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2015 | 59 | 2 |

Tytuł artykułu

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to identify the affinity of 10 Staphylococcus strains isolated from table chicken eggs to specific species. Preliminary analysis performed by API ID32 Staph test identified these strains as S. aureus, but they exhibited a negative reaction in the tube coagulase test. Thus, the analysed strains were initially characterised as Staphylococcus aureus-like (SAL). Further characterisation was performed by genoty pie methods, using restriction fragment length polymorphism (RFLP) of the coagulase gene (coa) and sequencing of the gene rpoB. An attempt was also made to identify the isolated Staphylococcus strains by MALDI-TOF mass spectrometry. The results indicated that none of the strains tested belonged to the species S. aureus. The rpoB sequences of five isolates showed the highest sequence similarity to S. haemolyticus, three isolates to S. chromogenes, and one isolate to S. epidermidis. One strain (SAL4) remained unidentified in this analy sis. The results obtained using mass spectrometry were comparable to those based on gene sequence analysis. Strain SAL4, which could not be identified by sequencing, was identified by MALDI-TOF as Staphylococcus chromogenes.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

59

Numer

2

Opis fizyczny

p.235-239,ref.

Twórcy

autor
  • Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
autor
  • Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
  • Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
autor
  • Department of Microbiology, Institute of Biology, University of Bialystok, 15-950 Bialystok, Poland
autor
  • Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

Bibliografia

  • 1. Bergeron M., Dauwalder O., Gouy M., Freydiere A.M., Bes M., Meugnier H., Benito Y., Etienne J., Lina G., Vandenesch F., Boisset S.: Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time- of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 2011, 30, 343-354.
  • 2. Bystroń J., Podkowik M., Piasecki T., Wieliczko A., Molenda J., Bania J.: Genoty pes and enterotoxin gene content of S .aureus isolated from poultry. Vet Microbiol 2010, 144, 498-501.
  • 3. Drancourt M., Raoult D.: rpoB gene sequence-based identification of Staphylococcus species. J Clin Microbiol 2002, 40, 1333-1338.
  • 4. Eigner U., Holfelder M., Oberdorfer K., Betz-Wild U., Bertsch D., Fahr A.M.: Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin Lab 2009, 55, 289-296.
  • 5. Garbacz K., Galiński J.: Atypical Staphylococcus aureus strains defective in coagulase or clumping factor (CF). Post Mikrobiol 2006, 45, 39-43.
  • 6. Heikens E., Fleer A., Paauw A., Florijn A., Fluit A.C.: Comparison of genotypic and phenotypic methods for species- level identification of clinical isolates of coagulase negative staphylococci. J Clin Microbiol 2005, 43, 2286-2290.
  • 7. Luczak-Kadlubowska A., Krzyszton-Russjan J., Hryniewicz W.: Characteristics of Staphylococcus aureus strains isolated in Poland in 1996 to 2004 that were deficient in species-specific proteins. J Clin Microbiol 2006, 44, 4018-4024.
  • 8. Mackay A.D., Quick A., Gillespie S.H., Kibbler C.C.: Coagulase-negative methicillin-resistant Staphylococcus aureus infection. Lancet 1993, 342, 492.
  • 9. Malecka-Mikosz O., Wiczkowski A., Strzelczyk J., Zalewska- Ziob M., Dyla L., Kwasniewski M.: Molecular analysis of methicillin-resistant staphylococci isolated from hospital patients and in the out-patient clinic. Med Dosw Mikrobiol 2004, 56, 1-9.
  • 10. Mellmann K., Becker K., von Eiff C., Keckevoet U., Schumann P., Harmsen D.: Sequencing and staphylococci identification. Emerg Infect Dis 2006, 12, 333-336.
  • 11. Mlynarczyk A., Mlynarczyk G., Szymanowska A., Stanczak J., Luczak M., Jeliaszewicz J.: Application of PCR for detection of coa and nuc genes in methicillin-resistant, coagulase-negative strains of Staphylococcus aureus (MRSA-CN). Med Dosw Mikrobiol 2000, 52, 217-222.
  • 12. Neville L.O., Billington O.J., Kibbler C.C., Gillespie S.H.: Methicillin resistant Staphylococcus aureus without clumping factor, protein A and DNAse. Lancet 1991, 338, 518.
  • 13. Pyzik E., Marek A., Hauschild T.: Characterisation of Staphylococcus aureus and Staphylococcus aureus - like strains isolated from table eggs. Bull Vet Inst Pulawy 2014. 58, 57-63.
  • 14. Szymanowska A., Sawicka-Grzelak A., Mlynarczyk A., Mlynarczyk G.: Evaluation of drug sensitivity and biochemical properties of coagulase -negative S. aureus strains isolated from clinical specimens. Med Dosw Mikrobiol 1993, 45, 11-14.
  • 15. Vandenesch F., Bes M., Lebeau C., Greenland T., Brun Y., Etienne J.: Coagulase-negative Staphylococcus aureus . Lancet 1993, 342, 995-996.
  • 16. Vandenesch F., Lebeau C., Bes M., Brun Y.: Coagulase deficiency in clinical isolates of Staphylococcus aureus involves both transcriptional and post-trancriptional defects. J Med Microbiol 1994, 40, 344-349.
  • 17. Wang Y.R., Chen Q., Cui S.H., Li F.Q.: Characterization of Staphylococcus aureus isolated from clinical specimens by Matrix Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. Biomed Environ Sci 2013, 26, 430-436.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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