The effect of mycorrhization on the growth, flowering, content of chloroplast pigments, saccharides and protein in the leaves of Sinningia speciosa (Lodd.) Hiern

• Autorzy: Janowska B. , Rybus-Zajac M. , Horojdko M. , Andrzejak R. , Siejak D.

Warianty tytułu

PL

Wpływ mikoryzacji na wzrost, kwitnienie Sinningia speciosa (Lodd.) Hiern oraz zawartość barwników chloroplastowych, cukrowców i białka w liściach

• Języki publikacji: EN

Abstrakty

EN

The research was conducted on two cultivars of Sinningia speciosa (Lodd.) Hiern: ‘Defiance’ and ‘Blanche de Meru’. Plants were cultivated with or without symbiosis with endomycorrhizal fungi. In order to evaluate the biochemical changes in the leaves of Sinningia speciosa at the vegetative growth stage the content of chlorophyll a+b, carotenoids, protein and saccharides was determined. Plant growth parameters, such as height, diameter, number of leaves and number of initiated flower buds, were determined when first flower was developed. Mycorrhizal plants of Sinningia ’Defiance’ and ‘Blanche de Meru’ had more flower buds, 66.7 and 57.1%, respectively. The mycorrhization had a positive influence on the content of chlorophyll a+b in the leaves of Sinningia speciosa ‘Defiance’, whereas in the Blanche de Meru cultivar this dependence was observed only in the ninth and tenth week of cultivation. At the vegetative stage the mycorrhized plants had a higher content of carotenoids in their leaves, except for the tenth week of cultivation in the Defiance and the seventh and tenth weeks of cultivation in the Blanche de Meru cultivar. The mycorrhization did not influence the content of protein in the cultivars under investigation, except for the ninth week of cultivation. The highest content of saccharides in the leaves of Defiance and Blanche de Meru cultivars was noted at the beginning of vegetation and it was similar in the mycorrhized and non-mycorrhized plants.

PL

W badaniach oceniano wpływ grzybów endomikoryzowych na zmiany, w fazie wzrostu wegetatywnego, zawartości chlorofilu a+b, karotenoidów, białka i cukrowców w liściach Sinningia speciosa (Lodd.) Hiern ‘Defiance’ i ‘Blanche de Meru’. Jakość roślin (wysokość, średnica, liczba liści i pąków kwiatowych) określono w fazie, gdy na roślinach rozwinął się pierwszy kwiat. Mikoryzacja siningii odmian Defiance i Blanche de Meru stymulowała tworzenie się pąków kwiatowych. Na roślinach mikoryzowanych rozwinęło się średnio o 66,7 i 57,1% więcej pąków kwiatowych, odpowiednio dla Defiance i Blanche de Meru., Mikoryzacja miała pozytywny wpływ na zawartość chlorofilu a+b w liściach odmiany Defiance. U odmiany Blache de Meru zależność tą obserwowano tylko w 9 i 10 tygodniu uprawy. W fazie wzrostu wegetatywnego mikoryzowane rośliny miały większą zawartość karotenoidów w liściach, za wyjątkiem odmiany Defiance w 7. tygodniu uprawy i odmiany Blanche de Meru w 7. i 10. tygodniu uprawy. Mikoryzacja nie miała wpływu na zawartość białka w liściach badanych odmian, za wyjątkiem 9 tygodnia uprawy. Podwyższoną zawartość cukrowców u obu odmian odnotowano pod koniec fazy wegetatywnej, zarówno u mikoryzowanych jak niemikoryzowanych roślin.

Słowa kluczowe

EN

mycorrhization; mycorrhizal fungi; plant growth; flowering; chloroplast pigment; saccharide; protein; leaf; biochemical change; Sinningia speciosa; ornamental plant

• Wydawca: -
• Czasopismo: Acta Agrophysica
• Rocznik: 2016
• Tom: 23
• Numer: 2
• Opis fizyczny: p.213-223,fig.,ref.

