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2018 | 64 | 4 |

Tytuł artykułu

Detection of Cryptosporidium parvum DNA in fecal samples of infected cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in the Philippines using loop-mediated isothermal amplification method

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Fecal DNA samples from 17 cattle and 38 water buffaloes found to be infected with Cryptosporidium oocysts using Kinyoun acid fast stain from a previous study, were subjected to loop-mediated isothermal amplification (LAMP) assay using specific primers for Cryptosporidium parvum (C. parvum) from three municipalities, Maria, Baler and San Luis of the province of Aurora in the Philippines. Results of the fecalysis using Kinyoun acid fast stain and LAMP assay were compared with the PCR results of the examined farmer/owner who raised these animals to determine the possible zoonoses of C. parvum between the farmers and their animals. Using LAMP assay, only 41% (7/17) were positive in cattle and 76% (29/38) in water buffaloes. Out of the seven LAMP positive cases in cattle, 86% (6/7) came from Maria and 14% (1/7) from Baler. Out of 29 LAMP positive cases in water buffaloes, 62% (18/29) came from Maria, 24% (7/29) from Baler and 14% (4/29) from San Luis. Comparing with the earlier results for probable zoonoses of C. parvum between the farmers and their animal was determined. Eight farmers that were positive in PCR and with their water buffaloes, positive in LAMP assay were detected to have C. parvum. Only one farmer with his cattle was detected positive of Cryptosporidium spp. in PCR, however, it was negative in LAMP assay hence, a non-parvum species might infected the farmer and the animal.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

64

Numer

4

Opis fizyczny

p331-338,fig.,ref.

Twórcy

  • College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
autor
  • College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
autor
  • College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines
  • Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines
  • Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

Bibliografia

  • [1] Thomson S., Hamilton C.A., Hope J.C., Katzer F., Mabbott N.A., Morrison L.J., Innes E.A. 2017. Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Veterinary Research 48: 42. https://doi.org/10.1186/s13567-017-0447-0
  • [2] Harp J.A., Woodmansee D.B., Moon H.W. 1990. Resistance of calves to Cryptosporidium parvum: effects of age and previous exposure. Infection and Immunity 58: 2237-2240.
  • [3] Harp J.A., Goff J.P. 1998. Strategies for the control of Cryptosporidium parvum infection in calves. Journal of Dairy Science 81: 289-294.
  • [4] Rinaldi L., Musella V., Condoleo R., Saralli G., Veneziano V., Bruni G., Condoleo R.U., Cringoli G. 2007. Giardia and Cryptosporidium in water buffaloes (Bubalus bubalis). Parasitology Research 100: 1113-1118. https://doi.org/10.1007/s00436-006-0397-3
  • [5] Amer S., Zidan S., Feng Y., Adamu H., Li N., Xiao L. 2013. Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt. Veterinary Parasitology 191: 123-127. https://doi.org/10.1016/j. vetpar.2012.08.015
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  • [7] Ghazy A.A., Abdel-Shafy S., Shaapan R.M. 2015. Cryptosporidiosis in animals and man: 1. Taxonomic classification, life cycle, epidemiology and zoonotic importance. Asian Journal of Epidemiology 8: 48-63. http://dx.doi.org/10.3923/aje.2015.48.63
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  • [16] Endo S., Komori T., Ricci G., Sano A., Kamei K., Franco M., Miyagi M., Nishimura K. 2004. Detection of gp43 of Paracoccidiodes brasiliensis by the loopmediated isothermalamplification (LAMP) method. FEMS Microbiology Letters 234: 93-97.
  • [17] Karanis P., Thekisoe O., Kioptsi K., Ongerth J., Igashi I., Inoe N. 2007. Development and preliminary evaluation of LAMP procedure for sensitive detection of Cryptosporidium oocyts in fecal and water samples. Journal of Applied and Environmental Microbiology 73:5660-5662. doi:10.1128/AEM.01152-07
  • [18] Nagamine K., Watanabe K., Ohtsuka K., Hase T., Notomi T. 2001. Loop-mediated isothermal amplification reaction using a non denatured template. Clinical Chemistry 47:1742-1743.
  • [19] Mori Y., Nagamine K., Tomita N., Notori T. 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochemical and Biophysical Research Communications 289: 150-154.
  • [20] Villanueva M.A., Domingo C.Y.J., Abes N.S., Mingala C.N. 2009. Incidence and risk factors of Cryptosporidium spp. infection in water water buffaloes confined in a communal management system in the Philippines. Internet Journal of Veterinary Medicine 8(1).
  • [21] Hart M.L., Meyer A., Johnson P.J., Ericsson A.C. 2015. Comparative evaluation of DNA extraction methods from feces of multiple host species for downstream next-generation sequencing. PLoS One 10: e0143334. https://doi.org/10.1371/journal.pone.0143334
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  • [23] Fayer R., Morgan U., Upton S. 2000. Epidemiology of Cryptosporidium: Transmission, detection and identification. International Journal for Parasitology 26: 1305-1322.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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