AGRO - Yadda

1

Regeneration of plantlets and tetraploidy induction in Pseudostellaria heterophylla

100%

Ye Z., Wang Y. Y., Tian H. Q.

Acta Biologica Cracoviensia. Series Botanica

|

2009

|

tom 51

|

nr 2

13-18

This study was aimed at developing an efficient protocol for regeneration of Pseudostellaria heterophylla plantlets and induction of polyploidy. Calli of P. heterophylla (Miq) from stems, leaves and buds as explants could not differentiate into plantlets. However, young embryo segments used as primary explants produced embryonic calli on MS medium containing 5.0 mg/L 2,4-D and 0.5 mg/L KT. After the embryonic calli with granular protuberances were transferred to MS medium containing 0.5 mg/L BA, they developed shoots and then rooted to form plantlets. Polyploidy was induced when embryonic calli were placed in liquid MS medium containing 0.5% colchicine for 4 days, followed by culturing in solid medium to induce differentiation. Polyploidy was identified by the number of chromosomes and the size of plantlet stomata. The tetraploid plantlets produced larger root tubers than the diploid plantlets.

2

Physiological characterizations of three barley genotypes in response to low potassium stress

88%

Ye Z., Zeng J., Li X., Zeng F., Zhang G.

Acta Physiologiae Plantarum

|

2017

|

tom 39

|

nr 10

3

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

88%

Ye Z., Peng H., Fang Y., Feng J., Yang D. S.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 3

In our previous study, we demonstrated that azurin could selectively trigger apoptosis in human osteosarcoma cell line U2OS cells. However, the rate of apoptosis (35.8 ± 3.2%) is not very high, and azurin is too expensive to obtain readily. To solve these problems, we constructed a eukaryotic expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid pcDNA3.1(+)/azurin into U2OS cells. RT-PCR and Western blot analysis validated the successful transfection and the expression of the azurin-HA protein. Conspicuous apoptosis of the transfected cells was detected by flow cytometry (FCM) and the DNA ladder test. The apoptosis rate reached 64.3 ± 13.1%. The transcriptional levels of the Bax and p53 genes increased significantly in U2OS cells transfected with pcDNA3.1(+)/azurin, but the Bcl-2 mRNA level decreased. There was no difference in the levels of Bcl-xl mRNA and Survivin mRNA. We propose that the transfection of the recombinant plasmid pcDNA3.1(+)/azurin can significantly induce apoptosis in U2OS cells. This is closely associated with the up-regulation of the transcriptional level of the Bax and p53 genes, and the down-regulation of that of the Bcl-2 gene.

4

Infectivity and screening of anti-piperaquine genes in mice infected with piperaquine-sensitive and piperaquine-resistant Plasmodium berghei

76%

Yi G., Zhou L., Ye Z., Huang X., Lu F., Guo H.

Acta Parasitologica

|

2019

|

tom 64

|

nr 3

5

Accumulation and localization of cadmium in moso bamboo (Phyllostachys pubescens) grown hydroponically

64%

Li S., Islam F., Peng D., Chen J., Wang Y., Wu Y., Ye Z., Yan W., Lu K., Liu D.

Acta Physiologiae Plantarum

|

2015

|

tom 37

|

nr 03

Hydroponics experiment was conducted to identify cadmium (Cd) tolerance and phytoextraction potential of moso bamboo (Phyllostachys pubescens) seedlings grown under different levels of Cd. Application of Cd adversely affected the overall growth and ultrastructural characteristics of moso bamboo. At the highest Cd concentration (400 lM), the growth of moso bamboo seedlings was significantly inhibited, and Cd concentrations in leaves, stems and roots reached the maximum of 25.6, 129.8 and 377 mg kg-1, respectively. Scanning electron microscopy (SEM) revealed that the excessive Cd concentrations caused formation of abundant inclusions in the root and stem. The ultrastructural analysis using transmission electron microscopy (TEM) showed that the excessive Cd concentrations caused abnormal-shaped chloroplasts, disappearance of endoplasmic reticulum, and shrinkage of nucleus and loss of thylakoid membranes. It is suggested that use of moso bamboo as experimental material provides a new perspective for remediation of heavy metal-contaminated soil due to its deep root system and larger biomass. However, mechanisms of Cd uptake and accumulation as well as metal interactions within the plant cell need to be investigated further.

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Regeneration of plantlets and tetraploidy induction in Pseudostellaria heterophylla

Physiological characterizations of three barley genotypes in response to low potassium stress

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

Infectivity and screening of anti-piperaquine genes in mice infected with piperaquine-sensitive and piperaquine-resistant Plasmodium berghei

Accumulation and localization of cadmium in moso bamboo (Phyllostachys pubescens) grown hydroponically