Molecular methods for identification of monophasic Salmonella Typhimurium strains

• Autorzy: Madajczak G. , Dera-Tomaszewska B. , Wasiak M. , Chrost A. , Szych J.
• Języki publikacji: EN

Abstrakty

EN

Two molecular biology methods were used to differentiate Salmonella enterica 1,4,[5],12:i:- strains: “Salmonella Check&Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT result “Salmonella 1,4,[5],12:i:-” were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium, the same identification was obtained in mPCR. Reference Salmonella strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as Salmonella genovar, in mPCR – as Salmonella O:4, H:i other than Salmonella Typhimurium, the same like Salmonella Farsta, recognized incorrectly in CT as Salmonella Typhimurium.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2015
• Tom: 64
• Numer: 4
• Opis fizyczny: p.383-386,ref.

Twórcy

autor

Madajczak G.

• Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland

autor

Dera-Tomaszewska B.

• Department of Medical Microbiology, Medical University of Gdansk, Gdansk, Poland

autor

Wasiak M.

• Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland

autor

Chrost A.

• Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland

autor

Szych J.

• Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland

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Identyfikatory

DOI

10.5604/17331331.1185238