Nitric oxide affecting root growth, lignification and related enzymes in soybean seedlings

• Autorzy: Zanardo Bomh F.M.L. , Ferrarese M. , Zanardo D. I. L. , Magalhaes J. R. , Ferrarese-Filho O.
• Języki publikacji: EN

Abstrakty

EN

This study analyzed the involvement of nitric oxide (NO) in the root lignification of soybean seedlings. To this end, changes in root cell viability; phenylalanine ammonia-lyase (PAL) and soluble and cell wall bound peroxidase (POD) activities and lignin and hydrogen peroxide (H₂O₂) contents of soybean roots treated with the NO-donor sodium nitroprusside (SNP) and its relationships with root growth were evaluated. Seedlings were cultivated in a nutrient solution supplemented with 5 to 1,000 μM SNP for 24 h. At an extremely low concentration (5 μM), SNP induced root growth and increased lignification and activities of related enzymes (PAL and cell wall-bound POD). At a high concentration (1,000 μM), SNP reduced root growth and lignification (PAL activity and H₂O₂ and lignin contents) and caused a loss of cell viability. Application of potassium ferrocyanide (an analog of SNP that cannot release NO) and PTIO (2-phenyl-4,4,5,5,-tetramethylimidazoleline-1-oxyl-3-oxide, a scavenger of NO) revealed that the inhibitory/stimulatory effects on root lignification may be due to NO itself. These results indicate that NO, depending on its concentration, may act as a stress factor, due to its toxic action, or as a signal molecule, inducing soybean root growth and lignification.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 6
• Opis fizyczny: p.1039-1046,fig.,ref.

Twórcy

autor

Zanardo Bomh F.M.L.

• Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil

autor

Ferrarese M.

• Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil

autor

Zanardo D. I. L.

• Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil

autor

Magalhaes J. R.

• Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil

autor

Ferrarese-Filho O.

• Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil

Bibliografia

• Alexieva V, Sergiev I, Mapelli A, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344
• Arasimowicz A, Floryszak-Wieczorek T (2007) Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Sci 172:876–887
• Baker CJ, Mock NM (1994) An improved method for monitoring cell death in cell suspension and leaf discs assays using Evans blue. Plant Cell Tissue Organ Cult 39:7–12
• Beligni MV, Lamattina L (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210:215–221
• Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Ann Rev Plant Biol 54:519–546
• Bolwell GP, Cramer CL, Lamb CJ, Schuch W, Dixon RA (1986) L-Phenylalanine ammonia-lyase from Phaseolus vulgaris: modulation of the levels of active enzyme by trans-cinnamic acid. Planta 169:97–107
• Clarke A, Desikan R, Hurst RD, Hancock JT, Neill SJ (2000) NO way back: nitric oxide and programmed cell death in Arabidopsis thaliana suspension cultures. Plant J 24:1–13
• Correa-Aragunde N, Graziano M, Chevalier C, Lamattina L (2006) Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato. J Exp Bot 57:581–588
• Delledonne M, Xia Y, Dixon RA, Lamb C (1998) Nitric oxide functions as a signal in plant disease resistance. Nature 394:585–588
• Delledonne M, Polverari A, Murgia I (2003) The functions of nitric oxide-mediated signaling and changes in gene expression during the hypersensitive response. Antioxid Redox Signal 5:33–41
• dos Santos W, Ferrarese MLL, Finger A, Teixeira ACN, Ferrarese-Filho O (2004) Lignification and related enzymes in Glycine max root growth-inhibition by ferulic acid. J Chem Ecol 30:1199–1208
• Enkhardt U, Pommer U (2000) Influence of nitric oxide and nitrite on the activity of cinnamic acid 4-hydroxylase of Zea mays in vitro. J Appl Bot 74:151–154
• Ferrarese MLL, Ferrarese-Filho O, Rodrigues JD (2000a) Ferulic acid uptake by soybean root in nutrient culture. Acta Physiol Plant 22:121–124
• Ferrarese MLL, Rodrigues JD, Ferrarese-Filho O (2000b) Phenylalanine ammonia-lyase activity in soybean roots extract measured by reverse-phase high performance liquid chromatography. Plant Biol 2:152–153
• Ferrarese MLL, Zottis A, Ferrarese-Filho O (2002) Protein-free lignin quantitation in soybean (Glycine max (L.) Merr.) roots. Biologia 57:541–543
• Ferrer MA, Ros Barceló A (1999) Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans. Plant Cell Environ 22:891–897
• Floryszak-Wieczorek J, Milczarek G, Arasimowicz M, Ciszewski A (2006) Do nitric oxide donors mimic endogenous NO-related response in plants? Planta 224:1363–1372
• Gabaldón C, Ros LVG, Pedreño MA, Ros-Barceló A (2005) Nitric oxide production by the differentiating xylem of Zinnia elegans. New Phytol 165:5–7
• Gazaryan IG, Lagrimini LM (1996) Purification and unusual kinetic properties of a tobacco anionic peroxidase. Phytochem 41:1029–1034
• Gouveia CMCP, Souza IF, Magalhães ACN, Martins IS (1997) NO-releasing substances that induce growth elongation in maize root segments. Plant Growth Regul 21:183–187
• Guo P, Cao Y, Li Z, Zhao B (2004) Role of endogenous nitric oxide burst in the resistance of wheat to stripe rust. Plant Cell Environ 27:473–477
• Hu X, Neill SJ, Tang Z, Cai W (2005) Nitric oxide mediates gravitropic bending in soybean roots. Plant Physiol 137:663–670
• Kolodziejek I, Koziol-Lipinska J, Waleza M, Korczynski J, Mostowska A (2007) Aspects of programmed cell death during early senescence of barley leaves: possible role of nitric oxide. Protoplasma 232:97–108
• Neill SJ, Barros R, Bright J, Desikan R, Hancock J, Harrison J, Morris P, Ribeiro D, Wilson I (2008) Nitric oxide, stomatal closure and abiotic stress. J Exp Bot 59:165–176
• Pagnussat GC, Simontacchi M, Puntarulo S, Lamattina L (2002) Nitric oxide is required for root organogenesis. Plant Physiol 129:954–956
• París R, Lamattina L, Casalongué CA (2007) Nitric oxide promotes the wound-healing response of potato leaflets. Plant Physiol Biochem 45:80–86
• Passardi F, Cosio C, Penel C, Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep 24:255–265
• Ros Barceló A, Pomar F, Ferrer MA, Martinez P, Ballesta MC, Pedreño MA (2002) In situ characterization of a NO-sensitive peroxidase in the lignifying xylem of Zinnia elegans. Physiol Plant 114:33–40
• Ros Barceló A, Gabaldón C, Pomar F (2004) Nitric oxide, peroxidase and lignification in higher plants. In: Magalhaes JR, Singh RP, Passos LP (eds) Nitric oxide signaling in higher plants. Studium Press, Houston, pp 277–308
• Sang JR, Jiang MY, Lin F, Xu S, Zhang A, Tan M (2008) Nitric oxide reduces hydrogen peroxide accumulation involved in water stress-induced subcellular anti-oxidant defense in maize plants. J Integr Plant Biol 50:231–243
• Tian X, Lei Y (2006) Nitric oxide treatment alleviates drought stress in wheat seedlings. Biol Plant 50:775–778
• Veitch NC (2004) Horseradish peroxidase: a modern view of a classic enzyme. Phytochem 65:249–259
• Víteček J, Wünschová A, Petřek J, Adam V, Kizek R, Havel L (2007) Cell death induced by sodium nitroprusside and hydrogen peroxide in Tobacco By-2 cell suspension. Biol Plant 51:472–479
• Wang JW, Zheng LP, Wu JI, Tand RX (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15:351–358
• Wojtaszek P (2000) Nitric oxide in plants. To NO or not to NO. Phytochem 54:1–4
• Zottini M, Formentin E, Scattolin M, Carimi F, Schiavo FL, Terzi M (2002) Nitric oxide affects plant mitochondrial functionality in vivo. FEBS Lett 515:75–78