Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance

• Autorzy: Ruzauskas M. , Misyte S. , Vaskieviciute L. , Mikniene Z. , Siugzdiniene R. , Klimiene I. , Pikuniene A. , Kucinskiene J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to isolate cultivable gut microbiota from European pond turtles kept at the Lithuanian Zoo and to determine antimicrobial resistance of the isolates. The study subjects included 8 elderly turtles living at the Lithuanian Zoo for about 50 years as well as their offspring – 24 young individuals (1-2 years old) that were hatched at the same zoo. Animals were not exposed by treatment with antimicrobials during the last 3 years. Gut samples were taken from the cloaca and inoculated onto universal media. Isolates then were identified using sequence analysis of 16S rRNA. The antimicrobial susceptibility testing was performed using the agar diffusion method according to Kirby-Bauer. Clinical breakpoints according to CLSI whenever possible, were used for interpretation of susceptibility. Bacterial isolates resistant to at least three antimicrobials of different classes were treated as multi-resistant. Fifty-two bacterial isolates were obtained and identified from turtle gut samples. The most prevalent genera included Aeromonas, Chryseobacterium and Citrobacter. Fifty percent of the isolates obtained from elderly turtles (CI 95% – 19.01-80.99) and 54.8% (CI 95% – 39.75-69.85) of the isolates from young animals were identified as multi-resistant. The most common resistance rates of the isolates from both groups of the turtles were observed toward ampicillin (86.6%), ciprofloxacin (61.5%) and gentamicin (40.4%). The lowest number of resistant isolates were detected toward combination of sulfamethoxazole-trimethoprim (26.9%). The study revealed that European pond turtles kept in captivity are carriers of multi-resistant bacteria however, further studies need to be performed to investigate whether the resistant microorganisms are natural microbiota for this species or they were acquired in the zoo.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 4
• Opis fizyczny: p.723-730,fig.,ref.

Twórcy

autor

Ruzauskas M.

• Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Mickeviciaus g. 9, Kaunas, Lithuania

autor

Misyte S.

• Department of Food Safety and Quality, Veterinary Academy, Tilzes g. 18, Kaunas, Lithuania

autor

Vaskieviciute L.

• Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Mickeviciaus g. 9, Kaunas, Lithuania

autor

Mikniene Z.

• Department of Non-infectious Diseases, Veterinary Academy, Tilzes g. 18, Kaunas, Lithuania

autor

Siugzdiniene R.

• Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Mickeviciaus g. 9, Kaunas, Lithuania

autor

Klimiene I.

• Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Mickeviciaus g. 9, Kaunas, Lithuania

autor

Pikuniene A.

• Department of Cold Blooded Animals, Lithuanian Zoo, Radvilenu 21, Kaunas, Lithuania

autor

Kucinskiene J.

• Department of Food Safety and Quality, Veterinary Academy, Tilzes g. 18, Kaunas, Lithuania

