During Isolation of total rilmnucleic acids from white lupin (Lupinus albus) and their subsequent separation by 10% polyacrylamide gel electrophoresis, a fast migrating RNA band is very well separated. The nucleotide sequence analysis of 76 nucleotide long sequence with many modified nucleosides was found to be identical with that of tyrosine specific tRNA of yellow lupin seeds (Lupinus luteus) and wheat germ (Trtiicum aestivum). Also this tRNATyr is identical with plant amber suppressor tRNA. 'I'he presented approach offers a very rapid method of purification of plant tRNA with UAG suppressor activity.
A suspension culture of white lupin cells has been established, and proteins of the exocellular matrix analysed. Based on homologies of N-terminal amino-acid sequences, three stress- or defence-related proteins: acidic class III chitinase, polygalacturonase-inhibiting protein, and germinóxalate oxidase, secreted by lupin cell culture, were identified.