Twórcy

autor

Janowska B.

• Department of Ornamental Plants, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland

autor

Rybus-Zajac M.

• Department of Plant Physiology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Horojdko M.

• Department of Plant Physiology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Andrzejak R.

• Department of Phytopathology and Seed Science Technology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland

autor

Siejak D.

• Department of Ornamental Plants, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland

Bibliografia

• Allen M.F., 1991. The ecology of mycorrhizae. Cambridge University Press, Cambridge, England.
• Arnon D.J., 1949. Cooper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physol., 24, 1-15.
• Auge R.M., 2001. Water relation, drought and vesicular-arboscular mycorrhizal symbiosis. Mycorrhiza, 11, 3-42.
• Bethenfalvay G.J., Brown M., Franson R., 1988. Glycine-Glomus-Bradyrhizobium symbiosis. Plant Physiol., 94, 723-728.
• Bethlenfalvay G.J., Ames R.N., 1987. Comparison of two methods for quantifying extraradical mycelium of vesicular arbuscular mycorrhizal fungi. Soil Sci. Soc. Am. J., 51, 834-837.
• Björnesjö K., 1955. Analysis of protein-bound serum polysaccharides with anthrone reagent. Scand. J. Clin. Lab. Inves., 6, 147-152.
• Bolandnazar S., Aliasgarzad N., Neishabury M.R., Chaparzadeh N., 2007. Mycorrhizal colonization improves onion (Allium cepa L.) yield and water use efficiency under water deficit condition. Sci. Hort., 114, 11-15.
• Borkowska B., 2002. Growth and photosynthetic activity of micropropagated strawberry plants inoculed with endomycorrhizal fungi (AMF) and growing under drought stress. Acta Physiol. Plant., 24(4), 365-370.
• Bowen G.D., Rovira A.D., 1998. The rhizosphere and its management to improve plant growth. Adv. Agron., 66, 1-102.
• Bradford M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem., 72, 248-254.
• Costa M..D., Pereira O.L., Kasuya M.C.M., Borges A.C., 2002. Ectomicorrizas: a face oculta das florestas. Biotecno Ci Desenvol., 29, 38-46.
• Dixon R.K., Rao M.V., Garg V., 1994. Water relations and gas exchange of mycorrhizal Leucaena leucocephala seedlings. J. Trop Forest. Sci., 6, 542-552.
• Dood J.C., 2000. The role of arboscular mycorrhizal fungi in agro- and natural ecosystems. Outlook an Agric., 29, 55-62.
• El-Tahomy W., Schnitzler W.H., El-Behairy U., El-Beltagy M.S., 1999. Effect of VA mycorrhiza on improving drought and chilling tolerance of bean plants (Phaseolus vulgaris L.). J. Appl. Bot-Angew. Bot., 73, 178-183.
• Estrada-Luna A.A., Davies F.T., Egilla J.N., 2000. Mycorrhizal fungi enhancement of growth and gas exchange of micropropagated guava plantlets (Psidium guajava L.) during ex vivo acclimatization and plants establishment. Mycorrhiza, 10, 1-8.
• Falkowski G., Koniarski M., Matysiak B., 2009. Zastosowanie ekokompostu i szczepionek endomikoryzowych w uprawie pięciornika krzewiastego (Potentilla fruticosa L.) ‘Gold Drop’ (Effect of ecocompost and endomycorrhizal inoculaon growth of Potentilla fruticosa L. ‘Gold Drop’). Zesz. Probl. Post. Nauk Roln., 539, 159-168 (in Polish).
• Gaur A., Adholeya A., 2005. Diverse response of five ornamental plant species to mixed indigenous and single isolate arbuscular-mycorrhizal inocula in marginal soil amended with organic matter. J. Plant Nutr., 28, 707-723.
