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2011 | 55 | 2 |

Tytuł artykułu

Effect of dexamethasone on proliferation of rat hepatocytes and release of bile acids in vitro

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The influence of dexamethasone (DEX) on proliferative activity and bile salt concentration in primary culture of rat hepatocytes were investigated. Isolated liver cells were cultured with 10⁻³ M of cholesterol, and with 10⁻³ M of cholesterol and addition of 10⁻⁶, 10⁻⁸, or 10⁻¹⁰ M of DEX. After 24, 48, and 72 h, the cell proliferation, bile salt concentration ,and profile were examined. The proliferative activity of control hepatocytes ranged between 0.841 ±0.05 and 0.937 ±0.007. In opposite to 10⁻⁸ M DEX, the addition of 10⁻⁶ and 10⁻¹⁰ M of DEX resulted in a decrease in proliferative activity of cells after 48 h of incubation (0.5 i 9±0.12 and 0.533+0.13, respectively). The presence of DEX resulted in elevation ofbile salt level in samples obtained after 72 h (3.97+1.2 µM/L; 3.42±2.0 µM/L, and 3.52+0.3 µM/L in the presence of 10⁻⁶ M, 10⁻⁸ M, and 10⁻¹⁰ M of DEX, respectively). Proliferative response of rat hepatocytes to DEX depended on dose and incubation time. DEX in the highest concentration intensified the bile salts synthesis much earlier than under other experimental conditions. Among the analysed bile salts, cholic and deoxycholic acids predominated. They were conjugated mostly with taurine and to a lesser extent with glicine.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

55

Numer

2

Opis fizyczny

p.327-331,fig.,ref.

Twórcy

autor
  • Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-033 Lublin, Poland
autor

Bibliografia

  • 1. Bailly-Maitre B., de Sousa G., Boulukos K., Gugenheim J., Rahmani R.: Dexamethasone inhibits spontaneous apoptosis in primary cultures of human and rat hepatocytes via Bcl-2 and Bcl-xL induction. Cell Death Differ 2001, 8, 279-288.
  • 2. Berghe V.W., Francesconi E., De Bossher K., Reche- Rigon M., Hergeman G.: Dissociated glucocorticoids with anti-inflamatory potential repress IL-6 gene expression by a nuclear factor-κB-dependent mechanism. Mol Pharmacol 1999, 56, 797-806.
  • 3. Bobowiec R., Korzecka-Kosior U., Martelli F., Sighieri C.: The role of reduced glutathione in bile formation in sheep. Pol J Vet Sci 1999, 2, 31-36.
  • 4. Bobowiec R., Wójcik M., Martelli F., Ducci M.: Influence of cholestyramine and sunflower oil on the content of plasma bile salts and lipoprotein composition in alloxan induced diabetes in rabbit. Ann Univ Mariae Curie-Sklodowska 2001, 56, 29-37.
  • 5. Chaya D., Fougere-Descharette C., Weiss M.C.: Spontaneous apoptosis in a rat hepatoma cell line and its inhibition by dexamethasone. Cell Death Differ 1996, 3, 97-104.
  • 6. Cressman D.E., Greenbaum L.E., DeAngelis R.A., Aliberto G., Furth E.E., Poli V., Taub R.: Liver failure and defective hapatocyte regeneration in intereukin-6- deficient mice. Science 1996, 274, 1379-1381.
  • 7. Debonera F., Krasinkas A., Gelamn A., Aldeguer X., Que X., Shaked A., Olthoff K.: Dexamethasone inhibits early regenerative response of rat liver after cold preservation and transplantation. Hepatology 2003, 38, 1563-1572.
  • 8. Domingo N., Debono E., Reynier M.O., Crotte C., Thorin B., Charbonnier M., Clerc T., Grilasca J., Chanussot F., Lafont H.: Effect of dexamethasone and linoleic acid on hepatic secretion of biliary lipids and anionic polypeptide factor: in vivo and in vitro studies. Digest 1999, 60, 515- 521.
  • 9. Fausto N., Laird A.D., Webber E.M.: Role of growth factors and cytokines in hepatic regeneration. J Fed Am Soc Exp Biol - FASEB J 1995, 11, 1527-1531.
  • 10. Luttringer O., Theil F.P., Lave T., Wernli-Kuratli K., Guenert T., de Saizieu A.: Influence of isolation procedure, extracellular matrix and dexamethasone on the regulation of membrane transporters gene expression in rat hepatocytes. Biochem Pharmacol 2002, 64, 1637- 1650.
  • 11. Nagy P., Kiss A., Schnur J., Thorgeirsson S.: Dexamethasone inhibits the proliferation of hepatocytes and oval cells but not duct cells in rat liver. Hepatology 1998, 28, 423-429.
  • 12. Princen H.M., Meijer P., Hofstee B.: Dexamethasone regulates bile acids synthesis in monolayer cultures of rat hepatocytes by induction of cholesterol-7 α-hydroxylase. Biochem J 1989, 262, 341-348.
  • 13. Stravitz R.T., Vlahcevic Z.R., Russel T.L., Heizer M.L., Avadhani N.G., Hylemon P.B.: Regulation of sterols-27- hydroxylase and an alternative pathway of bile acid biosynthesis in primary cultures of rat hepatocytes. J Steroid Biochem Mol Biol 1996, 57, 337-347.
  • 14. Suzuki C., Sano N., Takikawa H.: Effect of dexamethasone on biliary excretion of bile AIDS and organic anions in rat. Hepatol Res 2003, 25, 48-54.
  • 15. Tsukamoto I., Kojo S.: Effect of glucocorticoid on liver regeneration after partial hepatectomy in the rat. Gut 1989, 30, 387-390.
  • 16. Turncliff R.Z., Meier P.J., Brouwer K.L.R.: Effect of dexamethasone treatment on the expression and function of transport proteins in sandwich-cultured rat hepatocytes. Drug Metab Dispos 2004, 32, 834-839.
  • 17. Wójcik M., Bobowiec R., Martelli F.: Effect of carotenoids on in vitro proliferation and differentiation of oval cells during neoplastic and non-neoplastic injures in rats. J Physiol Pharmacol 2008, 59, 203-213.
  • 18. Wójcik M., Kosior-Korzecka U., Bobowiec R.: Cytoprotective action of 17ß-oestradiol against iron- induced hepatic oxidative stress in vitro. Bull Vet Inst Pulawy 2010, 54, 259-263.
  • 19. Yamada S., Otto P., Kennedy D., Whayne T.: The effects of dexamethasone on metabolic activity of hepatocytes in primary monolayer culture. In Vitro 1980, 16, 559-570.
  • 20. Yamada Y., Kirillova I., Peschon J.J., Fausto N.: Initiation of liver growth by tumor necrosis factor: deficient liver regeneration in mice lacking type I tumor necrosis factor receptor. Proc Natl Acad Sci USA 1997, 94, 1441-1445.
  • 21. Yamamoto M., Fukuda K., Miura N., Suzuki R., Kodo T., Komatu Y.: Inhibition by dexamthasone of transforming grot factor-ß1-induced apoptosis in rat hepatoma cells; a possible association with Bcl-x1 induction. Hepatology 1998, 28, 959-966.

Typ dokumentu

Bibliografia

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