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Wystepowanie wiroida latentnego chmielu w Polsce

100%

Cajza M.

Progress in Plant Protection

2000

tom 40

nr 2

730-732

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The use of reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

100%

Cajza M., Wypijewski K., Pospieszny H.

Journal of Plant Protection Research

1997

tom 37

nr 1-2

52-58

The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.

Wirusy, wiroidy i fitoplazmy na brzoskwiniach

59%

Zawadzka B.

Sad Nowoczesny

2005

tom 33

nr 09

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Progress in Plant Protection

1 Sad Nowoczesny

2 Cajza M.

1 Pospieszny H.

1 Wypijewski K.

1 Zawadzka B.

1 2005

1 2000

1 1997

Wyniki wyszukiwania

Wystepowanie wiroida latentnego chmielu w Polsce

The use of reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

Wirusy, wiroidy i fitoplazmy na brzoskwiniach