The emergence of different functionally equivalent PAH degrading microbial communities from a single soil in liquid PAH enrichment cultures and soil microcosms receiving PAHs with and without bioaugmentation

• Autorzy: Piubeli F.A. , Dos Santos L.G. , Fernandez E.N. , Da Silva F.H. , Durrant L.R. , Grossman M.J.
• Języki publikacji: EN

Abstrakty

EN

Polycyclic aromatic hydrocarbon (PAHs) are common soil contaminants of concern due to their toxicity toward plants, animals and microorganisms. The use of indigenous or added microbes (bioaugmentation) is commonly used for bioremediation of PAHs. In this work, the biodegradation rates and changes in the bacterial community structure were evaluated. The enrichment culture was useful for unambiguously identifying members of the soil bacterial community associated with PAH degradation and yielded a low diversity community. No significant difference in the rate of PAH degradation was observed between the microcosm receiving only PAHs or PAHs and bioaugmentation. Moreover, identical matches to the bioaugmentation inoculum were only observed at the initial stages of PAH degradation on day 8. After 22 days of incubation, the substantial degradation of all PAHs had occurred in both microcosms and the PAH contaminated soil had statistically significant increases in Alphaproteobacteria. There were also increases in Betaproteobacteria. In contrast, the PAH contaminated and bioaugmented soil was not enriched in PAH degrading Proteobacteria genera and, instead, an increase from 1.6% to 8% of the population occurred in the phylum Bacteroidetes class Flavobacteria, with Flavobacterium being the only identified genus. In addition, the newly discovered genus Ohtaekwangia increased from 0% to 3.2% of the total clones. These results indicate that the same soil microbial community can give rise to different PAH degrading consortia that are equally effective in PAH degradation efficiency. Moreover, these results suggest that the lack of efficacy of bioaugmentation in soils can be attributed to a lack of persistence of the introduced microbes, yet nonetheless may alter the microbial community that arises in response to PAH contamination in unexpected ways.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 3
• Opis fizyczny: p.365-375,fig.,ref.

Twórcy

autor

Piubeli F.A.

• Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain

autor

Dos Santos L.G.

• Department of Food Science - FEA, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

autor

Fernandez E.N.

• Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain

autor

Da Silva F.H.

• Laboratory of Molecular Biology, DGE-Federal University of Sao Carlos (DGE/UFSCar), Sao Carlos, SP, Brazil

autor

Durrant L.R.

• Department of Food Science - FEA, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

autor

Grossman M.J.

• Department of Food Science - FEA, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

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Identyfikatory

DOI

10.21307/pjm-2018-046