The low doses effect of experimental zearalenone (ZEN) intoxication on the presence of Ca2+ in selected ovarian cells from pre-pubertal bitches

• Autorzy: Gajecka M. , Przybylska-Gornowicz B.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to determine the effect of 42-day ZEN intoxication on the presence of Ca2+ in selected ovarian cells from beagle bitches, using the potassium pyroantimonate (PPA) method. Samples were collected from 30 clinically healthy, pre-pubertal, genetically homogeneous animals. The bitches were divided into three groups of 10 animals each: experimental group I - 50 μg ZEN/kg BW (100% NOAEL) administered once daily per os; experimental group II - 75 μg ZEN/kg BW (150% NOAEL) administered once daily per os; control group - placebo containing no ZEN administered per os. An electron microscopic analysis revealed that cells died due to apoptosis, depending on the ZEN dose and the type of cells exposed to intoxication. Lower ZEN doses led to apoptosis-like changes in the cells. Cell death was a consequence of excess Ca2+ accumulation in the mitochondria, followed by cell dysfunction and a decrease in or the absence of mitochondrial metabolic activity in oocytes, follicle cells and interstitial cells in experimental bitches.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 4
• Opis fizyczny: p.711-720,fig.,ref.

Twórcy

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Przybylska-Gornowicz B.

• Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

• Ajduk A (2007) The role of calcium ions in activation of the mammalian embryo development. Postępy Biologii Komórki 34: 715-729.
• Boermans HJ, Leung MC (2007) Mycotoxins and the pet food industry: toxicological evidence and risk assessment. Int J Food Microbiol 119: 95-102.
• Bryden WL (2012) Mycotoxin contamination of the feed supply chain: Implications for animal productivity and feed security. Anim Feed Sci Tech 173: 134-158.
• Denton RM (2009) Regulation of mitochondrial dehydrogenases by calcium ions. Biochim Biophys Acta 1787: 1309-1316.
• Felty Q, Roy D (2005) Estrogen, mitochondria and growth of cancer and non-cancer cells. J Carcinog 4: 1.
• Frizzell C, Ndossi D, Verhaegen S, Dahl E, Eriksen G, Srrlie M, Ropstad E, Muller M, Elliott CT, Connolly L (2011) Endocrine disrupting effects of zearalenone, alpha- and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis. Toxicol Lett 206: 210-217.
• Gajęcka M (2006) The impact of experimental zearalenone mycotoxicosis on the reproductive tract in bitches. Doctoral thesis. University of Warmia and Mazury in Olsztyn, Poland.
• Gajęcka M, Jakimiuk E, Polak M, Otrocka-Domagała I, Janowski T, Zwierzchowski W, Obremski K, Zielonka Ł, Apoznański J, Gajęcki M (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
• Gajęcka M, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2009) The biotransformation of chosen mycotoxins. Pol J Vet Sci 12: 293-303.
• Gajęcka M, Rybarczyk L, Jakimiuk E, Zielonka Ł, Obremski K, Zwierzchowski W, Gajęcki M (2012a) The effect of experimental long-term exposure to low-dose zearalenone on uterine histology in sexually immature gilts. Exp Toxicol Pathol 64: 537-542.
• Gajęcka M, Rybarczyk L, Zwierzchowski W, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2011) The effect of experimental, long-term exposure to low-dose zearalenone mycotoxicosis on the histological condition of ovaries in sexually immature gilts. Theriogenology 75: 1085-1094.
• Gajęcka M, Zielonka Ł, Jakimiuk E, Dąbrowski M, Obremski K, Gorlo G, Mróz M, Gajęcki M (2012b) Diagnostic significance of selected analytical indicators of zearalenone mycotoxicosis in animals. Med Weter 68: 566-570.
• Gellerich FN, Gizatullina Z, Trumbeckaite S, Nguyen HP, Pallas T, Arandarcikaite O, Vielhaber S, Seppet E, Striggow F (2010) The regulation of OXPHOS by extramitochondrial calcium. Biochem Biophys Acta 1797: 1018-1027.
• Gunter TE, Sheu S-S (2009) Characteristics and possible functions of mitochondrial Ca2+ transport mechanisms. Biochem Biophys Acta 1787: 1291-1308.
• Heberer T, Lahrssen-Wiederholt M, Schafft H, Abraham K, Pzyrembel H, Henning KJ, Schauzu M, Braeunig J, Goetz M, Niemann L, Gundert-Remy U, Luch A, Appel B, Banasiak U, Bol GF, Lampen A, Wittkowski R, Hensel A (2007) Zero tolerances in food and animal feed – are there any scientific alternatives? A European point of view on an international controversy. Toxicol Lett 175: 118-135.
• Kiliańska ZM, Miśkiewicz A (2003) Caspases of the vertebrates – their role in apoptosis. Postępy Biologii Komórki 30: 129-152.
• Lewczuk B, Przybylska B, Wyrzykowski Z (1994) Distribution of calcified concretions and calcium ions in the pineal gland. Folia Histochem Cytobiol 32: 243-249.
• Malekinejad H, Colenbrander B, Fink-Gremmels J (2006a) Hydroxysteroid dehydrogenases in bovine and porcine granulosa cells convert zearalenone into its hydroxylated metabolites α-zearalenol and β-zearalenol. Vet Res Commun 30: 445-453.
• Malekinejad H, Van Tol HTA, Colenbrander B, Fink-Gremmels J (2006b) Expression of 3α- and 3β-hydroxy steroid dehydrogenase mRNA in COCs and granulosa cells determines zearalenone biotransformation. Toxicol In Vitro 20: 458-463.
• Maresca M, Fantini J (2010) Some food-associated mycotoxins as potential risk factors in humans predisposed to chronic intestinal inflammatory diseases. Toxicon 56: 282-294.
• Młynarczuk J, Niewiadowska A, Kotwica J (2005) Polychlorinated biphenyls impair FSH-stimulated effect on the secretion of steroids and oxytocin from cultured bovine granulosa cells. Bull Vet Inst Pulawy 49: 411-417.
• Moreira PI, Custódio JBA, Nunes E, Oliveira PJ, Moreno A, Seica R, Oliveira CR, Santos MS (2011) Mitochondria from distinct tissues are differently affected by 17β-estradiol and tamoxifen. J Steroid Biochem Mol Biol 123: 8-16.
• Ozil JP, Markoulaki S, Toth S, Matson S, Banrezes B, Knott JG, Schultz RM, Huneau D, Ducibella T (2005) Egg activation events are regulated by the duration of a sustained [Ca2+]cyt signal in the mouse. Dev Biol 282: 39-54.
• Penning TM (2003) Hydroxysteroid dehydrogenases and pre-receptor regulation of steroid hormone action. Hum Reprod Update 9: 193-205.
• Rizzuto R, Pozzan T (2006) Microdomains of intracellular Ca2+: molecular determinants and functional consequences. Physiol Rev 86: 369-408.
• Słomczyńska M (2004) The effect of phytoestrogens on the reproductive tract. Pol J Vet Sci 7: 223-226.
• Taylor SE, Martin-Hirsch PL, Martin FL (2010) Oestrogen receptor splice variants in the pathogenesis of disease. Cancer Lett 288: 133-148.
• Toth S, Huneau D, Banrezes B, Ozil JP (2006) Egg activation is the result of calcium signal summation in the mouse. Reproduction 131: 27-34.
• Turcotte JC, Hunt PJB, Blaustein JD (2005) Estrogenic effects of zearalenone on the expression of progestin receptors and sexual behavior in female rats. Horm Behav 47: 178-184.
• Ververidis HN, Boscos CM, Stefanakis A, Saratsis P, Stamou AI, Krambovitis E (2004) Serum estradiol-17β, progesterone and respective uterine cytosol receptor concentrations in bitches with spontaneous pyometra. Theriogenology 62: 614-623.
• Wallace KB, Starkov AA (2002) Mitochondrial targets of drug toxicity. Annu Rev Pharmacol Toxicol 40: 353-388.
• Wick SM, Hepler PK (1982) Selective localization of intracellular Ca2+ with potassium antimonite. J Histochem Cytochem 30: 1190-1204.
• Yang SH, Liu R, Perez EJ, Wen Y, Stevens SM Jr, Valencia T, Brun-Zinkernagel AM, Prokai L, Will Y, Dykens J, Koulen P, Simpkins JW (2004) Mitochondrial localization of estrogen receptor beta. Proc Natl Acad Sci USA 101: 4130-4135.
• Zwierzchowski W, Gajęcki M, Obremski K, Zielonka Ł, Baranowski M (2004) The occurrence of zearalenone and its derivatives in standard and therapeutic feeds for companion animals. Pol J Vet Sci 7: 289-293

Uwagi

rekord w opracowaniu