Wyniki wyszukiwania

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Morphometric analysis of the small intestine in wild type mice C57BL-6J - a developmental study

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Gulbinowicz M., Berdel B., Wojcik S., Dziewiatkowski J., Oikarinen S., Mutanen M., Kosma V. M., Mykkanen H., Morys J.

Folia Morphologica

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2004

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tom 63

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nr 4

Recently the increasing prevalence of gastrointestinal diseases, including neoplasm, has resulted in the necessity of characterising not only the tumours, but also healthy mucosa. Research into the morphological changes of healthy mucosa under different experimental conditions, including drugs, special diets and the use of probiotic bacteria, is greatly facilitated by the availability of animal models. In spite of the widespread use of mice in gastrointestinal research, there is a lack of information on the qualitative and quantitative histological characteristics of the intestinal mucosa of the mouse. The aim of this study was to assess the morphological characteristics and the postnatal development of the small intestine of wild type mice — C57BL/6J. The mice were aged either 5 weeks or 12 weeks. The 12-week-old mice had been weaned at the age of 5 weeks. After dissection the small intestine was divided into 5 equal portions and randomly chosen microscopical sections from each were stained with haematoxylin and eosin. The parameters describing the morphology of the small intestine (villus height, depth of the crypt, villus width near the crypt, width of the villus connective tissue near the crypt, thickness of the muscular layer and the height of the enterocytes and their nuclei) were evaluated under a light microscope. In both age groups the height and width of the villi decreased, while the thickness of the muscular layer increased in the distal direction. The height of the enterocytes decreased and the height of the enterocyte nucleus increased towards the colon in both age groups. The depth of the crypts was greater in the younger animals than in the older ones. Our data provides the baseline morphological description of the small intestinal mucosa in wild type mice, strain C57BL/6J, which can be used as a reference for testing the influence of drugs, toxins, nutrients and inborn mutations on the mouse intestine.

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Analysis of rabbit fed on wild type and genetic modified potato tubers

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Zuk M., Kulma A., Wilczynski G., Prescha A., Szopa J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

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Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Differential dynamic proteome analysis of the oxidative stress response in Arabidopsis wild type and catalase mutants

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Jaques S., Gevaert K., Van Breusegem F.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

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In vitro culture promotes partial autonomous endosperm development in unfertilized ovules of wild-type Arabidopsis thaliana var. Columbia

72%

Rojek J., Kuta E., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

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Associations of maize protein bodies with cytoskeleton, membranes, and ribosomes in the endosperm of wild type and opaque-2 mutant

72%

Stankovic B., Abe S., Azama K., Shibata K., Ito Y., Weidner S., Davies E.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 4

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Wild type root cultures of Scutellaria barbata

58%

Wilczanska-Barska A., Krauze-Baranowska M., Majdan M., Glod D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 4

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Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana [L.] Heynh plants

58%

Todorova D., Genkov T., Vaseva-Gemisheva I., Alexieva V., Karanov E., Smith A., Hall M.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

The levels of three endogenous cytokinin equivalents: zeatin (Z), iso-pentenyladenine (iP) and dihydrozeatin (dZ) in two Arabidopsis thaliana (L.) Heynh genotypes - wild type (wt) and ethylene-insensitive mutant (eti5), were compared using enzyme immunoassay (ELISA). Cytokinin content was measured after exposure to low (4 °C for 24 h in darkness) or high temperature (38 °C for 24 h in darkness). Measurements were performed immediately and 24, 48 and 120 h after treatments. It was found that at normal growth conditions eti5 plants contained more endogenous cytokinins compared to the wild type. At both temperature treatments mutant plants had decreased total cytokinin levels. Wild-type plants treated with high temperature (HT) exhibited reduced total cytokinins (with the exception of rates at 48 h), while low temperature (LT) treatment resulted in elevated total amount of the studied equivalents (except at 24 h). The obtained results suggested that HT had greater effect on cytokinin levels than LT since it caused more profound changes in the total content. We assume that this was due to the natural chilling tolerance of Arabidopsis plants.

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Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin [Lupinus angustifolius L.]

58%

Boersma J. G., Pallotta M., Li C., Buirchell B. J., Sivasithamparam K., Yang H.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 2

Ograniczanie wyników

Morphometric analysis of the small intestine in wild type mice C57BL-6J - a developmental study

Analysis of rabbit fed on wild type and genetic modified potato tubers

Differential dynamic proteome analysis of the oxidative stress response in Arabidopsis wild type and catalase mutants

In vitro culture promotes partial autonomous endosperm development in unfertilized ovules of wild-type Arabidopsis thaliana var. Columbia

Associations of maize protein bodies with cytoskeleton, membranes, and ribosomes in the endosperm of wild type and opaque-2 mutant

Wild type root cultures of Scutellaria barbata

Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana [L.] Heynh plants

Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin [Lupinus angustifolius L.]