Physiological and molecular responses of broad bean (Vicia faba L.) to aluminium stress

• Autorzy: Chen Q. , Wu K.-H. , Zhang Y.-N. , Phan X.-H. , Li K.-Z. , Yu Y.-X. , Chen L.-M.
• Języki publikacji: EN

Abstrakty

EN

In this study, the responses of broad bean cultivars resistant (YD) and sensitive (AD) to aluminum (Al) stress were investigated at physiological and molecular levels. The results showed that Al induced more citrate exudation in YD roots than that in AD roots, suggesting that citrate exudation is involved in broad bean Al resistance. The analyses for oxidative stress levels and antioxidant enzyme activities indicated that YD had a strong ability to cope with the oxidative stress induced by Al. To investigate the molecular responses of broad bean to Al stress further, a forward suppression subtractive hybridization cDNA library was constructed to identify Alresponsive genes in YD roots treated with 50-µM Al for a 24-h period. Of the obtained 162 high-quality ESTs, genes related to antioxidant enzymes including copper-zinc superoxide dismutase (SOD), class III peroxidase (POD) and germin-like protein (GEP) were up-regulated. Higher transcription levels of SOD and POD were observed in YD but not in AD roots, which is in agreement with the enhanced activities of antioxidant enzymes in YD roots under Al stress conditions. Furthermore, the up-regulated expression of vha2, encoding a plasma membrane (PM) H⁺-ATPase, and 14-3-3b in YD roots under Al stress were also detected and confirmed by RT-PCR analysis. Western and immunoprecipitation analyses indicated that Al-enhanced expressions and interactions of the PM H⁺-ATPase and 14-3-3 proteins might be involved in the regulation of citrate secretion in YD roots under Al stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2251-2263,fig.,ref.

Twórcy

autor

Chen Q.

• Faculty of Environmental Science and Engineering, Chengong Campus, Kunming University of Science and Technology Chenggong, 650500 Kunming, China
• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

autor

Wu K.-H.

• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

autor

Zhang Y.-N.

• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

autor

Phan X.-H.

• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

autor

Li K.-Z.

• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

autor

Yu Y.-X.

• College of Zoological Science and Technology, Southwest University, 400715 Chongqing, China

autor

Chen L.-M.

• Biotechnology Research Center, Chenggong Campus, Kunming University of Science and Technology, Chenggong, 650500 Kunming, China

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