EN
Dendritic cells were generated from the monocytes, which were separated from the blood with the use of monoclonal antibodies and immunomagnetic separation. The monocytes were cultivated in cell culture in the presence of IL-4 and GM-CSF. Their morphology changed towards dendritic cells after 24 h, as was observed in the light and scanning electron microscopes. The generation of dendritic cells from monocytes may provide adequate numbers of cells in clinical studies and prophylaxis.