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1

Detection of porcine endogenous retrovirus in xenotransplantation

100%
Gola J., Mazurek U.
Reproductive Biology
|
2014
|
tom 14
|
nr 1
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The possibilities of practical application of transgenic mammalian species generated by somatic cell cloning in pharmacology, veterinary medicine and xenotransplantology

75%
Samiec M., Skrzyszowska M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Cloning of genetically-modified mammals to produce: 1) novel animal bioreactors expressing human genes in rens, urinary bladder and the male accessory sex glands, as well as 2) porcine organs suitable in pig-to-human xenotransplantology, could offer new advantages for biomedical purposes. So too does the generation and/or multiplication of genetically-engineered cloned animals in order to produce: 3) physiologically-relevant animal models of serious monogenic human diseases and 4) prion disease-resistant small as well as large animals (i.e., rodents, ruminants). The basic purpose of this paper is to overview current knowledge deciphering the possibilities of using transgenic specimens created by somatic cell nuclear transfer in medical pharmacology, veterinary medicine, agriculture, transplantational medicine and immunology.
3

Production of transgenic pigs expressing human alpha 1,2-fucosyltransferase to avoid humoral xenograft rejection

75%
Lipinski D., Jura J., Zeyland J., Juzwa W., Maly E., Kalak R., Bochenek M., Plawski A., Szalata M., Smorag Z., Slomski R.
Medycyna Weterynaryjna
|
2010
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tom 66
|
nr 05
316-322
The use of animals as a source of organs and tissues for xenotransplantation may overcome the growing shortage of human organ donors. However, the presence of xenoreactive antibodies in humans, directed against the swine Gal antigen present on the surface of xenograft donor cells, leads to the complement activation and an immediate xenograft rejection as a consequence of hyperacute reaction. In order to prevent a hyperacute rejection, it is possible to alter the swine genome with human genes modifying the set of the donor’s cell surface proteins. The aim of this study was to prepare a pCMVFut genetic construct and then introduce it into the swine genome in order to obtain transgenic pigs expressing human α1,2-fucosyltransferase and thereby avoid a humoral xenograft rejection. The pCMVFut gene construct containing the human gene encoding α1,2-fucosyltransferase enzyme under the human cytomegalovirus immediate early promoter was introduced by microinjection into a male pronucleus of a fertilized porcine oocyte. The screening procedure involved isolating genomic DNA from microsections of pigs’ ears, the amplification of two PCR fragments and the entire sequencing of positive samples. The mapping of the transgene was performed by fluorescence in situ hybridisation (FISH) and transgene expression, while its impact on the reduction of the Gal epitope level on the surface of pig cells was assessed by flow cytometry of primary cultured skin fibroblasts. The influence of the human complement was measured by testing the sensitivity of nontransgenic and transgenic cells to complement-mediated cytotoxicity upon exposure to human serum. As a result of this experiment, the founder male pig was obtained with the transgene mapping to chromosome 14q28. An RT-PCR analysis revealed the expression of the HT gene in different tissues of transgenic pigs. A flow cytometry analysis revealed a reduction in the level of epitop Gal on the cell surface of skin fibroblasts isolated from transgenic pigs. The complement-mediated cytotoxicity assay showed increased viability of transgenic cells in comparison with nontransgenic ones, which confirmed the protective influence of HT expression. In this study we demonstrated that the constitutive transgenic expression of human H-transferase (α1,2-fucosyltransferase) can decrease the amount of Galα1,3Gal (Gal epitope) on the surface of pig cells, which is consistent with the results of other researchers. The expression of α1,2-fucosyltransferase modified the cell surface carbohydrate phenotype of transgenic pig cells, resulting in the expression of the universally tolerated 0 blood group antigen (H antigen) and a subsequent reduction in the expression of Gal epitope, as evaluated by flow cytometry analysis. Apart from the principal data, the flow cytometry analysis revealed no significant differences between the Gal epitope level achieved by CMVFUT heterozygous boar founder TG 1154 and transgene homozygous pig 433 from the F2 generation. The flow cytometry results were confirmed by the cytotoxicity assay. We found no statistical difference in the survival rate between transgenic homozygous and heterozygous cells under the influence of 50% human serum with an active complement system. Both homozygous and heterozygous cells had the same level of lysis protection.
4

Pig somatic cloning - methods, pregnancy maintenance and uses

75%
King T. J., De Sousa P. A.
Animal Science Papers and Reports. Supplement
|
2004
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tom 22
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nr 1
5

Development of pig cloning studies: past, present and future

63%
Samiec M.
Journal of Animal and Feed Sciences
|
2004
|
tom 13
|
nr 2
211-238
6

Immune modulation in xenotransplantation

63%
Boksa M., Zeyland J., Slomski R., Lipinski D.
Archivum Immunologiae et Therapiae Experimentalis
|
2015
|
tom 63
|
nr 3
7

Double transgenic pigs with combined expression of human alpha1,2-fucosyltransferase and alpha-galactosidase designed to avoid hyperacute xenograft rejection

63%
Zeyland J., Wozniak A., Gawronska B., Juzwa W., Jura J., Nowak A., Slomski R., Smorag Z., Szalata M., Mazurek U., Lipinski D.
Archivum Immunologiae et Therapiae Experimentalis
|
2014
|
tom 62
|
nr 5
8

Transplant tolerance: current insights and strategies for long-term survival of xenografts

63%
Liu L., He C., Liu J., Lv Z., Wang G., Gao H., Dai Y., Cooper D. K. C., Cai Z., Mou L.
Archivum Immunologiae et Therapiae Experimentalis
|
2018
|
tom 66
|
nr 5
9
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Obtainment of transgenic porcine fibroblast cell lines for the purpose of xenotransplantation

63%
Hryhorowicz M., Nowak A., Grzeskowiak B., Zeyland J., Slomski R., Lipinski D.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
10

The effect of recombinant human alpha-1,2-fucosyltransferase and alpha-galactosidase A on the reduction of alpha-gal expression in the liver of transgenic pigs

63%
Wiater J., Karasinski J., Slomski R., Smorag Z., Wartalski K., Gajda B., Jura J., Romek M.
Folia Biologica
|
2020
|
tom 68
|
nr 4
11

Human CD46, CD55 and CD59 expression influence on swine transgenic fetal fibroblasts' survivability in the presence of human complement components

51%
Zeyland J., Lipinski D., Slomski R.
Annals of Animal Science
|
2012
|
tom 12
|
nr 4
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Unconventional uses of pigs

51%
Klocek C., Nowicki J., Magiera A., Penar W.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2019
|
tom 58[4]
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