Bibliografia

• Bernardet JF, Vancanneyt M, Matte-Tailliez O, Grisez L, Tailliez P, Bizet C, Nowakowski M, Kerouault B, Swings J (2005) Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals. Syst Appl Microbiol 28: 640-660.
• Calderón G, Garc´ıa E, Rojas P, Garcıa E, Rosso M, Losada A (2011) Chryseobacterium indologenes infection in a newborn: a case report. J Med Case Rep 5: 10.
• Chen FL, Wang GC, Teng SO, Ou TY, Yu FL, Lee WS (2012) Clinical and epidemiological features of Chryseobacterium indologenes infections: analysis of 215 cases. J Microbiol Immunol Infect 46: 425-432.
• Di Ianni F, Dodi PL, Cabassi CS, Pelizzone I, Sala A, Cavirani S, Parmigiani E, Quintavalla F, Taddei S (2015) Conjunctival flora of clinically normal and diseased turtles and tortoises. BMC Vet Res 11: 91.
• Glazebrook JS, Campbell RS (1990) A survey of the diseases of marine turtles in northern Australia. I. Farmed turtles. Dis Aquat Org 9: 83-95.
• Hernandez-Divers SJ, Hensel P, Gladden J, Hernandez-Divers SM, Buhlmann KA, Hagen C, Sanchez S, Latimer KS, Ard M, Camus AC (2009) Investigation of shell disease in map turtles (Graptemys spp.) J Wildl Dis 45: 637-652.
• Igbinosa IH, Igumbor EU, Aghdasi F, Tom M, Okoh AI (2012) Emerging Aeromonas species infections and their significance in public health. Scientific World J 2012: 625023.
• Isonhood JH, Drake M (2002) Aeromonas species in foods. J Food Protect 65: 575-582.
• Kumar S, Mukhopadhyay P, Chatterjee M, Bandyopadhyay MK, Bandyopadhyay M, Ghosh T, Samaddar D (2012) Necrotizing fasciitis caused by Aeromonas caviae. Avicenna J Med 2: 94-96.
• Lescano JG, Quevedo MU, Fernandez VA (2013) Septicemic cutaneous ulcerative disease in a multi-species collection of semi-aquatic turtles. Rev Investig Vet Peru 24: 561-564.
• Loch TP, Faisal M (2015) Emerging flavobacterial infections in fish: A review. J Adv Res 6: 283-300.
• Manchanda V, Sanchaita S, Singh N (2010) Multidrug Resistant Acinetobacter. J Glob Infect Dis 2: 291-304.
• Maravić A, Skočibušić M, Samanić I, Puizina J (2013) Profile and multidrug resistance determinants of Chryseobacterium indologenes from seawater and marine fauna. World J Microbiol Biotechnol 29: 515-522.
• Mauel MJ, Miller DL, Frazier KS, Hines ME 2nd (2002) Bacterial pathogens isolated from cultured bullfrogs (Rana castesbeiana). J Vet Diag Invest 14: 431-433.
• McKew G (2014) Severe sepsis due to Chryseobacterium indologenes in an immunocompetent adventure traveler. J Clin Microbiol 52: 4100-4101.
• Nowakiewicz A, Ziółkowska G, Zięba P, Dziedzic BM, Gnat S, Wójcik M, Dziedzic R, Kostruba A (2015) Aerobic bacterial microbiota isolated from the cloaca of the European
• pond turtle (Emys orbicularis) in Poland. J Wildl Dis 51: 255-259.
• Oros J, Torrent A, Calabuig P, Deniz S (2005) Diseases and causes of mortality among sea turtles stranded in the Canary Islands, Spain (1998-2001). Dis Aquat Organ 63: 13-24.
• Pasquale V, Baloda SB, Dumontet S, Krovacek K (1994) An Outbreak of Aeromonas hydrophila infection in turtles (Pseudemis scripta). Appl Environ Microbiol 60: 1678-1680.
• Pridgeon JW, Klesius PH (2011) Molecular identification and virulence of three Aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in west Alabama (USA) in 2009. Dis Aquat Organ 94: 249-253.
• Rahman M, Colque-Navarro P, Kuhn I, Huys G, Swings J, Möllby R (2002) Identification and characterization of pathogenic Aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in Bangladesh. Appl Environ Microbiol 68: 650-655.
• Ruzauskas M, Siugzdiniene R, Klimiene I, Virgailis M, Mockeliunas R, Vaskeviciute L, Zienius D (2014) Prevalence of methicillin-resistant Staphylococcus haemolyticus in companion animals: a cross-sectional study. Ann Clin Microbiol Antimicrob 13: 56.
• Sarmiento-Ramiırez JM, van der Voort M, Raaijmakers JM, Diéguez-Uribeondo J (2014) Unravelling the microbiome of eggs of the endangered sea turtle Eretmochelys imbricata identifies bacteria with activity against the emerging pathogen Fusarium falciforme. PLoS One. 9: e95206.
• Senior BW, Voros S (1990) Protein profile typing-a new method of typing Morganella morganii strains. J Med Microbiol 33: 259-264.
• Soslau G, Russell JA, Spotila JR, Mathew AJ, Bagsiyao P (2011) Acinetobacter sp. HM746599 isolated from leatherback turtle blood. FEMS Microbiol Lett 322: 166-171.
• Tomas JM (2012) The main Aeromonas pathogenic factors. ISRN Microbiol 2012: 256-261
• Vivas J, Carracedo B, Riaño J, Razquin BE, López-Fierro P, Acosta F, Naharro G, Villena AJ (2004) Behavior of an Aeromonas hydrophila aroA live vaccine in water microcosms. Appl Environ Microbiol 70: 2702-2708.
• von Graevenitz A (2007) The role of Aeromonas in diarrhea: a review. Infection 35: 59-64

Identyfikatory

DOI

10.1515/pjvs-2016-0091