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2013 | 16 | 3 |

Tytuł artykułu

The effect of beta-hydroxy-beta-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

16

Numer

3

Opis fizyczny

p.567-569,ref.

Twórcy

autor
  • Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Cattle Breeding and Milk Quality Evaluation, Faculty of Animal Bioengineering, University of Warmia and Mazury Oczapowskiego 5, 10-718 Olsztyn, Poland
  • Department of Cattle Breeding and Milk Quality Evaluation, Faculty of Animal Bioengineering, University of Warmia and Mazury Oczapowskiego 5, 10-718 Olsztyn, Poland

Bibliografia

  • Chung S, Secombes CJ (1988) Analysis of events occurring within teleost macrophages during the respiratory burst. Comp Biochem Physiol 89 B: 539-544.
  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
  • Nartowska J, Skopińska-Różewska E, Siwicki AK (2004) Nowe immunomodulatory pochodzenia roślinnego. In: Siwicki AK, Skopińska-Różewska E, Świderski F (eds) Immunomodulacja – nowe możliwości w ochronie zdrowia. Wydawnictwo Edycja, Olsztyn, pp 7-21.
  • Peterson AL, Qureshi MA, Ferket PR, Fuller JC Jr (1999) In vitro exposure with beta-hydroxy-beta-methylbutyrate enhances chicken macrophage growth and function. Vet Immunol Immunopathol 67: 67-78.
  • Puchajda-Skowrońska H, Siwicki AK, Wójcik R, Pudyszak K (2006) Effects of 3-hydroxy-3-methylbutyrate (HMB) on selected performance indices and non-specific defense mechanisms in geese. Med Weter 62: 89-92.
  • Rook GA, Steele J, Umar S, Dockrell HM (1985) A simple method for the solubilisation of reduced NBT, and its use as a colorimetric assay for activation of human macrophages by gamma-interferon. J Immunol Methods 82: 161-167.
  • Siwicki AK, Skopińska-Różewska E, Nartowska J, Małaczewska J, Wójcik R, Sommer E, Trapkowska S, Filewska M, Skurzak H (2004) Effect of Immunostim Plus – a standardized fixed combination of Schizandra chinensis with Eleutherococcus senticosus extracts on granulocyte activity and tumour angiogenesis in mice. Bull Vet Inst Pulawy 48: 489-492.
  • Talleyrand V, Dorn A, Frank D, Roth J, Nissen S (1994) Effect of feeding β-hydroxy-β-methylbutyrate on immune function in stressed calves. FASEB J 8: A951.
  • Wagner U, Burkhardt E, Failing K (1999) Evaluation of canine lymphocyte proliferation: comparison of three different colorimetric methods with the 3H-thymidine incorporation assay. Vet Immunol Immunopathol 70: 151-159.
  • Wójcik R (2010) Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. Pol J Vet Sci 13: 181-188.

Typ dokumentu

Bibliografia

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