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Derzsy's disease occurring in geese is caused by a virus from the Parvoviridae family called the Derzsy's disease virus or goose parvovirus. For thirty years Derzsy's disease has been observed in Poland and currently it is the cause of large economic losses. The main objective of the study was come up with the molecular characteristics of Derzsy's disease virus strains isolated from field cases. The samples were taken from 65 goose flocks. The geese, 1.5-6-weeks-of-age, were suspected of being affected with Derzsy's disease. Seven viral isolates were isolated from visceral organs of the geese and on the basis of cytopathic changes in goose embryo fibroblasts culture and pathological changes in goose embryos were classified as Derzsy's disease virus strains. One band of 806 bp size, which is characteristic of Derzsy's disease virus, was demonstrated in PCR. On the basis of phylogenetic analysis, the strains were classified as goose parvovirus. The strains were qualified to two phylogenetic groups: vaccine and low pathogenicity strains and field strains. The calculated similarity between the studied strains ranged from 92.3% to 100% and between European strains from 91.3% to 99.5%. On the basis of the authors' research it can be claimed that Polish Derzsy's disease strains differ slightly from each other but all of the strains have a common European origin.
Vaccine B-38 strain of Derzsy’s disease virus (DDV) was used in the study. The virus was lyophilized after the multiplication in the culture of GEF. Sucrose — glutamine, gelatine — sucrose, peptone — lactose and SPGA solutions were applied as stabilizators. The lyophilizates were kept at 4°C for 24 months. The concentrations of the virus in the samples were estimated periodically. It was found that peptone — lactose stabilizator was the most effective protector of DDV from inactivation. The virus was dissolved in Eagle’s fluid, PBS and commercial solvent. The dissolved vaccine was kept at 4°C, 21°C and —20°C for 24 h. The titre of the vaccine was determined immediately after the dissolving and then after 2, 4, 6 and 24 h. Storage of the vaccine at 21° or 4°C for more than 2 h resulted in a significant drop of its titre. Eagle’s fluid used as a disolvent did not differ significantly from the commercial solvent.
The aetiological agent of Derzsy’s disease is a parvovirus (GPV) belonging to the Parvoviridae family, genera Dependovirus. Currently, the mortality rate of Derzsy’s disease is much lower than it was before the introduction of vaccination. The most common form is the chronic one. Therefore, cases of Derzsy’s disease in geese in 1998-2011 were analyzed. The study was carried out in 122 bird flocks. The number of birds in the flocks ranged from 1,200 to 12,000, and most flocks consisted of 3,000-5,000 geese. Most goslings in the flocks were 2-5 weeks old. The presence of antibodies against GPV was detected in all flocks of birds up to 5 weeks of age. The first symptoms, such as reluctance to eat and drink, difficulty in locomotion, paralysis and diarrhoea, usually appeared in the first two weeks of life. The mortality of geese in each year ranged from 13.95% to 42.45%. Among the geese examined, the most frequent pathological changes were observed in the liver, in the form of oedema (22%) and hyperaemia (41%). In 33% of geese a significant degree of emaciation was noted. Derzsy’s disease was confirmed by the PCR detection of the virus in the visceral organs in 36 of the flocks examined. Derzsy’s disease was reported most frequently in the following voivodeships: Greater Poland, Podlaskie, Lublin, Lower Silesian, and Lubusz. In these areas, despite annual prophylactic vaccination, Derzsy’s disease was observed in geese aged 1- 6 weeks, usually between 2.5-3.5 weeks of age. Additionally, using the multiplex PCR, a mixed infection with GPV and goose circovirus was found in 6 flocks. Research aimed at developing a recombinant vaccine against Derzsy’s disease and a new enzymatic ELISA was started last year. To sum up, the analysis of the results shows that Derzsy’s disease remains a health threat in young goose flocks.
Derzsy’s disease is a common disease of waterfowl poultry. The etiological agent of the disease is the goose parvovirus (GPV), also called Derzsy’s disease virus (DDV), that belongs to Parvoviridae family. The genome of the virus is represented by single stranded DNA about 5106 nt long with a molecular density of 1.38 g/ml. The disease occurs among domestic and wild geese as well as Mullard and Barbarie ducks. The GPV infection is spread horizontally through feces excreted by infected birds. There are no reliable data about transmission through the respiratory and conjunctiva route. The disease’s progress and symptoms depend on the age of birds and their immunological status. The incubation period of the disease is approximately 5 days in the case of fully susceptible 1-7-day-old goslings and the course of the disease is acute. Among older goslings the incubation period is prolonged to about 10 days and the disease course is chronic. Presently, Derzy’s disease is diagnosed among goslings older then 3-weeks-of-age with low levels of maternal antibodies. The most obvious symptoms are retarded growth and incomplete feathering. During post mortem examination the anatomopathological changes characteristic for Derzsy’s disease are found in the liver and cardiac muscle. The laboratory diagnostics of Derzsy’s disease is mainly focused on clinical and anatomopathological examinations as well as on serologic, virologic, and histopathologic examinations, together with the application of molecular biology methods. The only method of Derzsy’s disease control is vaccination with living vaccine based on goose parvovirus strain and with bivalent inactivated vaccine containing goose parvovirus strain and Muscovy duck parvovirus strain. Laying geese are vaccinated before and during the middle of the laying season, and the immunity persists during whole laying season.
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