Effect of nutrient media and phytohormones on in vitro establishment of Bambusa balcooa Roxb.

• Autorzy: Khan H. R. , Burla S. , Siri N. , Lavanya P.
• Języki publikacji: EN

Abstrakty

EN

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company’s green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the quantity and effect of particular plant growth regulating hormones on the Bambusa balcooa.Roxb.

Słowa kluczowe

EN

nutrient; phytohormone; in vitro; establishment; Bambusa balcooa; bamboo; plant growth regulator

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2014
• Tom: 12
• Numer: 1
• Opis fizyczny: p.1-11,fig.,ref.

Twórcy

autor

Khan H. R.

• Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh, India

autor

Burla S.

• Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh, India

autor

Siri N.

• Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh, India

autor

Lavanya P.

• Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh, India

Bibliografia

• [1] Evans D. A, Sharp W. R, Ammirato P. V., Yamada Y. (1983). Hand book of Plant Cell Culture. Macmillan Publishing Company, New York.
• [2] Pierik R. L. M. (1987). Invitro Culture of Higher Plants. Martinus Nijhoff Publishers, Dordrecht.
• [3] Skoog F., Miller C. O., Soc. Exp. Biol. 11 (1957) 118-131.
• [4] Thorpe T. A. (1981). Plant Tissue culture :Methods and Applications in Agriculture. Academic Press Inc., New York.
• [5] Bhojwani S. S., Razdan M. K. (1990). Plant Tissue Culture: Applications and Limitations. Elsevier Science Publishers, Amsterdam.
• [6] Reinert J., Bajaj P. P. S. (eds.) (1977). Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Springer-Verlag, Berlin.
• [7] Street H. S. (ed.) (1973). Plant Tissue and Cell Culture. Blackwell Scientific Publication, Oxford.