• Guillemin J.P., Gianinazzi S., Trouvelot A.,1992. Screening of arbuscular endomycorrhizal fungi for establishment of micropropagated pineapple plants. Agronomie, 12, 831-836.
• Hiscox J.D., Israelstam G.F., 1997. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot., 57, 1332-1334.
• Hooker J.H., Gianinazzi S., Vestberg M., Barea J., Atkinson D., 1994. The application of arbuscular fungi to micropropagation systems: an opportunity to reduce chemical inputs. Agric. Sci. in Finland, 3, 227-232.
• Janowska B, 2013. Effect of growth regulators on flower and leaf yield of the calla lily (Zantedeschia Spreng.). Hort. Sci. (Prague), 40(2), 78-82.
• Janowska B., Andrzejak R., Kosiada T., Trelka T., Frąszczak B., 2013. Effect of mycorrhization on the flowering of the Zantedeschia albomaculata (Hook.) Baill. cv. Albomaculata. Hort. Sci. (Prague), 40(3), 126-130.
• Janowska B., Stanecki M., 2013. Effect of rhizome soaking in a mixture of BA and GA3 on the earliness of flowering and quality of the yield of flowers and leaves in the calla lily (Zantedeschia Spreng.). Acta Sci. Pol., Hort. Cult., 12(2), 3-12.
• Kennedy A.C., 1998. The rhizosphere and spermosphere. In: Sylvia D.M., Fuhrmann J.J., Hartel P.G. Zuberer D.A. (Ed.). Principles and applications of soil microbiology. Prentice Hall, Upper Saddle River, New Jersey, USA, 389-407.
• Klämbt D., 1976. Cytokinin effects on protein synthesis of in vitro systems of higher plants. Plant Cell Physiol., 17(1), 73-76.
• Lovato P.E., Gianinazzi-Pearson V., Trouvelot A, Gianinzzi S., 1996. The state of art of mycorrhizas and micropropagation. Adv. Hort. Sci., 10, 46-52.
• Lynch J.M.,1990. Beneficial interactions between micro-organisms and roots. Biotechnol. Adv., 8(2), 335-346.
• Mathur N, Vyas A., 1995. Influence of VAM on net photosynthesis and transpiration of Ziziphus mauritiana. Plant Physiol., 147, 328-330.
• Matysiak B., 2009. Zastosowanie szczepionek endomikoryzowych w czasie rozmnażania Ilex x meserveae ‘Blue Boy’ przez sadzonki oraz ich wpływ na dalszy wzrost i rozwój roślin (Effect of endomycorrhizal inocula during propagation on the growth following transplanting of Ilex x meserveae ‘Blue Boy’ cutting). Zesz. Probl. Post. Nauk Roln., 539, 499-506 (in Polish).
• Morte A., Lovisolo C, Schubert A., 2000. Effect of drought stress on growth and water relations of the mycorrhizal association Helianthemum almeriense – Terfezia claveryi. Mycorrhiza, 10(3), 115-119.
• Nowak J., 2009. Effects of mycorrhization and phosphorus nutrition on nutrient uptake, growth and flowering of China aster (Callistephus chinensis L. Nees) cultivated on ebb-and-flow benczes. Acta Agrobot., 62(1), 77-81.
• Panwar J.P.S., 1991. Effect of VAM and Azospirillum brasilense on photosynthesis, nitrogen metabolism and grain yield in what. Ind. J. Plant Physiol., 34(4), 357-361.
• Phillips J.M., Hayman D.S., 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc., 55, 158-161.
• Porcel R., Barea J.M., Ruiz-Lozano J.M., 2003. Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol., 157, 135-143.
• Tagekami T., Yoshiba K., 1997. Specific interaction of cytokinin binding protein with 40S ribosomal subunits in the presences of cytokinin in vitro. Plant Cell Physiol., 18(2), 337-346.
• Wang B., Qiu Y.L., 2006. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza., 16, 299-363.