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Czasopismo

2013 | 25 | 2 |

Tytuł artykułu

Identification of new Trichoderma strains with antagonistic activity against Botrytis cinerea

Warianty tytułu

PL
Identyfikacja nowych szczepów Trichoderma o aktywności antagonistycznej przeciwko Botrytis cinerea

Języki publikacji

EN

Abstrakty

EN
The antagonistic activity of 52 isolates of Trichoderma spp. against Botrytis cinerea was tested in in vitro conditions using the dual culture technique. The results revealed that all of the Trichoderma isolates had the ability to inhibit the mycelial growth of grey mould. The percentage reduction in the growth of Botrytis cinerea after six days of incubation at 25°C varied between 45-78%. The isolates Tr43 and Tr52 showed the highest antagonistic activity (Tr43 - 76%; Tr52 - 78%). Biochemical and molecular identification indicated that both isolates were T. atroviride. The isolates showed differences in the utilisation of 11 to 96 different carbon sources. Additional biochemical tests revealed the ability of Tr43 and Tr52 to produce siderophores, indole-3-acetic acid and chitinases. Neither of the isolates gave positive results regarding phosphate solubilisation on Pikovskaya’s medium.
PL
52 izolaty grzybów z rodzaju Trichoderma zostały przebadane z użyciem techniki podwójnych kultur w celu oceny ich antagonistycznego oddziaływania przeciwko Botrytis cinerea. Wszystkie spośród badanych izolatów hamowały wzrost szarej pleśni. Wartość inhibicji wzrostu B. cinerea po 6 dniach inkubacji w temperaturze 25°C wynosiła 45-78%. Największą aktywność antagonistyczną wykazały izolaty Tr43 i Tr52 (Tr43 - 76%, Tr52 - 78%). Izolaty te zostały zidentyfikowane jako Trichoderma atroviride. Na podstawie identyfikacji biochemicznej izolatów Tr43 i Tr52 z użyciem systemu do identyfikacji mikroorganizmów BIOLOG stwierdzono różnice w utylizacji 11, spośród 96 źródeł węgla. Dodatkowe testy biochemiczne wykazały zdolność izolatów Tr43 i Tr52 do syntezy sideroforów, kwasu indoilo- 3-octowego i chitynaz. Nie stwierdzono zdolności do rozpuszczania związków fosforu na podłożu wg Pikowskiej.

Słowa kluczowe

Wydawca

-

Czasopismo

Rocznik

Tom

25

Numer

2

Opis fizyczny

p.123-132,fig.,ref.

Twórcy

autor
  • Department of Pomology, Research Institute of Horticulture, Pomologiczna 18, 96-100 Skierniewice, Poland
autor
  • Department of Pomology, Research Institute of Horticulture, Pomologiczna 18, 96-100 Skierniewice, Poland
autor
  • Department of Pomology, Research Institute of Horticulture, Pomologiczna 18, 96-100 Skierniewice, Poland
autor
  • Department of Pomology, Research Institute of Horticulture, Pomologiczna 18, 96-100 Skierniewice, Poland
autor
  • Department of Pomology, Research Institute of Horticulture, Pomologiczna 18, 96-100 Skierniewice, Poland

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-041d67db-ed84-4dc7-901d-3422531ed9c7
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