Screening and molecular characterization of Trichomonas vaginalis genotypes isolated from married women in northern Iran

• Autorzy: Saber N. , Saraei M. , Hajialilo E. , Spotin A. , Mohaghegh M.-A. , Alizadeh S.-A. , Rashidi S. , Javadi A. , Ostadghaderi M. , Heydarian P.
• Języki publikacji: EN

Abstrakty

EN

Trichomonas vaginalis is an anaerobic protozoan parasite that causes trichomonosis in human. It is one of the most common non-viral sexually transmitted infections. It has been found to be most prevalent in patients referred to sexually transmitted disease clinics. In recent years, molecular methods have been used to identify genotypes of this parasite in different parts of the world and so far 6 types of T. vaginalis have identified. The aim of this study was to investigate the prevalence and genotype identification of T. vaginalis from married women in northern Iran. A total of 450 vaginal specimens were taken from married women, referring to health centers in northern Iran. Demographic information of women was collected through a questionnaire. The samples were first examined microscopically and then monitored in Dorsch culture medium for up to 10 days. Actin genes of positive samples were amplified by PCR. Finally, PCR products were used to determine the sequence and genotype of the parasite. Overall, 0.7% (3/450) samples were positive for T. vaginalis. All of the three infected women were housewives. After sequencing, the genotype of these parasites were type H (66.7%) (Accession no; MW414672-MW414673) and type E (33.3%) (Accession no; MW414671). Low prevalence of T. vaginalis in north of Iran indicate high level of hygiene in sexual intercourse and avoiding from high risk sexual behaviors, and also it seems that genotype H is dominant type of the parasite in the study area.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2022
• Tom: 68
• Numer: 3
• Opis fizyczny: p.587-594,fig.,ref.

Twórcy

autor

Saber N.

• Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
• Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

autor

Saraei M.

• Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

autor

Hajialilo E.

• Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
• Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

autor

Spotin A.

• Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
• Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Mohaghegh M.-A.

• Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran

autor

Alizadeh S.-A.

• Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran

autor

Rashidi S.

• Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

autor

Javadi A.

• Department of Social Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

autor

Ostadghaderi M.

• Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

autor

Heydarian P.

• Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
• Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

Identyfikatory

DOI

10.17420/ap6803.465