Brain-derived neurotropic factor (RDNF) expression and behavioral response during stimulation of bed nucleus of the stria terminalis (BST) in rats

• Autorzy: Myslinska D. , Majkutewicz I. , Podlacha M. , Ciepielewski Z. , Karnia M. , Wadolowska A. , Rucinski J. , Siudak M. , Zajaczkowski S. , Wrona D.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUNDANDAIMS: The bed nucleus of the stria terminalis (BST) is a part of “the extended amygdala”, a formation responsible for emotional aspects of behavior. The BST is considered as a site of convergence of information from brain regions associated with the control of emotional, cognitive, autonomic and behavioral responsesto stress and noxiousstimuli. On the basis of our previousstudy we assumed that the BST also influenced the primary antitumor immune response. In the present study we investigated the influence of 14-day electrical stimulation of the BST on the brain-derived neurotrophic factor (BDNF) expression and behavioral response. METHODS: Male Wistar rats implanted with electrodes into the BST were divided into groups: BST stimulated and BST sham. The intensity of stimulation current (120–160 µA; 50 Hz) was determined individually for each stimulated rat to induce a behavioral response such a locomotor reaction. The current intensity was raised incrementally in 30 s trials (30 trials/day, 20 s rest between the trials). Behavioral reaction was measured in the Opto Varimex Minor actometer during stimulation procedure. BDNF was detected during immunofluorescence procedure. RESULTS: The stimulation of the BST caused induction of BDNF expression in brain cortical and subcortical motor structures: the frontal primary motor cortex (areas FR1 and FR2), prefrontal cortex, ventral tagmental area, as well as in the central amygdala nucleus and in the hypothalamus: the paraventricular and the supraoptic nuclei, the medial preoptic and the lateral areas positively correlated with the augmentation of the behavioral activity which appeared as locomotor activity (increase in the average number of movements in horizontal and vertical plane). CONCLUSION: This suggests that the behavioral outcome of the BST stimulation, imitating physical exercise, could be responsible for brain BDNF synthesis observed in the study. Supported by: NN303819040.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S77

Twórcy

autor

Myslinska D.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Majkutewicz I.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Podlacha M.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Ciepielewski Z.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Karnia M.

• Department of Biochemistry, Academy of Physical Education and Sport, Gdansk, Poland

autor

Wadolowska A.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Rucinski J.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Siudak M.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Zajaczkowski S.

• Department of Physiology, Medical University of Gdansk, Gdansk, Poland

autor

Wrona D.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland