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Bacterial wilt caused by Clavibacter michiganensis subsp. insidiosus (Cmi) is an integral part of chronic vascular wilt of alfalfa. The aim of the presented study was to determine the virulence of Cmi strains and later on to evaluate the level of resistance of alfalfa varieties registered in the Czech Republic and of some standard check varieties. Three methods of inoculation were compared at greenhouse conditions. The severity of the disease was determined on the basis of symptoms appearing on the cross section of the roots. The Cmi strain 12/5/98 and its re-isolates showed a high level of virulence. Freeze-dried isolates lost their virulence. The best differentiation of tested varieties according to the level of resistance was obtained by using successive inoculation on cotyledons, stems and roots. Of tested alfalfa varieties, Vernal and Roamer were classified as resistant and Nitranka, Orca and Europe as the most susceptible. Alfalfa varieties (Czech and European) registered in the Czech Republic recently proved lower disease severity in comparison with ones registered formerly (r = –0.538, p (0.05) = 0.553). The above mentioned fact evidences the progress of Cmi resistant alfalfa breeding in Europe
The effect of crude extracts of neem (Azadirachta indica) leaf, neem seed and garlic (Allium sativum) at concentrations ranging from 5% to 30% of the material in 100 ml of Potato Dextrose Agar on mycelial growth of Fusarium oxysporum f. sp. lycopersici was assessed. All the extracts inhibited mycellial growth at various levels. Dry neem seed extract gavel 100% inhibition of mycelial growth. Fresh neem leaf extract reduced mycelial growth with increasing concentration while in garlic there were no differences in growth inhibition among the various concentrations used. However garlic extracts decreased sporulation with increasing concentration and cultures grown on extract amended agar plates remained viable.
Fusarium species are causal agents of fungal diseases occurring frequently in numerous agriculturally important plants, including potato, garlic and are one of the common pathogens of tomato, causing root rot in the west part of Iran. Therefore, the objectives of this study were to isolate and identify disease-causing Fusarium species from infected tomatoes based on the morphological and molecular characteristics. Twenty-five isolates of Fusarium were obtained from infected root of tomato plants collected from the fields in different regions of western Iran. Based on morphological features, the strains were classified into four following Fusarium species: F. oxysporum, F. redolens, F. proliferatum and F. verticillioides. The phylogenetic trees based on tef1 and tub2 dataset clearly distinguished closely related species. All of the isolates were evaluated for their pathogenicity on healthy tomato seedlings in the greenhouse. This is the first report on molecular identification of Fusarium species isolated from tomato plants cultivated in Iran.
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