Analysis of cytoplasmic variation in a cucumber germplasm collection using chloroplast microsatellite markers

• Autorzy: Hu J.-B. , Li J.-W. , Zhou X.-Y.
• Języki publikacji: EN

Abstrakty

EN

Nine PCR-based markers were developed from the microsatellites in non-coding regions of chloroplast genome of Cucumis sativus and used to detect chloroplast DNA variation. These markers successfully detected intraspecific polymorphism among 37 cucumber accessions containing Chinese native germplasms (CNGs) and non-Chinese germplasms (NCGs). Each marker detected between two and four alleles and the diversity value of the makers ranged from 0.105 to 0.528. Based on the data from allele size variation, a total of 17 distinct haplotypes were identified from the 35 accessions (excluding the two accessions possessing null genes). Three haplotypes were prevalent among CNGs but most NCGs had unique haplotype. No identical haplotype was found between CNGs and NCGs, reflecting lack of exchange of CNGs with others in the 60–80s of last century. A wild species (C. hystrix Chakr.) tested herein shared a haplotype with some CNGs, suggesting that it could be the ancestry of C. sativus or at least had a common ancestral lineage. The genetic relationship among the 37 cucumber accessions was further analyzed through construction of dendrogram based on Jaccard coefficient of similarity obtained from the allele sizes. All the CNGs were clustered into a group (containing the wild accession) that distinctly differed from the other four groups containing NCGs. This result agreed with the findings above obtained from haplotype analysis. Our research documented here will offer useful information for cucumber breeding.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 5
• Opis fizyczny: p.1085-1089,fig.,ref.

Twórcy

autor

Hu J.-B.

• College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Li J.-W.

• College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Zhou X.-Y.

• College of Horticulture, Northeast Agricultural University, Harbin 150030, China

Bibliografia

• Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580. doi:10.1093/nar/27. 2.573
• Bryan GJ, McNicoll J, Ramsay G, Meyer RC, DeJong WS (1999) Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. Theor Appl Genet 99:859–867. doi:10.1007/s001220051306
• Chung SM, Staub JE, Chen JF (2006) Molecular phylogeny of Cucumis species as revealed by consensus chloroplast SSR marker length and sequence variation. Genome 49:219–229. doi: 10.1139/G05-101
• Chung SM, Gordon VS, Staub JE (2007) Sequencing cucumber (Cucumis sativus L.) chloroplast genomes identifies differences between chilling-tolerant and -susceptible cucumber lines. Genome 50:215–225. doi:10.1139/G07-003
• Dane F (1983) Cucurbits. In: Tanksley SD, Orten TJ (eds) Isozymes in plant genetics and breeding, Part B. Elsevier, Amsterdam, pp 369–390
• Dijkhuizen A, Kennard WC, Havey MJ, Staub JE (1996) RFLP variation and genetic relationships in cultivated cucumber. Euphytica 90:79–87
• Horejsi T, Staub JE (1999) Genetic variation in cucumber (Cucumis sativus L.) as assessed by random amplified polymorphic DNA. Genet Resour Crop Evol 46:337–350. doi:10.1023/A:10086505 09966
• Ishii T, Mori N, Ogihara Y (2001) Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species. Theor Appl Genet 103:896–904. doi:10.1007/ s001220100715
• Lalitha S (2000) Primer premier 5. Biotechnol Softw Internet Rep 1:270–272. doi:10.1089/152791600459894
• Li XX, Zhu DW, Du YC, Shen D, Kong QS, Song JP (2004) Studies on genetic diversity and phylogenetic relationship of cucumber (Cucumis sativus L.) germplasm by AFLP analysis. Acta Hortic Sin 3:309–314 (in Chinese)
• Mu SQ, Gu XF, Zhang SP, Wang XW, Wang Y (2008) Genetic diversity of cucumber (Cucumis sativus L.) germplasm by SSR. Acta Hortic Sin 35:1323–1330 (in Chinese)
• Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325. doi: 10.1093/nar/8.19.4321
• Nei M (1986) Molecular evolutionary genetics. Columbia University Press, New York
• Provan J, Corbett G, Waugh R, McNicol JW, Morgante M, Powell W (1996) DNA fingerprints of rice (Oryza sativa) obtained from hypervariable chloroplast simple sequence repeats. Proc Biol Sci 263:1275–1281. doi:10.1098/rspb.1996.0187
• Provan J, Powell W, Hollingsworth PM (2001) Chloroplast microsatellites: new tools for studies in plant ecology and evolution. Trends Ecol Evol 16:142–147. doi:10.1016/S0169-5347(00) 02097-8
• Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1 user guide. Exeter Software, Setauket
• Tatlioglu T (1993) Cucumber Cucumis sativus L. In: Kalloo G, Bergh BO (eds) Genetic improvement of vegetable crops. Pergamon Press Ltd, Tarrytown, pp 197–234
• Wang J, Xu Q, Miao MM, Liang GH, Zhang ZM, Cheng XH (2007) Analysis of genetic relations of cucumber (Cucumis sativus L.) germplasm by ISSR markers. Mol Plant Breed 5:677–682 (in Chinese)

Uwagi

Rekord w opracowaniu