Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

• Autorzy: Zuber A. , Borowczyk J. , Zimolag E. , Krok M. , Madeja Z. , Pamula E. , Drukala J.
• Języki publikacji: EN

Abstrakty

EN

Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

Słowa kluczowe

EN

poly(L-lactide-co-glycolide); burn; film; skin tissue; skin regeneration; keratinocyte; fibroblast; biomaterial; tissue engineering

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2014
• Tom: 19
• Numer: 2
• Opis fizyczny: p.297-314,fig.,ref.

Twórcy

autor

Zuber A.

• Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland

autor

Borowczyk J.

• Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland

autor

Zimolag E.

• Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland

autor

Krok M.

• Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow 30-059, Poland

autor

Madeja Z.

• Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland

autor

Pamula E.

• Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow 30-059, Poland

autor

Drukala J.

• Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland

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