Apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating and non-haemagglutinating antigenic variants of the RHD (rabbit haemorrhagic disease) virus

• Autorzy: Niedzwiedzka-Rystwej P. , Tokarz-Deptula B. , Deptula W.
• Języki publikacji: EN

Abstrakty

EN

This paper attempts to study the dynamics of apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating (Vt97, Triptis, Hartmannsdorf) and non-haemagglutinating (Pv97, 9905 RHDVa) antigenic variants of the RHD virus. The pathogenicity of those antigenic variants was also assessed by recording the mortality of the infected animals. The animals were infected with antigenic variants and blood was sampled at hour 0,4,8,12,24,36 p.i. and the percentage of apoptotic granulocytes and lymphocytes was measured with the use of flow cytometry. The results of the study showed that apoptosis is included during RHDV infection, as the number of apoptotic granulocytes and lymphocytes increases throughout the experiment; depending on the antigenic variant, apoptosis joins in at 4-8-12 h p.i. and lasts until 24-36 h p.i. Furthermore, the mortality of rabbits infected with the examined strains of RHD virus varied from 30% to 100%. This study performed for the first time in this manner, indicates the importance of apoptosis during infection with the RHD virus.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 2
• Opis fizyczny: p.223-229,fig.,ref.

Twórcy

autor

Niedzwiedzka-Rystwej P.

autor

Tokarz-Deptula B.

autor

Deptula W.

