounds whose biochemical and molecular biosynthesis properties are not well understood. To evaluate the biosynthetic processes of such compounds found in a liverwort Plagiochasma appendiculatum, a total of 5,024 clones were sequenced from a normalized cDNA library from leaves of P. appendiculatum. This produced 4,384 high-quality ESTs with a mean length of 550 bp. Cluster analysis indicated the presence of 704 contigs and 2,720 singletons, generating 3,424 unique sequences. A total of 1,180 sequences were functionally classified using gene ontologies (GO). Based on the homology to sequences present in GenBank, our EST collection was found to contain orthologs for known prenyl transferases and for genes involved in the 2-C-methyl-Derythritol 4-phosphate (MEP) and the mevalonic acid (MVA) pathways, both of which are involved in terpenoid biosynthesis. Informatic analysis revealed that all of the genes involved in terpenoid biosynthesis showed extensive homology with tracheophyte genes. Phylogenetic analysis indicated that the topology of the phylogenetic trees constructed using genes MEP, DXR and HMGS was in good agreement with the traditional taxonomic classification, but the one constructed using gene FPS was not. Real-time PCR showed that the expression level of these genes was enhanced after the MeJA treatment, as in vascular plants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

01

Opis fizyczny

p.107-118,fig.,ref.rekord w opracowaniu

Twórcy

autor
  • School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China
autor
  • School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China
autor
  • School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China
autor
  • School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China

Bibliografia

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Uwagi

rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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