Bibliografia

• Alonso C, Oviedo JM, Martin-Alonso JM, Diaz E, Boga JA, Parra F (1998) Programmed cell death in the pathogenesis of rabbit hemorrhagic disease. Arch Virol 143: 321-332.
• Berninger ML, House C (1995) Serologic comparison of four isolates of rabbit hemorrhagic disease virus. Vet Microbiol 47: 157-165.
• Capucci L, Chasey D, Lavazza A, Westcott D (1996) Preliminary characterization of a non-haemagglutinating strain of rabbit haemorrhagic disease virus from the United Kingdom. J Vet Med B 43: 245-250.
• Capucci L, Fallacara F, Grazioli S, Lavazza A, Pacciarini ML, Brocchi E (1998) A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant. Virus Res 58: 115-126.
• Chasey D, Lucas MH, Westcott DG, Sharp G, Kitching A, Hughes SK (1995) Development of a diagnostic approach to the identification of rabbit haemorrhagic disease. Vet Rec 137: 158-160.
• Farnós O, Rodriguez D, Valdés O, Chiong M, Parra F, Toledo JR, Fernández E, Lleonart R, Suárez M (2007) Molecular and antigenic characterization of rabbit hemorrhagic disease virus isolated in Cuba indicates a distinct antigenic subtype. Arch Virol 152: 1215-1221.
• Fitzner A (2006) Charakterystyka molekularna wirusa RHD z uwzględnieniem szczepów o odmiennym fenotypie. Habilitation thesis. PIW Puławy, Poland.
• Fitzner A, Kęsy A, Niedbalski W, Paprocka G, Walkowiak B (1996) Identification of polypeptide VP560 of national isolates of viral haemorrhagic disease of rabbits. Med Weter 52: 303-305.
• Geisbert TW, Hensley LE, Gibb TR, Steele KE, Jaax NK, Jahrling PB (2000) Apoptosis induced in vitro and in vivo during infection by Ebola and Marburg viruses. Lab Invest 80: 171-186.
• Hukowska-Szematowicz B (2006) Charakterystyka immunologiczno- genetyczna wybranych szczepów wirusa RHD (rabbit haemorrhagic disease). Doctoral thesis, WNP US, Szczecin, Poland.
• Jung JY, Lee BJ, Tai JH, Park JH, Lee YS (2000) Apoptosis in rabbit haemorrhagic disease. J Comp Pathol 123: 135-140.
• Kęsy A, Fitzner A, Niedbalski W, Paprocka W, Walkowiak B (1996) A new variant of the viral haemorrhagic disease of rabbits virus. Rev Sci Tech 15: 1029-1035.
• Le Gall-Reculé G, Zwingelstein F, Laurent S, de Boisseson C, Portejoie Y, Rasschaert D (2003) Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterisation of RHDV antigenic variants. Arch Virol 148: 65-81.
• Matiz K, Ursu K, Kecskeméti S, Bajmócy E, Kiss I (2006) Phylogenetic analysis of rabbit haemorrhagic disease virus (RHDV) strains isolated between 1988 and 2003 in eastern Hungary. Arch Virol 151: 1659-1666.
• McIntosh MT, Behan SC, Mohamed FM, Lu Z, Moran KE, Burrage TG, Neilan JG, Ward GB, Botti G, Capucci L, Metwally SA (2007) A pandemic strain of calicivirus threatens rabbit industries in the Americas. Virol J 4: 96.
• Niedźwiedzka-Rystwej P, Deptuła W (2010) Non-specific immunity in rabbits infected with 10 strains of the rabbit haemorrhagic disease virus with different biological properties. Cent Eur J Biol 5: 613-632.
• Nowaczyk P (2007) Zjawiska immunologiczne, apoptoza i badania wirusologiczne wykonane biologią molekularną u królików immunizowanych EBHS (European Brown Hare Syndrome) virus. Doctoral thesis, WNP US, Szczecin, Poland.
• Oem JK, Lee KN, Roh IS, Lee KK, Kim SH, Kim HR, Park CK, Joo YS (2009) Identification and characterization of rabbit hemorrhagic disease virus genetic variants isolated in Korea. J Vet Med Sci 71: 1519-1523.
• Parrino J, Hotchkiss RS, Bray M (2007) Prevention of immune cell apoptosis as potential therapeutic strategy for severe infections. Emerg Infect Dis 13: 191-198.
• Prieto JM, Martin JM, Espi A, Parra F (2000) A new non-haemagglutinating strain of rabbit haemorrhagic disease virus. In: Brocchi E, Lavazza A (eds) Proceedings of the 5th International Congress of the European Society of Veterinary Virology “Veterinary Virology in the New Millenium”, Brescia, Italy, pp. 204-205.
• San-Miguel B, Alvarez M, Culebras JM, Gonzalez-Gallego J, Tunon MJ (2006) N-acetyl-cysteine protects liver from apoptotic death in an animal model of fulminant hepatic failure. Apoptosis 11: 1945-1957.
• Schirrmeier H, Reimann I, Kollner B, Granzow H (1999) Pathogenic, antigenic and molecular properties of rabbit haemorrhagic disease virus (RHDV) isolated from vaccinated rabbits: detection and characterization of antigenic variants. Arch Virol 144: 419-735.
• Tian L, Liao J, Li JW, Zhou WR, Zhang XL, Wang HN (2007) Isolation and identification of a non-haemagglutinating strain of rabbit hemorrhagic disease virus from China and sequence analysis for the VP60 gene. Virus Genes 35: 745-752.
• van de Bildt MW, van Bolhuis GH, van Zijderveld F, van Riel D, Drees JM, Osterhaus AD, Kuiken T (2006) Confirmation and phylogenetic analysis of rabbit hemorrhagic disease virus in free-living rabbits from the Netherlands. J Wildl Dis 42: 808-812.
• Tokarz-Deptuła B (2009) Immunity phenomena in rabbits infected with the RHD (rabbit haemorrhagic disease) virus. Pol J Environ Stud 7: 1-81.
• Tokarz-Deptuła B, Deptuła W (2004) The immunity during immunization with the viral haemorrhagic disease (VHD) in rabbits. Centr Eur J Immunol 29: 58-62.
• Tokarz-Deptuła B, Deptuła W, Kęsy A (2002) Plague rabbit and the associated immune phenomena. Med Weter 58: 497-500.
• Tokarz-Deptuła B (1998) Kształtowanie się wybranych wskaźników odporności nieswoistej u królików po zakażeniu wirusem VHD (viral haemorrhagic disease). Doctoral thesis, PIW Puławy.
• Tokarz-Deptuła B, Hukowska B, Deptuła W (2003) Dynamic alternations in selected indices of non-specific cell-mediated immunity in rabbits experimentally infected with VHD (viral haemorrhagic disease) virus. Pol J Vet Sci 6: 70-73.